1H NMR spectroscopy in the diagnosis of Klebsiella pneumoniae-induced urinary tract infection

Ashish Gupta, Mayank Dwivedi, G. A Nagana Gowda, A. A. Mahdi, Amita Jain, Archana Ayyagari, Raja Roy, M. Bhandari, C. L. Khetrapal

Research output: Contribution to journalArticle

Abstract

The 1H NMR spectroscopic method is suggested and its utility is demonstrated for the diagnosis of Klebsiella pneumoniae (K. pneumoniae) in urinary tract infection (UTI). K. pneumoniae have the specific property of metabolizing glycerol to 1,3-propanediol (1,3-PD), acetate, ethanol and succinate. The quantity of 1,3-PD produced correlates well with the viable bacterial count. Other common bacteria causing UTI (except for Citrobacter frundii), such as Escherichia coli (E. coli), Pseudomonas aeruginosa (P. aeruginosa), Enterobacter aerogenes, Acinetobacter baumanii, Proteus mirabilis, Enterococcus faecalis, Streptococcus gp B and Staphylococcus aureus do not metabolize glycerol under similar conditions. Citrobacter frundii (C. frundii) also gives the same NMR results but is easily differentiated as being motile on direct microscopic examination of urine and it is not common nosocomial infectious agent in urinary tract infection. The method provides a single-step documentation of K. pneumoniae (and C. frundii) qualitatively as well as quantitatively. Out of the total 614 subjects considered, clinical diagnosis of UTI was obtained in 516 cases (84%). The NMR-based screening had a sensitivity of 90%, a specificity of 100% and a false negativity of 10% relative to the conventional quantitative culture method. In the present authors' experience, the results of NMR spectroscopy based screening show a very good correlation with the diagnosis of urinary tract infected patients.

Original languageEnglish (US)
Pages (from-to)1055-1061
Number of pages7
JournalNMR in Biomedicine
Volume19
Issue number8
DOIs
StatePublished - Dec 2006
Externally publishedYes

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Klebsiella pneumoniae
Citrobacter
Urinary Tract Infections
Nuclear magnetic resonance spectroscopy
Magnetic Resonance Spectroscopy
Nuclear magnetic resonance
Glycerol
Screening
Succinic Acid
Enterobacter aerogenes
Proteus mirabilis
Escherichia coli
Acinetobacter
Bacterial Load
Enterococcus faecalis
Bacteria
Microscopic examination
Acetates
Ethanol
Streptococcus

Keywords

  • Diagnosis
  • Infection
  • Klebsiella pneumoniae
  • Magnetic resonance
  • Proton

ASJC Scopus subject areas

  • Spectroscopy
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Biophysics

Cite this

Gupta, A., Dwivedi, M., Gowda, G. A. N., Mahdi, A. A., Jain, A., Ayyagari, A., ... Khetrapal, C. L. (2006). 1H NMR spectroscopy in the diagnosis of Klebsiella pneumoniae-induced urinary tract infection. NMR in Biomedicine, 19(8), 1055-1061. https://doi.org/10.1002/nbm.1078

1H NMR spectroscopy in the diagnosis of Klebsiella pneumoniae-induced urinary tract infection. / Gupta, Ashish; Dwivedi, Mayank; Gowda, G. A Nagana; Mahdi, A. A.; Jain, Amita; Ayyagari, Archana; Roy, Raja; Bhandari, M.; Khetrapal, C. L.

In: NMR in Biomedicine, Vol. 19, No. 8, 12.2006, p. 1055-1061.

Research output: Contribution to journalArticle

Gupta, A, Dwivedi, M, Gowda, GAN, Mahdi, AA, Jain, A, Ayyagari, A, Roy, R, Bhandari, M & Khetrapal, CL 2006, '1H NMR spectroscopy in the diagnosis of Klebsiella pneumoniae-induced urinary tract infection', NMR in Biomedicine, vol. 19, no. 8, pp. 1055-1061. https://doi.org/10.1002/nbm.1078
Gupta A, Dwivedi M, Gowda GAN, Mahdi AA, Jain A, Ayyagari A et al. 1H NMR spectroscopy in the diagnosis of Klebsiella pneumoniae-induced urinary tract infection. NMR in Biomedicine. 2006 Dec;19(8):1055-1061. https://doi.org/10.1002/nbm.1078
Gupta, Ashish ; Dwivedi, Mayank ; Gowda, G. A Nagana ; Mahdi, A. A. ; Jain, Amita ; Ayyagari, Archana ; Roy, Raja ; Bhandari, M. ; Khetrapal, C. L. / 1H NMR spectroscopy in the diagnosis of Klebsiella pneumoniae-induced urinary tract infection. In: NMR in Biomedicine. 2006 ; Vol. 19, No. 8. pp. 1055-1061.
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