Abstract
Determining how signaling dynamics relate to gene expression and cell fate is essential to understanding multicellular development. We present a unified live imaging and lineage analysis method that allows integrated analysis of both techniques in the same mouse embryos. This protocol describes the embryo isolation, confocal imaging, immunofluorescence, and in silico alignment required to connect time-lapse and endpoint measurements. By utilizing different biosensors and fixed readouts, this method allows interrogation of signaling dynamics that specify cell fates in developing embryos. For complete details on the use and execution of this protocol, please refer to Pokrass et al. (2020).
Original language | English (US) |
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Article number | 100446 |
Journal | STAR Protocols |
Volume | 2 |
Issue number | 2 |
DOIs | |
State | Published - Jun 18 2021 |
Keywords
- Cell differentiation
- Microscopy
- Signal transduction
- Single cell
ASJC Scopus subject areas
- General Medicine
- General Biochemistry, Genetics and Molecular Biology
- General Immunology and Microbiology
- General Neuroscience