TY - JOUR
T1 - 16S rRNA amplicon sequencing identifies microbiota associated with oral cancer, Human Papilloma Virus infection and surgical treatment
AU - Guerrero-Preston, Rafael
AU - Godoy-Vitorino, Filipa
AU - Jedlicka, Anne
AU - Rodríguez-Hilario, Arnold
AU - González, Herminio
AU - Bondy, Jessica
AU - Lawson, Fahcina
AU - Folawiyo, Oluwasina
AU - Michailidi, Christina
AU - Dziedzic, Amanda
AU - Thangavel, Rajagowthamee
AU - Hadar, Tal
AU - Noordhuis, Maartje G.
AU - Westra, William
AU - Koch, Wayne
AU - Sidransky, David
N1 - Funding Information:
This research was supported by National Cancer Institute grants U01CA84986, K01CA164092 and CA121113; National Institute of Dental and Craniofacial Research grants P50DE019032 Head and Neck Cancer SPORE, RC2 DE20957, and National Institute of General Medical Sciences grant P20 GM103475.
PY - 2016/8/1
Y1 - 2016/8/1
N2 - Systemic inflammatory events and localized disease, mediated by the microbiome, may be measured in saliva as head and neck squamous cell carcinoma (HNSCC) diagnostic and prognostic biomonitors. We used a 16S rRNA V3-V5 marker gene approach to compare the saliva microbiome in DNA isolated from Oropharyngeal (OPSCC), Oral Cavity Squamous Cell Carcinoma (OCSCC) patients and normal epithelium controls, to characterize the HNSCC saliva microbiota and examine their abundance before and after surgical resection. The analyses identified a predominance of Firmicutes, Proteobacteria and Bacteroidetes, with less frequent presence of Actinobacteria and Fusobacteria before surgery. At lower taxonomic levels, the most abundant genera were Streptococcus, Prevotella, Haemophilus, Lactobacillus and Veillonella, with lower numbers of Citrobacter and Neisseraceae genus Kingella. HNSCC patients had a significant loss in richness and diversity of microbiota species (p < 0.05) compared to the controls. Overall, the Operational Taxonomic Units network shows that the relative abundance of OTU's within genus Streptococcus, Dialister, and Veillonella can be used to discriminate tumor from control samples (p < 0.05). Tumor samples lost Neisseria, Aggregatibacter (Proteobacteria), Haemophillus (Firmicutes) and Leptotrichia (Fusobacteria). Paired taxa within family Enterobacteriaceae, together with genus Oribacterium, distinguish OCSCC samples from OPSCC and normal samples (p < 0.05). Similarly, only HPV positive samples have an abundance of genus Gemellaceae and Leuconostoc (p < 0.05). Longitudinal analyses of samples taken before and after surgery, revealed a reduction in the alpha diversity measure after surgery, together with an increase of this measure in patients that recurred (p < 0.05). These results suggest that microbiota may be used as HNSCC diagnostic and prognostic biomonitors.
AB - Systemic inflammatory events and localized disease, mediated by the microbiome, may be measured in saliva as head and neck squamous cell carcinoma (HNSCC) diagnostic and prognostic biomonitors. We used a 16S rRNA V3-V5 marker gene approach to compare the saliva microbiome in DNA isolated from Oropharyngeal (OPSCC), Oral Cavity Squamous Cell Carcinoma (OCSCC) patients and normal epithelium controls, to characterize the HNSCC saliva microbiota and examine their abundance before and after surgical resection. The analyses identified a predominance of Firmicutes, Proteobacteria and Bacteroidetes, with less frequent presence of Actinobacteria and Fusobacteria before surgery. At lower taxonomic levels, the most abundant genera were Streptococcus, Prevotella, Haemophilus, Lactobacillus and Veillonella, with lower numbers of Citrobacter and Neisseraceae genus Kingella. HNSCC patients had a significant loss in richness and diversity of microbiota species (p < 0.05) compared to the controls. Overall, the Operational Taxonomic Units network shows that the relative abundance of OTU's within genus Streptococcus, Dialister, and Veillonella can be used to discriminate tumor from control samples (p < 0.05). Tumor samples lost Neisseria, Aggregatibacter (Proteobacteria), Haemophillus (Firmicutes) and Leptotrichia (Fusobacteria). Paired taxa within family Enterobacteriaceae, together with genus Oribacterium, distinguish OCSCC samples from OPSCC and normal samples (p < 0.05). Similarly, only HPV positive samples have an abundance of genus Gemellaceae and Leuconostoc (p < 0.05). Longitudinal analyses of samples taken before and after surgery, revealed a reduction in the alpha diversity measure after surgery, together with an increase of this measure in patients that recurred (p < 0.05). These results suggest that microbiota may be used as HNSCC diagnostic and prognostic biomonitors.
KW - 16s rRNA
KW - Human papilloma virus (HPV)
KW - Microbiome
KW - Oral cancer
KW - Oropharyngeal cancer
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UR - http://www.scopus.com/inward/citedby.url?scp=84982298141&partnerID=8YFLogxK
U2 - 10.18632/oncotarget.9710
DO - 10.18632/oncotarget.9710
M3 - Article
C2 - 27259999
AN - SCOPUS:84982298141
SN - 1949-2553
VL - 7
SP - 51320
EP - 51334
JO - Oncotarget
JF - Oncotarget
IS - 32
ER -