2,3,7,8-Tetrachlorodibenzo-p-dioin kills immature thymoytes by Ca2+-mediated endonudease activation

David McConkey, Pia Hartzell, Steven K. Duddy, Helen Håkansson, Sten Orrenius

Research output: Contribution to journalArticle

Abstract

Suspensions of thymocytes from young rats were incubated with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), which resulted in a sustained increase in cytosolic free Ca2+ concentration followed by DNA fragmentation and loss of cell viability. Both the Ca2+ increase and DNA fragmentation were pervented in cells treated with the inhibitor of protein synthesis, cycloheximide, and DNA fagmentation and cell killing were not detected when cells were incubated in a "Ca2+-free" medium or pretreated with high concentrations of the calcium probe, quin-2 tetraacetoxymethyl ester. These results indicate that TCDD can kill immature thymocytes by initiating a suicide process similar to that previously described for glucocorticoid hormones.

Original languageEnglish (US)
Pages (from-to)256-259
Number of pages4
JournalScience
Volume242
Issue number4876
StatePublished - 1988
Externally publishedYes

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DNA Fragmentation
Thymocytes
Protein Synthesis Inhibitors
Cycloheximide
Suicide
Glucocorticoids
Cell Survival
Suspensions
Esters
Hormones
Calcium
DNA
Polychlorinated Dibenzodioxins
Quin2

ASJC Scopus subject areas

  • General

Cite this

McConkey, D., Hartzell, P., Duddy, S. K., Håkansson, H., & Orrenius, S. (1988). 2,3,7,8-Tetrachlorodibenzo-p-dioin kills immature thymoytes by Ca2+-mediated endonudease activation. Science, 242(4876), 256-259.

2,3,7,8-Tetrachlorodibenzo-p-dioin kills immature thymoytes by Ca2+-mediated endonudease activation. / McConkey, David; Hartzell, Pia; Duddy, Steven K.; Håkansson, Helen; Orrenius, Sten.

In: Science, Vol. 242, No. 4876, 1988, p. 256-259.

Research output: Contribution to journalArticle

McConkey, D, Hartzell, P, Duddy, SK, Håkansson, H & Orrenius, S 1988, '2,3,7,8-Tetrachlorodibenzo-p-dioin kills immature thymoytes by Ca2+-mediated endonudease activation', Science, vol. 242, no. 4876, pp. 256-259.
McConkey, David ; Hartzell, Pia ; Duddy, Steven K. ; Håkansson, Helen ; Orrenius, Sten. / 2,3,7,8-Tetrachlorodibenzo-p-dioin kills immature thymoytes by Ca2+-mediated endonudease activation. In: Science. 1988 ; Vol. 242, No. 4876. pp. 256-259.
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