(3-Aminopropyl)-4-methylpiperazine end-capped poly(1,4-butanediol diacrylate-co-4-amino-1-butanol)-based multilayer films for gene delivery

Cuicui Li, Stephany Tzeng, Liane E. Tellier, Jordan Green

Research output: Contribution to journalArticle

Abstract

Biodegradable polyelectrolyte surfaces for gene delivery were created through electrospinning of biodegradable polycations combined with iterative solution-based multilayer coating. Poly(β-amino ester) (PBAE) poly(1,4-butanediol diacrylate-co-4-amino-1-butanol) end-capped with 1-(3-aminopropyl)-4-methylpiperazine was utilized because of its ability to electrostatically interact with anionic molecules like DNA, its biodegradability, and its low cytotoxicity. A new DNA release system was developed for sustained release of DNA over 24 h, accompanied by high exogenous gene expression in primary human glioblastoma (GB) cells. Electrospinning a different PBAE, poly(1,4-butanediol diacrylate-co-4,4′- trimethylenedipiperidine), and its combination with polyelectrolyte 1-(3-aminopropyl)-4-methylpiperazine end-capped poly(1,4-butanediol diacrylate-co-4-amino-1-butanol)-based multilayers are promising for DNA release and intracellular delivery from a surface.

Original languageEnglish (US)
Pages (from-to)5947-5953
Number of pages7
JournalACS Applied Materials and Interfaces
Volume5
Issue number13
DOIs
Publication statusPublished - Jul 10 2013

    Fingerprint

Keywords

  • biodegradable
  • electrospinning
  • gene delivery
  • layer-by-layer
  • poly(β-amino ester)

ASJC Scopus subject areas

  • Materials Science(all)
  • Medicine(all)

Cite this