33258 Hoechst enhancement of the photosensitivity of bromodeoxyuridine-substituted cells

Gail Stetten, Samuel A. Latt, Richard L. Davidson

Research output: Contribution to journalArticlepeer-review

Abstract

Cells that have incorporated 5-bromodeoxyuridine (BrdU) into their DNA become extremely sensitive to photoinduced killing after treatment with the dye, 33258 Hoechst. Under appropriate conditions the dye increases the efficiency of killing by a factor of approximately 104. This enhanced photosensitivity suggests that the combination of BrdU, dye, and light can be used to select conditional lethal mutants.

Original languageEnglish (US)
Pages (from-to)285-290
Number of pages6
JournalSomatic Cell Genetics
Volume2
Issue number3
DOIs
StatePublished - May 1 1976

ASJC Scopus subject areas

  • Genetics

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