2,5-Di(tert-butyl)-1,4-benzohydroquinone - a novel inhibitor of liver microsomal Ca2+ sequestration

Gregory A. Moore, David J. McConkey, Georges E.N. Kass, Peter J. O'Brien, Sten Orrenius

Research output: Contribution to journalArticle

Abstract

Treatment of rat liver microsomes with 2,5-di(tert-butyl)-1,4-benzohydroquinone caused a dose-related inhibition (Ki{reversed tilde equals}1 μM) of ATP-dependent Ca2+ sequestration. This was paralleled by a similar impairment of the microsomal Ca2+ -stimulated ATPase activity. In contrast, the hydroquinone failed to induce Ca2+ release from Ca2+ -loaded liver mitochondria (supplied with ATP), and inhibited neither the mitochondrial F1F0-ATPase nor the Ca2+ -stimulated ATPase activity of the hepatic plasma membrane fraction. The inhibition of microsomal Ca2+ sequestration was not associated with any apparent alteration of membrane permeability or loss of other microsomal enzyme activities or modification of microsomal protein thiols. These findings suggest that 2,5-di(tert-butyl)-1,4-benzohydroquinone is a potent and selective inhibitor of liver microsomal Ca2+ sequestration which may be a useful tool in studies of Ca2+ fluxes in intact cells and tissues.

Original languageEnglish (US)
Pages (from-to)331-336
Number of pages6
JournalFEBS Letters
Volume224
Issue number2
DOIs
StatePublished - Nov 30 1987
Externally publishedYes

Keywords

  • (Ca + Mg)-ATPase
  • (Rat liver)
  • 2,5-Di(tert-butyl)-1,4-benzohydroquinone
  • Ca sequestration
  • Microsome

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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