[10] 5-Fluoroorotic acid as a selective agent in yeast molecular genetics

Jef D. Boeke; Joshua Trueheart; Georges Natsoulis; Gerald R. Fink

doi:10.1016/0076-6879(87)54076-9

[10] 5-Fluoroorotic acid as a selective agent in yeast molecular genetics

Jef D. Boeke, Joshua Trueheart, Georges Natsoulis, Gerald R. Fink

Research output: Contribution to journal › Article › peer-review

1039 Scopus citations

Abstract

5-FOA is an extremely useful reagent for the selection of Ura^- cells amid a population of Ura⁺ cells. The selection is effective in transformation and recombination studies where loss of URA3⁺ is desired. A new plasmid shuffling procedure based on the 5-FOA^R selection permits the recovery of conditional lethal mutations in cloned genes that encode vital functions.

Original language	English (US)
Pages (from-to)	164-175
Number of pages	12
Journal	Methods in enzymology
Volume	154
Issue number	C
DOIs	https://doi.org/10.1016/0076-6879(87)54076-9
State	Published - 1987
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Molecular Biology

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10.1016/0076-6879(87)54076-9

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title = "[10] 5-Fluoroorotic acid as a selective agent in yeast molecular genetics",

abstract = "5-FOA is an extremely useful reagent for the selection of Ura- cells amid a population of Ura+ cells. The selection is effective in transformation and recombination studies where loss of URA3+ is desired. A new plasmid shuffling procedure based on the 5-FOAR selection permits the recovery of conditional lethal mutations in cloned genes that encode vital functions.",

author = "Boeke, {Jef D.} and Joshua Trueheart and Georges Natsoulis and Fink, {Gerald R.}",

note = "Funding Information: J.D.B. was supported by a Helen Hay Whitney Fellowship, G.N. is a fellow of the Belgian Fonds National de la Recherche Scientifique,G .R.F. is an AmericanC ancer Society Professor. Copyright: Copyright 2010 Elsevier B.V., All rights reserved.",

year = "1987",

doi = "10.1016/0076-6879(87)54076-9",

language = "English (US)",

volume = "154",

pages = "164--175",

journal = "Methods in enzymology",

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publisher = "Academic Press Inc.",

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T1 - [10] 5-Fluoroorotic acid as a selective agent in yeast molecular genetics

AU - Boeke, Jef D.

AU - Trueheart, Joshua

AU - Natsoulis, Georges

AU - Fink, Gerald R.

N1 - Funding Information: J.D.B. was supported by a Helen Hay Whitney Fellowship, G.N. is a fellow of the Belgian Fonds National de la Recherche Scientifique,G .R.F. is an AmericanC ancer Society Professor. Copyright: Copyright 2010 Elsevier B.V., All rights reserved.

PY - 1987

Y1 - 1987

N2 - 5-FOA is an extremely useful reagent for the selection of Ura- cells amid a population of Ura+ cells. The selection is effective in transformation and recombination studies where loss of URA3+ is desired. A new plasmid shuffling procedure based on the 5-FOAR selection permits the recovery of conditional lethal mutations in cloned genes that encode vital functions.

AB - 5-FOA is an extremely useful reagent for the selection of Ura- cells amid a population of Ura+ cells. The selection is effective in transformation and recombination studies where loss of URA3+ is desired. A new plasmid shuffling procedure based on the 5-FOAR selection permits the recovery of conditional lethal mutations in cloned genes that encode vital functions.

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JO - Methods in enzymology

JF - Methods in enzymology

IS - C

ER -

[10] 5-Fluoroorotic acid as a selective agent in yeast molecular genetics

Abstract

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