| Original language | English (US) |
|---|---|
| Pages (from-to) | 530-540 |
| Number of pages | 11 |
| Journal | Methods in enzymology |
| Volume | 12 |
| Issue number | PART B |
| DOIs | |
| State | Published - 1968 |
| Externally published | Yes |
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
Cite this
- APA
- Standard
- Harvard
- Vancouver
- Author
- BIBTEX
- RIS
In: Methods in enzymology, Vol. 12, No. PART B, 1968, p. 530-540.
Research output: Contribution to journal › Article › peer-review
}
TY - JOUR
T1 - [137] Phage Qβ RNA polymerase
AU - Eoyang, Lillian
AU - August, J. T.
N1 - Funding Information: The replication of RNA viruses requires an enzymatic mechanism for the synthesis of nucleic acid utilizing RNA as the template. It is assumed that this reaction occurs in a manner analogous to that of DNA-directed synthesis of RNA, as depicted above. An enzyme utilizing RNA as template and ribonucleoside triphosphates as substrates is not known to occur in uninfected animal, plant, or bacterial cells. Rather, this activity has been found only when cells are infected with RNA viruses, la-ll Partially purified enzymes have been obtained from several sources? TM The procedure described below yields a 2000-5000 fold purified Q# RNA polymerase with an activity of 4000-5000 millimicromoles GMP incorporated per milligram of protein per 20 minutes. Another procedure 1 This work was supported in part by grants from the National Institutes of Health (GM-I1301 and GM-11936), and the National Science Foundation (GB-5082). Dr. J. T. August is recipient of an Investigatorship of the Health Research Council of the City of New York under contract 1-346. This is Communication No. 73 from the Joan and Lester Avnet Institute of Molecular Biology. 1, D. Baltimore and R. M. Franklin, Biochem. Biophys. Res. Commun. 9, 388 (1962). 'I. Haruna, K. Nozu, Y. Ohtaka, and S. Spiegelman, Proc. Natl. Aead. Eci. U.S. 50, 905 (1963). s j. T. August, S. Cooper, L. Shapiro, and N. D. Zinder, Cold Bpring Harbor Syrup. Quant. Biol. 28, 95 (1963). ' C. Weissmann, L. Simon, and S. Ochoa, Proc. Natl. Acad. Sci. U.8. 49, 407 (1963). M. J. Cline, R. Eason, and R. M. S. Smellie, J. Biol. Chem. 238, 1788 (1963). * S. Astier-Manifacier and P. Comuet, Biochem. Biophys. Res. Commun. 18, 283 (1965). R. G. Wilson and J. P. Bader, Biochim. Biophys. Acta 103, 549 (1965). 8 p. P. K. Ho and C. P. Walters, Biochemistry 5, 231 (1966). 9p. G. W. Plagemann and H. E. Swim, J. Bacteriol. 91, 2327 (1966). I°E. Horton, S. L. Lui, E. M. Martin, and T. S. Work, J. Mol. Biol. 15, 62 (1966). ~1G. Lust, J. Bacterlol. 91, 1612 (1966). C. Weiasmann, P. Borst, R. H. Burdon, M. A. Billeter, and S. Ochoa, Proc. Natl. Acad. Scl. U.S. 51, 890 (1964). 13j. T. August, L. Shapiro, and L. Eoyang, J. Mol. Biol. 11, 257 (1965). ~4I. Haruna and S. Spiegelman, Proc. Na~l. Acad. 8ei. U.8. 54, 579 (1965). Funding Information: This investigation was supported by Public Health Service Research Grant No. CA-01094 from the National Cancer Institute and Grant No. GB-4876 from the National Science Foundation. Postdoctoral trainee in Microbial and Molecular Genetics. Grant No. USPH-5-TO1-GM-319. *Present address: Dr. Ichiro Haruna, Department of Molecular Biology, School of Medicine, Keio University, Shinjuku-ku, Tokyo, Japan.
PY - 1968
Y1 - 1968
UR - http://www.scopus.com/inward/record.url?scp=0343842225&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0343842225&partnerID=8YFLogxK
U2 - 10.1016/0076-6879(67)12164-2
DO - 10.1016/0076-6879(67)12164-2
M3 - Article
AN - SCOPUS:0343842225
SN - 0076-6879
VL - 12
SP - 530
EP - 540
JO - Methods in enzymology
JF - Methods in enzymology
IS - PART B
ER -