[10] 5-Fluoroorotic acid as a selective agent in yeast molecular genetics

Jef D. Boeke, Joshua Trueheart, Georges Natsoulis, Gerald R. Fink

Research output: Contribution to journalArticle

Abstract

5-FOA is an extremely useful reagent for the selection of Ura- cells amid a population of Ura+ cells. The selection is effective in transformation and recombination studies where loss of URA3+ is desired. A new plasmid shuffling procedure based on the 5-FOAR selection permits the recovery of conditional lethal mutations in cloned genes that encode vital functions.

Original languageEnglish (US)
Pages (from-to)164-175
Number of pages12
JournalMethods in enzymology
Volume154
Issue numberC
DOIs
StatePublished - Dec 1 1987

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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