1-DE MS and 2-D LC-MS analysis of the mouse bronchoalveolar lavage proteome

Yurong Guo; Shwu Fan Ma; Dmitry Grigoryev; Jennifer Van Eyk; Joe G.N. Garcia

doi:10.1002/pmic.200500052

1-DE MS and 2-D LC-MS analysis of the mouse bronchoalveolar lavage proteome

Yurong Guo, Shwu Fan Ma, Dmitry Grigoryev, Jennifer Van Eyk, Joe G.N. Garcia

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

Bronchoalveolar lavage fluid (BALF) is a complex mixture of proteins, which represents a unique clinically useful sampling of the lower respiratory tract. Many proteomic technologies can be used to characterize complex biological mixtures; however, it is not yet clear which technology(s) provide more information regarding the number of proteins identified and sequence coverage. In this study, we initially compared two common proteomic approaches, 2-D LC microESI MS/ MS and 1-DE followed by gel slice digestion, peptide extraction and peptide identification by MS in characterization of the mouse BALF proteome; secondly, we identified 297 unique proteins from the mouse BALF proteome, greatly expanded the BALF proteome by about threefold regardless of species.

Original language	English (US)
Pages (from-to)	4608-4624
Number of pages	17
Journal	Proteomics
Volume	5
Issue number	17
DOIs	https://doi.org/10.1002/pmic.200500052
State	Published - Nov 2005
Externally published	Yes

Keywords

1-DE
Bronchoalveolar lavage
LC-MS/MS
Mouse
Proteome analysis

ASJC Scopus subject areas

Biochemistry
Molecular Biology

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10.1002/pmic.200500052

Cite this

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title = "1-DE MS and 2-D LC-MS analysis of the mouse bronchoalveolar lavage proteome",

abstract = "Bronchoalveolar lavage fluid (BALF) is a complex mixture of proteins, which represents a unique clinically useful sampling of the lower respiratory tract. Many proteomic technologies can be used to characterize complex biological mixtures; however, it is not yet clear which technology(s) provide more information regarding the number of proteins identified and sequence coverage. In this study, we initially compared two common proteomic approaches, 2-D LC microESI MS/ MS and 1-DE followed by gel slice digestion, peptide extraction and peptide identification by MS in characterization of the mouse BALF proteome; secondly, we identified 297 unique proteins from the mouse BALF proteome, greatly expanded the BALF proteome by about threefold regardless of species.",

keywords = "1-DE, Bronchoalveolar lavage, LC-MS/MS, Mouse, Proteome analysis",

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AU - Guo, Yurong

AU - Ma, Shwu Fan

AU - Grigoryev, Dmitry

AU - Van Eyk, Jennifer

AU - Garcia, Joe G.N.

PY - 2005/11

Y1 - 2005/11

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AB - Bronchoalveolar lavage fluid (BALF) is a complex mixture of proteins, which represents a unique clinically useful sampling of the lower respiratory tract. Many proteomic technologies can be used to characterize complex biological mixtures; however, it is not yet clear which technology(s) provide more information regarding the number of proteins identified and sequence coverage. In this study, we initially compared two common proteomic approaches, 2-D LC microESI MS/ MS and 1-DE followed by gel slice digestion, peptide extraction and peptide identification by MS in characterization of the mouse BALF proteome; secondly, we identified 297 unique proteins from the mouse BALF proteome, greatly expanded the BALF proteome by about threefold regardless of species.

KW - 1-DE

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KW - LC-MS/MS

KW - Mouse

KW - Proteome analysis

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1-DE MS and 2-D LC-MS analysis of the mouse bronchoalveolar lavage proteome

Abstract

Keywords

ASJC Scopus subject areas

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