3-Hydroxybutyrate regulates energy metabolism and induces BDNF expression in cerebral cortical neurons

Krisztina Marosi, Sang Woo Kim, Keelin Moehl, Morten Scheibye-Knudsen, Aiwu Cheng, Roy Cutler, Simonetta Camandola, Mark P. Mattson

Research output: Contribution to journalArticle

Abstract

During fasting and vigorous exercise, a shift of brain cell energy substrate utilization from glucose to the ketone 3-hydroxybutyrate (3OHB) occurs. Studies have shown that 3OHB can protect neurons against excitotoxicity and oxidative stress, but the underlying mechanisms remain unclear. Neurons maintained in the presence of 3OHB exhibited increased oxygen consumption and ATP production, and an elevated NAD+/NADH ratio. We found that 3OHB metabolism increases mitochondrial respiration which drives changes in expression of brain-derived neurotrophic factor (BDNF) in cultured cerebral cortical neurons. The mechanism by which 3OHB induces Bdnf gene expression involves generation of reactive oxygen species, activation of the transcription factor NF-κB, and activity of the histone acetyltransferase p300/EP300. Because BDNF plays important roles in synaptic plasticity and neuronal stress resistance, our findings suggest cellular signaling mechanisms by which 3OHB may mediate adaptive responses of neurons to fasting, exercise, and ketogenic diets. (Figure presented.).

Original languageEnglish (US)
Pages (from-to)769-781
Number of pages13
JournalJournal of Neurochemistry
Volume139
Issue number5
DOIs
StatePublished - Dec 1 2016

Fingerprint

3-Hydroxybutyric Acid
Brain-Derived Neurotrophic Factor
Energy Metabolism
Neurons
NAD
Fasting
Ketogenic Diet
Cell signaling
Histone Acetyltransferases
Neuronal Plasticity
Oxidative stress
Nutrition
Ketones
Metabolism
Gene expression
Oxygen Consumption
Plasticity
Reactive Oxygen Species
Brain
Respiration

Keywords

  • bdnf
  • ketone
  • mitochondria
  • nf-kb
  • p300

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Marosi, K., Kim, S. W., Moehl, K., Scheibye-Knudsen, M., Cheng, A., Cutler, R., ... Mattson, M. P. (2016). 3-Hydroxybutyrate regulates energy metabolism and induces BDNF expression in cerebral cortical neurons. Journal of Neurochemistry, 139(5), 769-781. https://doi.org/10.1111/jnc.13868

3-Hydroxybutyrate regulates energy metabolism and induces BDNF expression in cerebral cortical neurons. / Marosi, Krisztina; Kim, Sang Woo; Moehl, Keelin; Scheibye-Knudsen, Morten; Cheng, Aiwu; Cutler, Roy; Camandola, Simonetta; Mattson, Mark P.

In: Journal of Neurochemistry, Vol. 139, No. 5, 01.12.2016, p. 769-781.

Research output: Contribution to journalArticle

Marosi, K, Kim, SW, Moehl, K, Scheibye-Knudsen, M, Cheng, A, Cutler, R, Camandola, S & Mattson, MP 2016, '3-Hydroxybutyrate regulates energy metabolism and induces BDNF expression in cerebral cortical neurons', Journal of Neurochemistry, vol. 139, no. 5, pp. 769-781. https://doi.org/10.1111/jnc.13868
Marosi, Krisztina ; Kim, Sang Woo ; Moehl, Keelin ; Scheibye-Knudsen, Morten ; Cheng, Aiwu ; Cutler, Roy ; Camandola, Simonetta ; Mattson, Mark P. / 3-Hydroxybutyrate regulates energy metabolism and induces BDNF expression in cerebral cortical neurons. In: Journal of Neurochemistry. 2016 ; Vol. 139, No. 5. pp. 769-781.
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