18S ribosomal DNA-based PCR for diagnosis of Trichomonas vaginalis

Holger Mayta, Robert H. Gilman, Maritza M. Calderon, Aren Gottlieb, Giselle Soto, Iskra Tuero, Sixto Sanchez, Aldo Vivar

Research output: Contribution to journalArticle

Abstract

Trichomonas vaginalis remains the most common sexually transmitted parasite in the world and is considered a major risk factor in the transmission of the human immunodeficiency virus. A PCR technique using primers targeting a specific region of the 18S rRNA gene of T. vaginalis was developed. The PCR test was standardized using 15 reference strains, giving a single product of 312 bp in all strains. No amplification was observed when DNA from related organisms or human DNA was used as a target. The test was evaluated on 372 vaginal swab specimens and 361 urine samples from women attending infertility and obstetric clinics at two separate hospitals in Lima, Peru. Compared to T. vaginalis culture, the overall sensitivity and specificity of PCR of vaginal swab samples was 100% and 98%, respectively. The PCR of urine samples was 100% sensitive and 99.7% specific compared to culture of vaginal swab, but the sensitivity drops to 83.3% when compared to PCR of vaginal swabs. All culture-positive samples were found to be positive by PCR in either urine or vaginal secretion. None of the PCR-negative samples were positive by culture. The origin of the amplification was confirmed by digestion of PCR products with HaeIII. This PCR assay, which is easy to perform and has a high sensitivity and specificity, should be useful for routine diagnosis of T. vaginalis infection.

Original languageEnglish (US)
Pages (from-to)2683-2687
Number of pages5
JournalJournal of clinical microbiology
Volume38
Issue number7
StatePublished - Aug 7 2000

ASJC Scopus subject areas

  • Microbiology (medical)

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    Mayta, H., Gilman, R. H., Calderon, M. M., Gottlieb, A., Soto, G., Tuero, I., Sanchez, S., & Vivar, A. (2000). 18S ribosomal DNA-based PCR for diagnosis of Trichomonas vaginalis. Journal of clinical microbiology, 38(7), 2683-2687.