μl Na+ channels expressed transiently in human embryonic kidney cells: Biochemical and biophysical properties

Chinweike Ukomadu, Jiuying Zhou, Fred J. Sigworth, William S. Agnew

Research output: Contribution to journalArticle

Abstract

We describe the transient expression of the rat skeletal muscle μl Na+ channel in human embryonic kidney (HEK 293) cells. Functional channels appear at a density of ∼30 in a 10 μm2 patch, comparable to those of native excitable cells. Unlike pl currents in oocytes, inactivation gating is predominantly (∼97%) fast, although clear evidence is provided for noninactivating gating modes, which have been linked to anomalous behavior in the inherited disorder hyperkalemic periodic paralysis. Sequence-specific antibodies detect a ∼230 kd glycopeptide. The majority of molecules acquire only neutral oligosaccharides and are retained within the cell. Electrophoretic mobility on SDS gels suggests the molecules may acquire covalently attached lipid. The channel is readily phosphorylated by activation of the protein kinase A and protein kinase C second messenger pathways.

Original languageEnglish (US)
Pages (from-to)663-676
Number of pages14
JournalNeuron
Volume8
Issue number4
DOIs
StatePublished - Apr 1992
Externally publishedYes

ASJC Scopus subject areas

  • Neuroscience(all)

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