18F-fluorodeoxyglucose uptake and tumor hypoxia

Revisit 18F-fluorodeoxyglucose in oncology application

Xiao Feng Li, Yang Du, Yuanyuan Ma, Gregory C. Postel, Ali Civelek

Research output: Contribution to journalArticle

Abstract

This study revisited 18F-fluorodeoxyglucose (18F-FDG) uptake and its relationship to hypoxia in various tumor models. METHODS: We generated peritoneal carcinomatosis and subcutaneous xenografts of colorectal cancer HT29, breast cancer MDA-MB-231, and non-small cell lung cancer A549 cell lines in nude mice. The partial oxygen pressure (pO2) of ascites fluid was measured. 18F-FDG accumulation detected by digital autoradiography was related to tumor hypoxia visualized by pimonidazole binding and glucose transporter-1 (GLUT-1) in frozen tumor sections. RESULTS: Ascites pO2 was 0.90 ± 0.53 mm Hg. Single cancer cells and clusters suspended in ascites fluid as well as submillimeter serosal tumors stained positive for pimonidazole and GLUT-1 and had high 18F-FDG uptake. In contrast, 18F-FDG uptake was significantly lower in normoxic portion (little pimonidazole binding or GLUT-1 expression) of larger serosal tumors or subcutaneous xenografts, which was not statistically different from that in the liver. CONCLUSIONS: Glucose demand (18F-FDG uptake) in severely hypoxic ascites carcinomas and hypoxic portion of larger tumors is significantly higher than in normoxic cancer cells. Warburg effect originally obtained from Ehrlich ascites carcinoma may not apply to normoxic cancer cells. Our findings may benefit the better understanding of 18F-FDG PET in oncology application.

Original languageEnglish (US)
Pages (from-to)240-247
Number of pages8
JournalTranslational Oncology
Volume7
Issue number2
DOIs
StatePublished - 2014
Externally publishedYes

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Fluorodeoxyglucose F18
Ascites
Facilitative Glucose Transport Proteins
Neoplasms
Carcinoma
Heterografts
Breast Neoplasms
Tumor Hypoxia
Partial Pressure
Frozen Sections
Autoradiography
Nude Mice
Non-Small Cell Lung Carcinoma
Colorectal Neoplasms
Oxygen
Glucose
Liver

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

18F-fluorodeoxyglucose uptake and tumor hypoxia : Revisit 18F-fluorodeoxyglucose in oncology application. / Li, Xiao Feng; Du, Yang; Ma, Yuanyuan; Postel, Gregory C.; Civelek, Ali.

In: Translational Oncology, Vol. 7, No. 2, 2014, p. 240-247.

Research output: Contribution to journalArticle

Li, Xiao Feng ; Du, Yang ; Ma, Yuanyuan ; Postel, Gregory C. ; Civelek, Ali. / 18F-fluorodeoxyglucose uptake and tumor hypoxia : Revisit 18F-fluorodeoxyglucose in oncology application. In: Translational Oncology. 2014 ; Vol. 7, No. 2. pp. 240-247.
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abstract = "This study revisited 18F-fluorodeoxyglucose (18F-FDG) uptake and its relationship to hypoxia in various tumor models. METHODS: We generated peritoneal carcinomatosis and subcutaneous xenografts of colorectal cancer HT29, breast cancer MDA-MB-231, and non-small cell lung cancer A549 cell lines in nude mice. The partial oxygen pressure (pO2) of ascites fluid was measured. 18F-FDG accumulation detected by digital autoradiography was related to tumor hypoxia visualized by pimonidazole binding and glucose transporter-1 (GLUT-1) in frozen tumor sections. RESULTS: Ascites pO2 was 0.90 ± 0.53 mm Hg. Single cancer cells and clusters suspended in ascites fluid as well as submillimeter serosal tumors stained positive for pimonidazole and GLUT-1 and had high 18F-FDG uptake. In contrast, 18F-FDG uptake was significantly lower in normoxic portion (little pimonidazole binding or GLUT-1 expression) of larger serosal tumors or subcutaneous xenografts, which was not statistically different from that in the liver. CONCLUSIONS: Glucose demand (18F-FDG uptake) in severely hypoxic ascites carcinomas and hypoxic portion of larger tumors is significantly higher than in normoxic cancer cells. Warburg effect originally obtained from Ehrlich ascites carcinoma may not apply to normoxic cancer cells. Our findings may benefit the better understanding of 18F-FDG PET in oncology application.",
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