3D-printing composite polycaprolactone-decellularized bone matrix scaffolds for bone tissue engineering applications

Alexandra N. Rindone, Ethan Nyberg, Warren L. Grayson

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Millions of patients worldwide require bone grafts for treatment of large, critically sized bone defects from conditions such as trauma, cancer, and congenital defects. Tissue engineered (TE) bone grafts have the potential to provide a more effective treatment than current bone grafts since they would restore fully functional bone tissue in large defects. Most bone TE approaches involve a combination of stem cells with porous, biodegradable scaffolds that provide mechanical support and degrade gradually as bone tissue is regenerated by stem cells. 3D-printing is a key technique in bone TE that can be used to fabricate functionalized scaffolds with patient-specific geometry. Using 3D-printing, composite polycaprolactone (PCL) and decellularized bone matrix (DCB) scaffolds can be produced to have the desired mechanical properties, geometry, and osteoinductivity needed for a TE bone graft. This book chapter will describe the protocols for fabricating and characterizing 3D-printed PCL:DCB scaffolds. Moreover, procedures for culturing adipose-derived stem cells (ASCs) in these scaffolds in vitro will be described to demonstrate the osteoinductivity of the scaffolds.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages209-226
Number of pages18
DOIs
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1577
ISSN (Print)1064-3745

Keywords

  • 3D-printing
  • Adipose-derived stem cells
  • Bone tissue engineering
  • Decellularized bone matrix
  • Polycaprolactone

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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