2-sulfotrehalose, a novel osmolyte in haloalkaliphilic archaea

Deana Desmarais, Peter E. Jablonski, Neal S Fedarko, Mary F. Roberts

Research output: Contribution to journalArticle

Abstract

A novel 1→1 α-linked glucose disaccharide with sulfate at C-2 of one of the glucose moieties, 1-(2-O-sulfo-α-D-glucopyranosyl)-α-D- glycopyranose, was found to be the major organic solute accumulated by a Natronococcus sp. and several Natronobacterium species. The concentration of this novel disaccharide, termed sulfotrehalose, increased with increasing concentrations of external NaCl, behavior consistent with its identity as an osmolyte. A variety of noncharged disaccharides (trehalose, sucrose, cellobiose, and maltose) were added to the growth medium to see if they could suppress synthesis and accumulation of sulfotrehalose. Sucrose was the most effective in suppressing biosynthesis and accumulation of sulfotrehalose, with levels as low as 0.1 mM being able to significantly replace the novel charged osmolyte. Other common osmolytes (glycine betaine, glutamate, and proline) were not accumulated or used for osmotic balance in place of the sulfotrehalose by the halophilic archaeons.

Original languageEnglish (US)
Pages (from-to)3146-3153
Number of pages8
JournalJournal of Bacteriology
Volume179
Issue number10
StatePublished - May 1997

Fingerprint

Disaccharides
Archaea
Natronococcus
Sucrose
Natronobacterium
Cellobiose
Glucose
Betaine
Trehalose
Maltose
Proline
Sulfates
Glutamic Acid
Growth

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Desmarais, D., Jablonski, P. E., Fedarko, N. S., & Roberts, M. F. (1997). 2-sulfotrehalose, a novel osmolyte in haloalkaliphilic archaea. Journal of Bacteriology, 179(10), 3146-3153.

2-sulfotrehalose, a novel osmolyte in haloalkaliphilic archaea. / Desmarais, Deana; Jablonski, Peter E.; Fedarko, Neal S; Roberts, Mary F.

In: Journal of Bacteriology, Vol. 179, No. 10, 05.1997, p. 3146-3153.

Research output: Contribution to journalArticle

Desmarais, D, Jablonski, PE, Fedarko, NS & Roberts, MF 1997, '2-sulfotrehalose, a novel osmolyte in haloalkaliphilic archaea', Journal of Bacteriology, vol. 179, no. 10, pp. 3146-3153.
Desmarais D, Jablonski PE, Fedarko NS, Roberts MF. 2-sulfotrehalose, a novel osmolyte in haloalkaliphilic archaea. Journal of Bacteriology. 1997 May;179(10):3146-3153.
Desmarais, Deana ; Jablonski, Peter E. ; Fedarko, Neal S ; Roberts, Mary F. / 2-sulfotrehalose, a novel osmolyte in haloalkaliphilic archaea. In: Journal of Bacteriology. 1997 ; Vol. 179, No. 10. pp. 3146-3153.
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