[10] 5-Fluoroorotic acid as a selective agent in yeast molecular genetics

Jef D. Boeke, Joshua Trueheart, Georges Natsoulis, Gerald R. Fink

Research output: Contribution to journalArticle

Abstract

5-FOA is an extremely useful reagent for the selection of Ura- cells amid a population of Ura+ cells. The selection is effective in transformation and recombination studies where loss of URA3+ is desired. A new plasmid shuffling procedure based on the 5-FOAR selection permits the recovery of conditional lethal mutations in cloned genes that encode vital functions.

Original languageEnglish (US)
Pages (from-to)164-175
Number of pages12
JournalMethods in Enzymology
Volume154
Issue numberC
DOIs
StatePublished - 1987
Externally publishedYes

Fingerprint

Yeast
Molecular Biology
Plasmids
Genes
Yeasts
Recovery
Genetic Recombination
Mutation
Population
Genetics
5-fluoroorotic acid

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

[10] 5-Fluoroorotic acid as a selective agent in yeast molecular genetics. / Boeke, Jef D.; Trueheart, Joshua; Natsoulis, Georges; Fink, Gerald R.

In: Methods in Enzymology, Vol. 154, No. C, 1987, p. 164-175.

Research output: Contribution to journalArticle

Boeke, Jef D. ; Trueheart, Joshua ; Natsoulis, Georges ; Fink, Gerald R. / [10] 5-Fluoroorotic acid as a selective agent in yeast molecular genetics. In: Methods in Enzymology. 1987 ; Vol. 154, No. C. pp. 164-175.
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