[3H]opiate binding: anomalous properties in kidney and liver membranes

R. Simantov, S. R. Childers, Solomon H Snyder

Research output: Contribution to journalArticle

Abstract

[3H] Naloxone and [3H] dihydromorphine bind in a saturable fashion and with high affinity to membrane preparations of guinea pig kidney and liver. Binding in guinea pig kidney displays reversed stereospecificity, with pharmacologically inactive dextrallorphan being more potent than the known pharmacologically active levallorphan. Opiate agonists tend to be more potent than their corresponding antagonists in competing for [3H] opiate binding in guinea pig kidney. Unlike brain opiate receptors, in which sodium and manganese selectively decrease and increase, respectively, the binding of [3H] opiate agonists, these ions have no selective effect on the binding of [3H] opiates in guinea pig kidney and liver. The opioid peptides Met-enkephalin and β-endorphin and the opiates etorphine and diprenorphine, which have very high affinity for brain opiate receptors, have negligible effects on [3H] opiate binding in guinea pig kidney.

Original languageEnglish (US)
Pages (from-to)69-76
Number of pages8
JournalMolecular Pharmacology
Volume14
Issue number1
StatePublished - 1978

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Opiate Alkaloids
Guinea Pigs
Kidney
Membranes
Liver
Opioid Receptors
Levallorphan
Dihydromorphine
Diprenorphine
Etorphine
Endorphins
Methionine Enkephalin
Opioid Peptides
Brain
Manganese
Naloxone
Sodium
Ions

ASJC Scopus subject areas

  • Pharmacology

Cite this

[3H]opiate binding : anomalous properties in kidney and liver membranes. / Simantov, R.; Childers, S. R.; Snyder, Solomon H.

In: Molecular Pharmacology, Vol. 14, No. 1, 1978, p. 69-76.

Research output: Contribution to journalArticle

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