TY - JOUR
T1 - 31P NMR spectroscopy of isolated perfused lungs
AU - Pillai, R. P.
AU - Buescher, P. C.
AU - Pearse, D. B.
AU - Sylvester, J. T.
AU - Eichhorn, G. L.
PY - 1986/6
Y1 - 1986/6
N2 - 31P NMR spectra of isolated blood‐perfused pig lungs were obtained by degassing the lungs in vivo to remove field inhomogeneities caused by air‐tissue interfaces. The spectra show the presence of ATP, phosphodiester, inorganic phosphate, and phosphomonoester, but no phosphocreatine. All the metabolites remained stable for more than 4 h when the lungs were perfused with oxygenated blood. Blood gas tensions, glucose concentration, pH, and temperature were controlled throughout the experiment. During anoxia or isch‐emia, ATP and intracellular pH declined and Pi increased but returned to control levels during subsequent normoxia or reperfusion. These results demonstrate the applicability of NMR spectroscopy to isolated perfused lungs, enabling studies of metabolic processes in normal and pathologic lungs, as well as establishment of optimal conditions for lung preservation for transplantation. © 1986 Academic Press, Inc.
AB - 31P NMR spectra of isolated blood‐perfused pig lungs were obtained by degassing the lungs in vivo to remove field inhomogeneities caused by air‐tissue interfaces. The spectra show the presence of ATP, phosphodiester, inorganic phosphate, and phosphomonoester, but no phosphocreatine. All the metabolites remained stable for more than 4 h when the lungs were perfused with oxygenated blood. Blood gas tensions, glucose concentration, pH, and temperature were controlled throughout the experiment. During anoxia or isch‐emia, ATP and intracellular pH declined and Pi increased but returned to control levels during subsequent normoxia or reperfusion. These results demonstrate the applicability of NMR spectroscopy to isolated perfused lungs, enabling studies of metabolic processes in normal and pathologic lungs, as well as establishment of optimal conditions for lung preservation for transplantation. © 1986 Academic Press, Inc.
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U2 - 10.1002/mrm.1910030313
DO - 10.1002/mrm.1910030313
M3 - Article
C2 - 3724427
AN - SCOPUS:0022555964
SN - 0740-3194
VL - 3
SP - 467
EP - 472
JO - Magnetic resonance in medicine
JF - Magnetic resonance in medicine
IS - 3
ER -