Abstract
Cells that have incorporated 5-bromodeoxyuridine (BrdU) into their DNA become extremely sensitive to photoinduced killing after treatment with the dye, 33258 Hoechst. Under appropriate conditions the dye increases the efficiency of killing by a factor of approximately 104. This enhanced photosensitivity suggests that the combination of BrdU, dye, and light can be used to select conditional lethal mutants.
Original language | English (US) |
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Pages (from-to) | 285-290 |
Number of pages | 6 |
Journal | Somatic Cell Genetics |
Volume | 2 |
Issue number | 3 |
DOIs | |
State | Published - May 1 1976 |
Externally published | Yes |
ASJC Scopus subject areas
- Genetics