384 hanging drop arrays give excellent Z-factors and allow versatile formation of co-culture spheroids

Amy Y. Hsiao, Yi Chung Tung, Xianggui Qu, Lalit R. Patel, Kenneth J. Pienta, Shuichi Takayama

Research output: Contribution to journalArticlepeer-review

Abstract

We previously reported the development of a simple, user-friendly, and versatile 384 hanging drop array plate for 3D spheroid culture and the importance of utilizing 3D cellular models in anti-cancer drug sensitivity testing. The 384 hanging drop array plate allows for high-throughput capabilities and offers significant improvements over existing 3D spheroid culture methods. To allow for practical 3D cell-based high-throughput screening and enable broader use of the plate, we characterize the robustness of the 384 hanging drop array plate in terms of assay performance and demonstrate the versatility of the plate. We find that the 384 hanging drop array plate performance is robust in fluorescence- and colorimetric-based assays through Z-factor calculations. Finally, we demonstrate different plate capabilities and applications, including: spheroid transfer and retrieval for Janus spheroid formation, sequential addition of cells for concentric layer patterning of different cell types, and culture of a wide variety of cell types.

Original languageEnglish (US)
Pages (from-to)1293-1304
Number of pages12
JournalBiotechnology and bioengineering
Volume109
Issue number5
DOIs
StatePublished - May 2012
Externally publishedYes

Keywords

  • 3D
  • Hanging drop
  • High-throughput
  • Spheroid
  • Z-factor

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Fingerprint Dive into the research topics of '384 hanging drop arrays give excellent Z-factors and allow versatile formation of co-culture spheroids'. Together they form a unique fingerprint.

Cite this