14-3-3 coordinates microtubules, rac, and myosin II to control cell mechanics and cytokinesis

Qiongqiong Zhou, Yee Seir Kee, Christopher C. Poirier, Christine Jelinek, Jonathan Osborne, Srikanth Divi, Alexandra Surcel, Marie E. Will, Ulrike S. Eggert, Annette Müller-Taubenberger, Pablo A Iglesias, Robert J. Cotter, Douglas Robinson

Research output: Contribution to journalArticle

Abstract

Background: During cytokinesis, regulatory signals are presumed to emanate from the mitotic spindle. However, what these signals are and how they lead to the spatiotemporal changes in the cortex structure, mechanics, and regional contractility are not well understood in any system. Results: To investigate pathways that link the microtubule network to the cortical changes that promote cytokinesis, we used chemical genetics in Dictyostelium to identify genetic suppressors of nocodazole, a microtubule depolymerizer. We identified 14-3-3 and found that it is enriched in the cortex, helps maintain steady-state microtubule length, contributes to normal cortical tension, modulates actin wave formation, and controls the symmetry and kinetics of cleavage furrow contractility during cytokinesis. Furthermore, 14-3-3 acts downstream of a Rac small GTPase (RacE), associates with myosin II heavy chain, and is needed to promote myosin II bipolar thick filament remodeling. Conclusions: 14-3-3 connects microtubules, Rac, and myosin II to control several aspects of cortical dynamics, mechanics, and cytokinesis cell shape change. Furthermore, 14-3-3 interacts directly with myosin II heavy chain to promote bipolar thick filament remodeling and distribution. Overall, 14-3-3 appears to integrate several critical cytoskeletal elements that drive two important processes - cytokinesis cell shape change and cell mechanics.

Original languageEnglish (US)
Pages (from-to)1881-1889
Number of pages9
JournalCurrent Biology
Volume20
Issue number21
DOIs
StatePublished - Nov 9 2010

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Myosin Type II
Cytokinesis
cytokinesis
myosin
Mechanics
mechanics
Microtubules
microtubules
Myosin Heavy Chains
Cell Shape
cells
Nocodazole
cortex
Monomeric GTP-Binding Proteins
Dictyostelium
mitotic spindle apparatus
Spindle Apparatus
guanosinetriphosphatase
Actins
furrows

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

14-3-3 coordinates microtubules, rac, and myosin II to control cell mechanics and cytokinesis. / Zhou, Qiongqiong; Kee, Yee Seir; Poirier, Christopher C.; Jelinek, Christine; Osborne, Jonathan; Divi, Srikanth; Surcel, Alexandra; Will, Marie E.; Eggert, Ulrike S.; Müller-Taubenberger, Annette; Iglesias, Pablo A; Cotter, Robert J.; Robinson, Douglas.

In: Current Biology, Vol. 20, No. 21, 09.11.2010, p. 1881-1889.

Research output: Contribution to journalArticle

Zhou, Q, Kee, YS, Poirier, CC, Jelinek, C, Osborne, J, Divi, S, Surcel, A, Will, ME, Eggert, US, Müller-Taubenberger, A, Iglesias, PA, Cotter, RJ & Robinson, D 2010, '14-3-3 coordinates microtubules, rac, and myosin II to control cell mechanics and cytokinesis', Current Biology, vol. 20, no. 21, pp. 1881-1889. https://doi.org/10.1016/j.cub.2010.09.048
Zhou, Qiongqiong ; Kee, Yee Seir ; Poirier, Christopher C. ; Jelinek, Christine ; Osborne, Jonathan ; Divi, Srikanth ; Surcel, Alexandra ; Will, Marie E. ; Eggert, Ulrike S. ; Müller-Taubenberger, Annette ; Iglesias, Pablo A ; Cotter, Robert J. ; Robinson, Douglas. / 14-3-3 coordinates microtubules, rac, and myosin II to control cell mechanics and cytokinesis. In: Current Biology. 2010 ; Vol. 20, No. 21. pp. 1881-1889.
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