14-3-3 proteins tune non-muscle myosin II assembly

Hoku West-Foyle, Priyanka Kothari, Jonathan Osborne, Douglas N. Robinson

Research output: Contribution to journalArticle

Abstract

The 14-3-3 family comprises a group of small proteins that are essential, ubiquitous, and highly conserved across eukaryotes. Overexpression of the 14-3-3 proteins , , , and correlates with high metastatic potential in multiple cancer types. In Dictyostelium, 14-3-3 promotes myosin II turnover in the cell cortex and modulates cortical tension, cell shape, and cytokinesis. In light of the important roles of 14-3-3 proteins across a broad range of eukaryotic species, we sought to determine how 14-3-3 proteins interact with myosin II. Here, conducting in vitro and in vivo studies of both Dictyostelium (one 14-3-3 and one myosin II) and human proteins (seven 14-3-3s and three nonmuscle myosin IIs), we investigated the mechanism by which 14-3-3 proteins regulate myosin II assembly. Using in vitro assembly assays with purified myosin II tail fragments and 14-3-3, we demonstrate that this interaction is direct and phosphorylation-independent. All seven human 14-3-3 proteins also altered assembly of at least one paralog of myosin II. Our findings indicate a mechanism of myosin II assembly regulation that is mechanistically conserved across a billion years of evolution from amebas to humans. We predict that altered 14-3-3 expression in humans inhibits the tumor suppressor myosin II, contributing to the changes in cell mechanics observed in many metastatic cancers.

LanguageEnglish (US)
Pages6751-6761
Number of pages11
JournalJournal of Biological Chemistry
Volume293
Issue number18
DOIs
StatePublished - Jan 1 2018

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Myosin Type II
14-3-3 Proteins
Smart cards
Proteins
Dictyostelium
Phosphorylation
Neoplasms
Amoeba
Cytokinesis
Cell Shape
Tumors
Assays
Mechanics
Eukaryota
Tail

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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14-3-3 proteins tune non-muscle myosin II assembly. / West-Foyle, Hoku; Kothari, Priyanka; Osborne, Jonathan; Robinson, Douglas N.

In: Journal of Biological Chemistry, Vol. 293, No. 18, 01.01.2018, p. 6751-6761.

Research output: Contribution to journalArticle

West-Foyle, H, Kothari, P, Osborne, J & Robinson, DN 2018, '14-3-3 proteins tune non-muscle myosin II assembly' Journal of Biological Chemistry, vol. 293, no. 18, pp. 6751-6761. DOI: 10.1074/jbc.M117.819391
West-Foyle H, Kothari P, Osborne J, Robinson DN. 14-3-3 proteins tune non-muscle myosin II assembly. Journal of Biological Chemistry. 2018 Jan 1;293(18):6751-6761. Available from, DOI: 10.1074/jbc.M117.819391
West-Foyle, Hoku ; Kothari, Priyanka ; Osborne, Jonathan ; Robinson, Douglas N./ 14-3-3 proteins tune non-muscle myosin II assembly. In: Journal of Biological Chemistry. 2018 ; Vol. 293, No. 18. pp. 6751-6761
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