Abstract
The effects of 2-methoxyestradiol (2ME), a naturally occurring mammalian metabolite of 17β-estradiol, on adipocyte growth has been investigated in mouse brown adipose tissue precursor cells developed in primary culture. 2ME inhibits brown adipocyte proliferation in a dose-response manner (IC50 = 1.7 x 10-6 for DNA synthesis), with much higher potency than its hormone precursor 17β-estradiol, and cells acquire the typical differentiated morphology - more round with a higher content of triglycerides. 2ME causes similar effects in the immortal brown adipocyte tumor-derived hibernoma cell line HIB 1B and the immortal 3T3-F442A white adipocyte line. These findings suggest a possible role for 2ME in adipocyte proliferation, and probably in the differentiation process, entering the cells in the adipogenic program.
Original language | English (US) |
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Pages (from-to) | 1-7 |
Number of pages | 7 |
Journal | Molecular and Cellular Biochemistry |
Volume | 189 |
Issue number | 1-2 |
State | Published - 1998 |
Externally published | Yes |
Keywords
- 2-Methoxyestradiol
- Adipocytes
- Cell proliferation
- Cultured brown adipocytes
ASJC Scopus subject areas
- Clinical Biochemistry
- Molecular Biology
- Genetics
- Cell Biology