TY - JOUR
T1 - [3H]Captopril binding to membrane associated angiotensin converting enzyme
AU - Strittmatter, Stephen M.
AU - Kapiloff, Michael S.
AU - Snyder, Solomon H.
N1 - Funding Information:
Supported by USPHS grants MH-18501, DA-00266, NS-16375, RSA Award UA-00074 to S.H.S., training grant GM-073090 to S.M.S. and a grant of the McKniyht Foundation. We thank Dawn C. Hanks for manuscript preparation.
PY - 1983/5/16
Y1 - 1983/5/16
N2 - [3H]Captopril binding to membrane fractions of rat tissues is saturable and reversible with a KD of 2.4nM. [3H]Captopril binding and angiotensin converting enzyme measured with hippuryl-L-histidine-L-leucine are distributed in parallel between different tissues and brain regions, with highest levels in the choroid plexus, lung and corpus striatum. Captopril, N-(1(S)-carboxy-3-phenyl-propyl)-L-alanyl-L-proline, N-(1(S)-carboxy-3-phenyl-propyl)-L-lysyl-L-proline, teprotide, thiorphan and S-acetylcaptopril each have similar potencies for inhibition of [3H]captopril binding and of angiotensin converting enzyme. These data strongly indicate that [3H]Captopril binding evaluation should help clarify the localization and function of angiotensin converting enzyme and assist in defining pharmacologic actions of captopril.
AB - [3H]Captopril binding to membrane fractions of rat tissues is saturable and reversible with a KD of 2.4nM. [3H]Captopril binding and angiotensin converting enzyme measured with hippuryl-L-histidine-L-leucine are distributed in parallel between different tissues and brain regions, with highest levels in the choroid plexus, lung and corpus striatum. Captopril, N-(1(S)-carboxy-3-phenyl-propyl)-L-alanyl-L-proline, N-(1(S)-carboxy-3-phenyl-propyl)-L-lysyl-L-proline, teprotide, thiorphan and S-acetylcaptopril each have similar potencies for inhibition of [3H]captopril binding and of angiotensin converting enzyme. These data strongly indicate that [3H]Captopril binding evaluation should help clarify the localization and function of angiotensin converting enzyme and assist in defining pharmacologic actions of captopril.
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U2 - 10.1016/0006-291X(83)91721-7
DO - 10.1016/0006-291X(83)91721-7
M3 - Article
C2 - 6303332
AN - SCOPUS:0020646405
SN - 0006-291X
VL - 112
SP - 1027
EP - 1033
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -