2-sulfotrehalose, a novel osmolyte in haloalkaliphilic archaea

Deana Desmarais, Peter E. Jablonski, Neal S. Fedarko, Mary F. Roberts

Research output: Contribution to journalArticlepeer-review

61 Scopus citations


A novel 1→1 α-linked glucose disaccharide with sulfate at C-2 of one of the glucose moieties, 1-(2-O-sulfo-α-D-glucopyranosyl)-α-D- glycopyranose, was found to be the major organic solute accumulated by a Natronococcus sp. and several Natronobacterium species. The concentration of this novel disaccharide, termed sulfotrehalose, increased with increasing concentrations of external NaCl, behavior consistent with its identity as an osmolyte. A variety of noncharged disaccharides (trehalose, sucrose, cellobiose, and maltose) were added to the growth medium to see if they could suppress synthesis and accumulation of sulfotrehalose. Sucrose was the most effective in suppressing biosynthesis and accumulation of sulfotrehalose, with levels as low as 0.1 mM being able to significantly replace the novel charged osmolyte. Other common osmolytes (glycine betaine, glutamate, and proline) were not accumulated or used for osmotic balance in place of the sulfotrehalose by the halophilic archaeons.

Original languageEnglish (US)
Pages (from-to)3146-3153
Number of pages8
JournalJournal of bacteriology
Issue number10
StatePublished - May 1997

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology


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