1,25-Dihydroxyvitamin D3 transcriptionally activates the β3-integrin subunit gene in avian osteoclast precursors

Hirohide Mimura, Xu Cao, F. Patrick Ross, Mirei Chiba, Steven L. Teitelbaum

Research output: Contribution to journalArticle

Abstract

Osteoclasts are polykaryons and the principal, if not exclusive, resorptive cell of bone. They are members of the monocyte/macrophage family whose precursors differentiate under the influence of 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3]. Bone resorption is dependent on osteoclast-bone attachment, and we have shown that the integrin α(v)β3 is critical to the resorptive process. Thus, we asked whether 1,25-(OH)2D3 enhances the expression of α(v)β3 on the surface of osteoclast precursors and if the steroid modulates expression of the β3-integrin subunit. We found that 1,25-(OH)2D3 promotes the plasma membrane appearance of α(v)β3 on avian bone marrow-derived osteoclast precursors and does so at physiological concentrations (10-11 M) of the steroid. The effect is time dependent, appearing within 1 day of treatment. A full-length avian cDNA was cloned to explore the molecular mechanisms of β3 expression. The deduced amino acid sequence of the cDNA is 81% identical and 89% similar to that of human β3. Northern analysis demonstrates that β3 mRNA levels in vitamin D-treated osteoclast precursors mirror protein expression. Nuclear run-on experiments document the transcriptional nature of the event.

Original languageEnglish (US)
Pages (from-to)1061-1066
Number of pages6
JournalEndocrinology
Volume134
Issue number3
DOIs
StatePublished - Mar 1994
Externally publishedYes

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Calcitriol
Osteoclasts
Integrins
Genes
Complementary DNA
Steroids
Bone and Bones
Protein Precursors
Bone Resorption
Vitamin D
Monocytes
Amino Acid Sequence
Bone Marrow
Macrophages
Cell Membrane
Messenger RNA

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

1,25-Dihydroxyvitamin D3 transcriptionally activates the β3-integrin subunit gene in avian osteoclast precursors. / Mimura, Hirohide; Cao, Xu; Ross, F. Patrick; Chiba, Mirei; Teitelbaum, Steven L.

In: Endocrinology, Vol. 134, No. 3, 03.1994, p. 1061-1066.

Research output: Contribution to journalArticle

Mimura, Hirohide ; Cao, Xu ; Ross, F. Patrick ; Chiba, Mirei ; Teitelbaum, Steven L. / 1,25-Dihydroxyvitamin D3 transcriptionally activates the β3-integrin subunit gene in avian osteoclast precursors. In: Endocrinology. 1994 ; Vol. 134, No. 3. pp. 1061-1066.
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