10-valent pneumococcal non-typeable Haemophilus influenzae protein-D conjugate vaccine (PHiD-CV) induces memory B cell responses in healthy Kenyan toddlers

D. M. Muema, E. W. Nduati, M. Uyoga, M. Bashraheil, J. A G Scott, Laura L Hammitt, B. C. Urban

Research output: Contribution to journalArticle

Abstract

Memory B cells are long-lived and could contribute to persistence of humoral immunity by maintaining the plasma-cell pool or making recall responses upon re-exposure to an antigen. We determined the ability of a pneumococcal conjugate vaccine to induce anti-pneumococcal memory B cells. Frequencies of memory B cells against pneumococcal capsular polysaccharides from serotypes 1, 6B, 14, 19F and 23F were determined by cultured B cell enzyme-linked immunospot (ELISPOT) in 35 children aged 12-23 months who received pneumococcal non-typeable Haemophilus influenzae protein-D conjugate vaccine (PHiD-CV). The relationships between plasma antibodies and memory B cell frequencies were also assessed. After two doses of PHiD-CV, the proportion of subjects with detectable memory B cells against pneumococcal capsular polysaccharides increased significantly for serotypes 1 (3-45%; P

Original languageEnglish (US)
Pages (from-to)297-305
Number of pages9
JournalClinical and Experimental Immunology
Volume181
Issue number2
DOIs
StatePublished - 2015

Fingerprint

Conjugate Vaccines
B-Lymphocytes
Polysaccharides
Pneumococcal Vaccines
Humoral Immunity
Plasma Cells
Haemophilus influenzae glpQ protein
Cultured Cells
Antigens
Antibodies
Enzymes

Keywords

  • Children
  • ELISPOT
  • Memory B cells
  • PHiD-CV
  • Pneumococcal conjugate vaccines

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

10-valent pneumococcal non-typeable Haemophilus influenzae protein-D conjugate vaccine (PHiD-CV) induces memory B cell responses in healthy Kenyan toddlers. / Muema, D. M.; Nduati, E. W.; Uyoga, M.; Bashraheil, M.; Scott, J. A G; Hammitt, Laura L; Urban, B. C.

In: Clinical and Experimental Immunology, Vol. 181, No. 2, 2015, p. 297-305.

Research output: Contribution to journalArticle

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