14-3-3 protein in CSF reflects SIV-mediated pre-synaptic damage

Kristi L. Helke, Suzanne E. Queen, Joseph L Mankowski

Research output: Contribution to journalArticle

Abstract

HIV-associated neurocognitive disorders (HAND) remain prevalent despite effective combined anti-retroviral therapy (cART). Cognitive function has been shown to inversely correlate with decreased synaptic and dendritic density. In this study, macaques inoculated with SIV were examined over a 3-month course of infection to characterize the appearance of the neuronal damage marker 14-3-3 protein in CSF and to determine whether CSF 14-3-3 levels directly reflected synaptic alterations. SIV-infected macaques with 14-3-3 in CSF had significantly lower levels of the presynaptic protein synaptophysin in cortical grey matter. Synaptophysin levels were inversely correlated with amount of SIV RNA in the CNS. In contrast, levels of 14-3-3 in CSF did not correspond with either alterations in levels of the postsynaptic protein PSD-95 or viral replication in the brain. These findings suggest that the appearance of 14-3-3 in CSF during asymptomatic infection reflects pre-synaptic damage in SIV-infected macaques and thus may serve as a marker of the early synaptic alterations that underlie HIV-induced neurocognitive impairment.

Original languageEnglish (US)
Pages (from-to)281-287
Number of pages7
JournalCurrent HIV Research
Volume11
Issue number4
StatePublished - Jun 2013

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14-3-3 Proteins
Macaca
Synaptophysin
HIV
Asymptomatic Infections
Cognition
Proteins
RNA
Brain
Infection
Therapeutics

Keywords

  • 14-3-3
  • CSF
  • HIV
  • PSD-95
  • SIV
  • Synaptophysin

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Cite this

14-3-3 protein in CSF reflects SIV-mediated pre-synaptic damage. / Helke, Kristi L.; Queen, Suzanne E.; Mankowski, Joseph L.

In: Current HIV Research, Vol. 11, No. 4, 06.2013, p. 281-287.

Research output: Contribution to journalArticle

Helke, Kristi L. ; Queen, Suzanne E. ; Mankowski, Joseph L. / 14-3-3 protein in CSF reflects SIV-mediated pre-synaptic damage. In: Current HIV Research. 2013 ; Vol. 11, No. 4. pp. 281-287.
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