4-1BB is superior to CD28 costimulation for generating CD8+ cytotoxic lymphocytes for adoptive immunotherapy

Hua Zhang, Kristen M. Snyder, Megan M. Suhoski, Marcela V. Maus, Veena Kapoor, Carl H. June, Crystal L. Mackall

Research output: Contribution to journalArticle

Abstract

Artificial APCs (aAPCs) genetically modified to express selective costimulatory molecules provide a reproducible, cost-effective, and convenient method for polyclonal and Ag-specific expansion of human T cells for adoptive immunotherapy. Among the variety of aAPCs that have been studied, acellular beads expressing anti-CD3/anti-CD28 efficiently expand CD4+ cells, but not CD8+ T cells. Cell-based aAPCs can effectively expand cytolytic CD8+ cells, but optimal costimulatory signals have not been defined. 4-1BB, a costimulatory molecule expressed by a minority of resting CD8+ T cells, is transiently up-regulated by all CD8+ T cells following activation. We compared expansion of human cytolytic CD8 + T cells using cell-based aAPCs providing costimulation via 4-1BB vs CD28. Whereas anti-CD3/anti-CD28 aAPCs mostly expand naive cells, anti-CD3/4-1BBL aAPCs preferentially expand memory cells, resulting in superior enrichment of Ag-reactive T cells which recognize previously primed Ags and efficient expansion of electronically sorted CD8+ populations reactive toward viral or self-Ags. Using HLA-A2-Fc fusion proteins linked to 4-1BBL aAPCs, 3-log expansion of Ag-specific CD8+ CTL was induced over 14 days, whereas similar Ag-specific CD8+ T cell expansion did not occur using HLA-A2-Fc/anti-CD28 aAPCs. Furthermore, when compared with cytolytic T cells expanded using CD28 costimulation, CTL expanded using 4-1BB costimulation mediate enhanced cytolytic capacity due, in part, to NKG2D up-regulation. These results demonstrate that 4-1BB costimulation is essential for expanding memory CD8+ T cells ex vivo and is superior to CD28 costimulation for generating Ag-specific products for adoptive cell therapy.

Original languageEnglish (US)
Pages (from-to)4910-4918
Number of pages9
JournalJournal of Immunology
Volume179
Issue number7
StatePublished - Oct 1 2007
Externally publishedYes

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Adoptive Immunotherapy
Lymphocytes
T-Lymphocytes
Artificial Cells
HLA-A2 Antigen
Cell- and Tissue-Based Therapy
Up-Regulation
Costs and Cost Analysis

ASJC Scopus subject areas

  • Immunology

Cite this

Zhang, H., Snyder, K. M., Suhoski, M. M., Maus, M. V., Kapoor, V., June, C. H., & Mackall, C. L. (2007). 4-1BB is superior to CD28 costimulation for generating CD8+ cytotoxic lymphocytes for adoptive immunotherapy. Journal of Immunology, 179(7), 4910-4918.

4-1BB is superior to CD28 costimulation for generating CD8+ cytotoxic lymphocytes for adoptive immunotherapy. / Zhang, Hua; Snyder, Kristen M.; Suhoski, Megan M.; Maus, Marcela V.; Kapoor, Veena; June, Carl H.; Mackall, Crystal L.

In: Journal of Immunology, Vol. 179, No. 7, 01.10.2007, p. 4910-4918.

Research output: Contribution to journalArticle

Zhang, H, Snyder, KM, Suhoski, MM, Maus, MV, Kapoor, V, June, CH & Mackall, CL 2007, '4-1BB is superior to CD28 costimulation for generating CD8+ cytotoxic lymphocytes for adoptive immunotherapy', Journal of Immunology, vol. 179, no. 7, pp. 4910-4918.
Zhang H, Snyder KM, Suhoski MM, Maus MV, Kapoor V, June CH et al. 4-1BB is superior to CD28 costimulation for generating CD8+ cytotoxic lymphocytes for adoptive immunotherapy. Journal of Immunology. 2007 Oct 1;179(7):4910-4918.
Zhang, Hua ; Snyder, Kristen M. ; Suhoski, Megan M. ; Maus, Marcela V. ; Kapoor, Veena ; June, Carl H. ; Mackall, Crystal L. / 4-1BB is superior to CD28 costimulation for generating CD8+ cytotoxic lymphocytes for adoptive immunotherapy. In: Journal of Immunology. 2007 ; Vol. 179, No. 7. pp. 4910-4918.
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