TY - JOUR
T1 - 2,3,7,8-Tetrachlorodibenzo-p-dioin kills immature thymoytes by Ca2+-mediated endonudease activation
AU - McConkey, David J.
AU - Hartzell, Pia
AU - Duddy, Steven K.
AU - Håkansson, Helen
AU - Orrenius, Sten
PY - 1988
Y1 - 1988
N2 - Suspensions of thymocytes from young rats were incubated with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), which resulted in a sustained increase in cytosolic free Ca2+ concentration followed by DNA fragmentation and loss of cell viability. Both the Ca2+ increase and DNA fragmentation were pervented in cells treated with the inhibitor of protein synthesis, cycloheximide, and DNA fagmentation and cell killing were not detected when cells were incubated in a "Ca2+-free" medium or pretreated with high concentrations of the calcium probe, quin-2 tetraacetoxymethyl ester. These results indicate that TCDD can kill immature thymocytes by initiating a suicide process similar to that previously described for glucocorticoid hormones.
AB - Suspensions of thymocytes from young rats were incubated with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), which resulted in a sustained increase in cytosolic free Ca2+ concentration followed by DNA fragmentation and loss of cell viability. Both the Ca2+ increase and DNA fragmentation were pervented in cells treated with the inhibitor of protein synthesis, cycloheximide, and DNA fagmentation and cell killing were not detected when cells were incubated in a "Ca2+-free" medium or pretreated with high concentrations of the calcium probe, quin-2 tetraacetoxymethyl ester. These results indicate that TCDD can kill immature thymocytes by initiating a suicide process similar to that previously described for glucocorticoid hormones.
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U2 - 10.1126/science.3262923
DO - 10.1126/science.3262923
M3 - Article
C2 - 3262923
AN - SCOPUS:0024286320
SN - 0036-8075
VL - 242
SP - 256
EP - 259
JO - Science
JF - Science
IS - 4876
ER -