σ2 Receptors regulate changes in sphingolipid levels in breast tumor cells

Keith W. Crawford, Andrew Coop, Wayne D. Bowen

Research output: Contribution to journalArticlepeer-review

Abstract

σ2 Receptors induce apoptosis in various cell types. The sphingolipid, ceramide as well as the sphingoid bases are involved in cell proliferation. Sphingolipids of MCF-7/Adr- and T47D breast tumor cells were metabolically radiolabeled. The σ2 receptor agonists (+)-1R,5R-E-8-(3,4-dichlorobenzylidene)-5-(3-hydroxyphenyl)-2-methylmorphan- 7-one (CB-184) and 1S,2R-(-)-cis-N-[2-(3,4-dichlorophenyl)ethyl]-N-methyl-2-(1-pyrrolidinyl)- cyclohexylamine (BD737) caused dose-dependent increases in [3H]ceramide, with concomitant decreases in [3H]sphingomyelin. Both effects were attenuated by the novel σ2 receptor antagonist, N-phenethylpiperidine oxalate (AC927). σ2 Receptors may produce effects on cell growth and apoptosis by regulating the sphingolipid pathway.

Original languageEnglish (US)
Pages (from-to)207-209
Number of pages3
JournalEuropean Journal of Pharmacology
Volume443
Issue number1-3
DOIs
StatePublished - May 17 2002

Keywords

  • Apoptosis
  • Ceramide
  • σ Receptor

ASJC Scopus subject areas

  • Pharmacology

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