4-hydroxy-1-(3-pyridyl)-1-butanone-hemoglobin adducts as biomarkers of exposure to tobacco smoke: Validation of a method to be used in multicenter studies

Sunday E. Atawodi, Suzanne Lea, Fredrik Nyberg, Anush Mukeria, Vali Constantinescu, Wolfgang Ahrens, Irene Brueske-Hohlfeld, Cristina Fortes, Paolo Boffetta, Marlin D. Friesen

Research output: Contribution to journalArticle

Abstract

Hemoglobin (Hb) adducts of 4-hydroxy-1-(3-pyridyl)-1-butanone (HPB), a metabolite of two tobacco-specific nitrosamines [4-(methylnitrosamino)-1-(3- pyridyl)-1-butanone and N'-nitrosonornicotine], were measured as biomarkers of exposure to tobacco smoke as part of a study on genetic alterations and susceptibility to lung cancer among nonsmokers. HPB-Hb adducts were measured after collection of RBCs by Ficoll gradient in six collaborating centers, release of HPB by alkaline hydrolysis from Hb, clean-up by solid-phase extraction, and analysis of an electron-capturing derivative by gas chromatography-electron capture mass spectrometry. Prior to analysis of samples from study subjects, the reproducibility of this approach was validated in blood from donors. The coefficient of variation of reproducibility of paired aliquots from five samples ranged from 7 to 25%; the within-sample reproducibilities of four and eight aliquots were 4 and 16%, respectively. The study subjects consisted of 18 smokers and 52 never- smokers. HPB-Hb adduct levels were significantly higher (P = 0.02) in smokers (26 ± 13 fmol HPB/g Hb) than in never-smokers (20 ± 8 fmol HPB/g Hb). There was no difference between sexes. These results suggest that the level of HPB- Hb adducts, measured using a method modified to facilitate use in multicenter studies, can be a useful biomarker of exposure to tobacco smoke.

Original languageEnglish (US)
Pages (from-to)817-821
Number of pages5
JournalCancer Epidemiology Biomarkers and Prevention
Volume7
Issue number9
StatePublished - Sep 1998
Externally publishedYes

Fingerprint

Smoke
Multicenter Studies
Tobacco
Hemoglobins
Biomarkers
N'-nitrosonornicotine
Electrons
Nitrosamines
Ficoll
Solid Phase Extraction
Genetic Predisposition to Disease
4-hydroxy-1-(3-pyridyl)-1-butanone
Blood Donors
Sex Characteristics
Gas Chromatography
Lung Neoplasms
Mass Spectrometry
Hydrolysis

ASJC Scopus subject areas

  • Epidemiology
  • Oncology

Cite this

4-hydroxy-1-(3-pyridyl)-1-butanone-hemoglobin adducts as biomarkers of exposure to tobacco smoke : Validation of a method to be used in multicenter studies. / Atawodi, Sunday E.; Lea, Suzanne; Nyberg, Fredrik; Mukeria, Anush; Constantinescu, Vali; Ahrens, Wolfgang; Brueske-Hohlfeld, Irene; Fortes, Cristina; Boffetta, Paolo; Friesen, Marlin D.

In: Cancer Epidemiology Biomarkers and Prevention, Vol. 7, No. 9, 09.1998, p. 817-821.

Research output: Contribution to journalArticle

Atawodi, SE, Lea, S, Nyberg, F, Mukeria, A, Constantinescu, V, Ahrens, W, Brueske-Hohlfeld, I, Fortes, C, Boffetta, P & Friesen, MD 1998, '4-hydroxy-1-(3-pyridyl)-1-butanone-hemoglobin adducts as biomarkers of exposure to tobacco smoke: Validation of a method to be used in multicenter studies', Cancer Epidemiology Biomarkers and Prevention, vol. 7, no. 9, pp. 817-821.
Atawodi, Sunday E. ; Lea, Suzanne ; Nyberg, Fredrik ; Mukeria, Anush ; Constantinescu, Vali ; Ahrens, Wolfgang ; Brueske-Hohlfeld, Irene ; Fortes, Cristina ; Boffetta, Paolo ; Friesen, Marlin D. / 4-hydroxy-1-(3-pyridyl)-1-butanone-hemoglobin adducts as biomarkers of exposure to tobacco smoke : Validation of a method to be used in multicenter studies. In: Cancer Epidemiology Biomarkers and Prevention. 1998 ; Vol. 7, No. 9. pp. 817-821.
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abstract = "Hemoglobin (Hb) adducts of 4-hydroxy-1-(3-pyridyl)-1-butanone (HPB), a metabolite of two tobacco-specific nitrosamines [4-(methylnitrosamino)-1-(3- pyridyl)-1-butanone and N'-nitrosonornicotine], were measured as biomarkers of exposure to tobacco smoke as part of a study on genetic alterations and susceptibility to lung cancer among nonsmokers. HPB-Hb adducts were measured after collection of RBCs by Ficoll gradient in six collaborating centers, release of HPB by alkaline hydrolysis from Hb, clean-up by solid-phase extraction, and analysis of an electron-capturing derivative by gas chromatography-electron capture mass spectrometry. Prior to analysis of samples from study subjects, the reproducibility of this approach was validated in blood from donors. The coefficient of variation of reproducibility of paired aliquots from five samples ranged from 7 to 25{\%}; the within-sample reproducibilities of four and eight aliquots were 4 and 16{\%}, respectively. The study subjects consisted of 18 smokers and 52 never- smokers. HPB-Hb adduct levels were significantly higher (P = 0.02) in smokers (26 ± 13 fmol HPB/g Hb) than in never-smokers (20 ± 8 fmol HPB/g Hb). There was no difference between sexes. These results suggest that the level of HPB- Hb adducts, measured using a method modified to facilitate use in multicenter studies, can be a useful biomarker of exposure to tobacco smoke.",
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AU - Lea, Suzanne

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AU - Ahrens, Wolfgang

AU - Brueske-Hohlfeld, Irene

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