2',3'-cyclic nucleotide 3'-phosphodiesterase: A novel candidate autoantigen in demyelinating diseases

Martin Rösener; Paolo A. Muraro; Adrienne Riethmüller; Matthias Kalbus; Gregor Sappler; R. J. Thompson; Rudolf Lichtenfels; Norbert Sommer; Henry F. McFarland; Roland Martin

doi:10.1016/S0165-5728(96)00230-5

2',3'-cyclic nucleotide 3'-phosphodiesterase: A novel candidate autoantigen in demyelinating diseases

Martin Rösener, Paolo A. Muraro, Adrienne Riethmüller, Matthias Kalbus, Gregor Sappler, R. J. Thompson, Rudolf Lichtenfels, Norbert Sommer, Henry F. McFarland, Roland Martin

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

Autoaggressive T-cells specific for myelin proteins like proteolipid protein (PLP) and myelin basic protein (MBP) are thought to play a major role in the pathogenesis of demyelinating diseases of the central nervous system (CNS). 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) is the third most abundant myelin protein in the CNS. Due to lack of supply with enough CNPase of sufficient purity its immunologic properties have not been studied yet. We subcloned a human CNPase cDNA and expressed human recombinant CNPase (rh-CNPase) in E. coli. Purification of the protein was achieved by Ni²⁺-chelating chromatography. Furthermore we describe for the first time several rh-CNPase specific T-cell lines from a multiple sclerosis patient and a healthy control.

Original language	English (US)
Pages (from-to)	28-34
Number of pages	7
Journal	Journal of Neuroimmunology
Volume	75
Issue number	1-2
DOIs	https://doi.org/10.1016/S0165-5728(96)00230-5
State	Published - May 1997
Externally published	Yes

Keywords

2',3'-cyclic nucleotide 3'-phosphodiesterase
multiple sclerosis
T-cell-response

ASJC Scopus subject areas

Immunology
Clinical Neurology
Immunology and Allergy
Neurology

Access to Document

10.1016/S0165-5728(96)00230-5

Cite this

@article{17c029176b3046d3b46870beec3268da,

title = "2',3'-cyclic nucleotide 3'-phosphodiesterase: A novel candidate autoantigen in demyelinating diseases",

abstract = "Autoaggressive T-cells specific for myelin proteins like proteolipid protein (PLP) and myelin basic protein (MBP) are thought to play a major role in the pathogenesis of demyelinating diseases of the central nervous system (CNS). 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) is the third most abundant myelin protein in the CNS. Due to lack of supply with enough CNPase of sufficient purity its immunologic properties have not been studied yet. We subcloned a human CNPase cDNA and expressed human recombinant CNPase (rh-CNPase) in E. coli. Purification of the protein was achieved by Ni2+-chelating chromatography. Furthermore we describe for the first time several rh-CNPase specific T-cell lines from a multiple sclerosis patient and a healthy control.",

keywords = "2',3'-cyclic nucleotide 3'-phosphodiesterase, multiple sclerosis, T-cell-response",

author = "Martin R{\"o}sener and Muraro, {Paolo A.} and Adrienne Riethm{\"u}ller and Matthias Kalbus and Gregor Sappler and Thompson, {R. J.} and Rudolf Lichtenfels and Norbert Sommer and McFarland, {Henry F.} and Roland Martin",

year = "1997",

month = may,

doi = "10.1016/S0165-5728(96)00230-5",

language = "English (US)",

volume = "75",

pages = "28--34",

journal = "Journal of Neuroimmunology",

issn = "0165-5728",

publisher = "Elsevier",

number = "1-2",

}

TY - JOUR

T1 - 2',3'-cyclic nucleotide 3'-phosphodiesterase

T2 - A novel candidate autoantigen in demyelinating diseases

AU - Rösener, Martin

AU - Muraro, Paolo A.

AU - Riethmüller, Adrienne

AU - Kalbus, Matthias

AU - Sappler, Gregor

AU - Thompson, R. J.

AU - Lichtenfels, Rudolf

AU - Sommer, Norbert

AU - McFarland, Henry F.

AU - Martin, Roland

PY - 1997/5

Y1 - 1997/5

N2 - Autoaggressive T-cells specific for myelin proteins like proteolipid protein (PLP) and myelin basic protein (MBP) are thought to play a major role in the pathogenesis of demyelinating diseases of the central nervous system (CNS). 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) is the third most abundant myelin protein in the CNS. Due to lack of supply with enough CNPase of sufficient purity its immunologic properties have not been studied yet. We subcloned a human CNPase cDNA and expressed human recombinant CNPase (rh-CNPase) in E. coli. Purification of the protein was achieved by Ni2+-chelating chromatography. Furthermore we describe for the first time several rh-CNPase specific T-cell lines from a multiple sclerosis patient and a healthy control.

AB - Autoaggressive T-cells specific for myelin proteins like proteolipid protein (PLP) and myelin basic protein (MBP) are thought to play a major role in the pathogenesis of demyelinating diseases of the central nervous system (CNS). 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) is the third most abundant myelin protein in the CNS. Due to lack of supply with enough CNPase of sufficient purity its immunologic properties have not been studied yet. We subcloned a human CNPase cDNA and expressed human recombinant CNPase (rh-CNPase) in E. coli. Purification of the protein was achieved by Ni2+-chelating chromatography. Furthermore we describe for the first time several rh-CNPase specific T-cell lines from a multiple sclerosis patient and a healthy control.

KW - 2',3'-cyclic nucleotide 3'-phosphodiesterase

KW - multiple sclerosis

KW - T-cell-response

UR - http://www.scopus.com/inward/record.url?scp=0343487828&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0343487828&partnerID=8YFLogxK

U2 - 10.1016/S0165-5728(96)00230-5

DO - 10.1016/S0165-5728(96)00230-5

M3 - Article

C2 - 9143234

AN - SCOPUS:0343487828

SN - 0165-5728

VL - 75

SP - 28

EP - 34

JO - Journal of Neuroimmunology

JF - Journal of Neuroimmunology

IS - 1-2

ER -

2',3'-cyclic nucleotide 3'-phosphodiesterase: A novel candidate autoantigen in demyelinating diseases

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this