2-Methoxyestradiol, an endogenous metabolite of 17β-estradiol, inhibits adipocyte proliferation

Catalina Picó; Pere Puigserver; Paula Oliver; Andren Palou

2-Methoxyestradiol, an endogenous metabolite of 17β-estradiol, inhibits adipocyte proliferation

Catalina Picó, Pere Puigserver, Paula Oliver, Andren Palou

Research output: Contribution to journal › Article › peer-review

Abstract

The effects of 2-methoxyestradiol (2ME), a naturally occurring mammalian metabolite of 17β-estradiol, on adipocyte growth has been investigated in mouse brown adipose tissue precursor cells developed in primary culture. 2ME inhibits brown adipocyte proliferation in a dose-response manner (IC50 = 1.7 x 10^-6 for DNA synthesis), with much higher potency than its hormone precursor 17β-estradiol, and cells acquire the typical differentiated morphology - more round with a higher content of triglycerides. 2ME causes similar effects in the immortal brown adipocyte tumor-derived hibernoma cell line HIB 1B and the immortal 3T3-F442A white adipocyte line. These findings suggest a possible role for 2ME in adipocyte proliferation, and probably in the differentiation process, entering the cells in the adipogenic program.

Original language	English (US)
Pages (from-to)	1-7
Number of pages	7
Journal	Molecular and Cellular Biochemistry
Volume	189
Issue number	1-2
State	Published - 1998
Externally published	Yes

Keywords

2-Methoxyestradiol
Adipocytes
Cell proliferation
Cultured brown adipocytes

ASJC Scopus subject areas

Clinical Biochemistry
Molecular Biology
Genetics
Cell Biology

Cite this

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title = "2-Methoxyestradiol, an endogenous metabolite of 17β-estradiol, inhibits adipocyte proliferation",

abstract = "The effects of 2-methoxyestradiol (2ME), a naturally occurring mammalian metabolite of 17β-estradiol, on adipocyte growth has been investigated in mouse brown adipose tissue precursor cells developed in primary culture. 2ME inhibits brown adipocyte proliferation in a dose-response manner (IC50 = 1.7 x 10-6 for DNA synthesis), with much higher potency than its hormone precursor 17β-estradiol, and cells acquire the typical differentiated morphology - more round with a higher content of triglycerides. 2ME causes similar effects in the immortal brown adipocyte tumor-derived hibernoma cell line HIB 1B and the immortal 3T3-F442A white adipocyte line. These findings suggest a possible role for 2ME in adipocyte proliferation, and probably in the differentiation process, entering the cells in the adipogenic program.",

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AU - Picó, Catalina

AU - Puigserver, Pere

AU - Oliver, Paula

AU - Palou, Andren

PY - 1998

Y1 - 1998

N2 - The effects of 2-methoxyestradiol (2ME), a naturally occurring mammalian metabolite of 17β-estradiol, on adipocyte growth has been investigated in mouse brown adipose tissue precursor cells developed in primary culture. 2ME inhibits brown adipocyte proliferation in a dose-response manner (IC50 = 1.7 x 10-6 for DNA synthesis), with much higher potency than its hormone precursor 17β-estradiol, and cells acquire the typical differentiated morphology - more round with a higher content of triglycerides. 2ME causes similar effects in the immortal brown adipocyte tumor-derived hibernoma cell line HIB 1B and the immortal 3T3-F442A white adipocyte line. These findings suggest a possible role for 2ME in adipocyte proliferation, and probably in the differentiation process, entering the cells in the adipogenic program.

AB - The effects of 2-methoxyestradiol (2ME), a naturally occurring mammalian metabolite of 17β-estradiol, on adipocyte growth has been investigated in mouse brown adipose tissue precursor cells developed in primary culture. 2ME inhibits brown adipocyte proliferation in a dose-response manner (IC50 = 1.7 x 10-6 for DNA synthesis), with much higher potency than its hormone precursor 17β-estradiol, and cells acquire the typical differentiated morphology - more round with a higher content of triglycerides. 2ME causes similar effects in the immortal brown adipocyte tumor-derived hibernoma cell line HIB 1B and the immortal 3T3-F442A white adipocyte line. These findings suggest a possible role for 2ME in adipocyte proliferation, and probably in the differentiation process, entering the cells in the adipogenic program.

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KW - Cultured brown adipocytes

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2-Methoxyestradiol, an endogenous metabolite of 17β-estradiol, inhibits adipocyte proliferation

Abstract

Keywords

ASJC Scopus subject areas

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