TY - JOUR
T1 - 14-3-3 coordinates microtubules, rac, and myosin II to control cell mechanics and cytokinesis
AU - Zhou, Qiongqiong
AU - Kee, Yee Seir
AU - Poirier, Christopher C.
AU - Jelinek, Christine
AU - Osborne, Jonathan
AU - Divi, Srikanth
AU - Surcel, Alexandra
AU - Will, Marie E.
AU - Eggert, Ulrike S.
AU - Müller-Taubenberger, Annette
AU - Iglesias, Pablo A.
AU - Cotter, Robert J.
AU - Robinson, Douglas N.
N1 - Funding Information:
We thank the members of the Robinson laboratory for helpful comments on the manuscript and Cathy Kabacoff in particular for assistance with generating cell lines. This work was supported by an American Heart Fellowship (to Q.Z.), National Institutes of Health (NIH) grant GM066817 (to D.N.R.), American Cancer Society grant RSG CCG-114122 (to D.N.R.), National Science Foundation grant CCF 0621740 (to P.A.I. and D.N.R.), NIH grant GM86704 (to P.A.I. and D.N.R.), and NIH grant GM082834 (to U.S.E).
PY - 2010/11/9
Y1 - 2010/11/9
N2 - Background: During cytokinesis, regulatory signals are presumed to emanate from the mitotic spindle. However, what these signals are and how they lead to the spatiotemporal changes in the cortex structure, mechanics, and regional contractility are not well understood in any system. Results: To investigate pathways that link the microtubule network to the cortical changes that promote cytokinesis, we used chemical genetics in Dictyostelium to identify genetic suppressors of nocodazole, a microtubule depolymerizer. We identified 14-3-3 and found that it is enriched in the cortex, helps maintain steady-state microtubule length, contributes to normal cortical tension, modulates actin wave formation, and controls the symmetry and kinetics of cleavage furrow contractility during cytokinesis. Furthermore, 14-3-3 acts downstream of a Rac small GTPase (RacE), associates with myosin II heavy chain, and is needed to promote myosin II bipolar thick filament remodeling. Conclusions: 14-3-3 connects microtubules, Rac, and myosin II to control several aspects of cortical dynamics, mechanics, and cytokinesis cell shape change. Furthermore, 14-3-3 interacts directly with myosin II heavy chain to promote bipolar thick filament remodeling and distribution. Overall, 14-3-3 appears to integrate several critical cytoskeletal elements that drive two important processes - cytokinesis cell shape change and cell mechanics.
AB - Background: During cytokinesis, regulatory signals are presumed to emanate from the mitotic spindle. However, what these signals are and how they lead to the spatiotemporal changes in the cortex structure, mechanics, and regional contractility are not well understood in any system. Results: To investigate pathways that link the microtubule network to the cortical changes that promote cytokinesis, we used chemical genetics in Dictyostelium to identify genetic suppressors of nocodazole, a microtubule depolymerizer. We identified 14-3-3 and found that it is enriched in the cortex, helps maintain steady-state microtubule length, contributes to normal cortical tension, modulates actin wave formation, and controls the symmetry and kinetics of cleavage furrow contractility during cytokinesis. Furthermore, 14-3-3 acts downstream of a Rac small GTPase (RacE), associates with myosin II heavy chain, and is needed to promote myosin II bipolar thick filament remodeling. Conclusions: 14-3-3 connects microtubules, Rac, and myosin II to control several aspects of cortical dynamics, mechanics, and cytokinesis cell shape change. Furthermore, 14-3-3 interacts directly with myosin II heavy chain to promote bipolar thick filament remodeling and distribution. Overall, 14-3-3 appears to integrate several critical cytoskeletal elements that drive two important processes - cytokinesis cell shape change and cell mechanics.
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U2 - 10.1016/j.cub.2010.09.048
DO - 10.1016/j.cub.2010.09.048
M3 - Article
C2 - 20951045
AN - SCOPUS:78149361673
SN - 0960-9822
VL - 20
SP - 1881
EP - 1889
JO - Current Biology
JF - Current Biology
IS - 21
ER -