5-Hydroxyindoleacetic acid in cerebrospinal fluid reflects serotonergic damage induced by 3,4-methylenedioxymethamphetamine in CNS of non-human primates

George Ricaurte, L. E. DeLanney, S. G. Wiener, I. Irwin, J. W. Langston

Research output: Contribution to journalArticle

Abstract

This study examined whether 5-hydroxyindoleacetic acid (5-HIAA) in cerebrospinal fluid (CSF) could be used to detect serotonergic damage induced by (±)-3,3-methylenedioxymethamphetamine (MDMA) in the central nervous system (CNS) of non-human primates. Monkeys were administered toxic doses of MDMA; two weeks later, the animals were lightly anesthetized with ether and CSF was obtained by means of cervical puncture. Later that same day, the animals were killed for direct determination of CNS serotonin and 5-HIAA concentrations. Monkeys with 73-94% depletions of serotonin and 5-HIAA in brain and 42-45% depletions of serotonin and 5-HIAA in the spinal cord had a 60 ± 7% reduction of 5-HIAA in CSF, without any change in homovanillic acid (HVA) or 3-methoxy-4-hydroxyphenethyleneglycol (MHPG). These findings indicate that CSF 5-HIAA can be employed to detect central serotonergic damage produced by MDMA in non-human primates, and suggest that CSF 5-HIAA may be useful for detecting MDMA-induced neuronal damage in humans.

Original languageEnglish (US)
Pages (from-to)359-363
Number of pages5
JournalBrain Research
Volume474
Issue number2
DOIs
StatePublished - Dec 6 1988

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N-Methyl-3,4-methylenedioxyamphetamine
Hydroxyindoleacetic Acid
Primates
Cerebrospinal Fluid
Central Nervous System
Serotonin
Haplorhini
Homovanillic Acid
Poisons
Punctures
Ether
Spinal Cord
Brain

Keywords

  • (±)-3,4-Methylenedioxymethamphetamine
  • 5-Hydroxyindoleacetic acid
  • Amphetamine
  • Cerebrospinal fluid
  • Neurotoxicity
  • Serotonin

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

5-Hydroxyindoleacetic acid in cerebrospinal fluid reflects serotonergic damage induced by 3,4-methylenedioxymethamphetamine in CNS of non-human primates. / Ricaurte, George; DeLanney, L. E.; Wiener, S. G.; Irwin, I.; Langston, J. W.

In: Brain Research, Vol. 474, No. 2, 06.12.1988, p. 359-363.

Research output: Contribution to journalArticle

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