μ2 adaptin facilitates but is not essential for synaptic vesicle recycling in Caenorhabditis elegans

Mingyu Gu, Kim Schuske, Shigeki Watanabe, Qiang Liu, Paul Baum, Gian Garriga, Erik M. Jorgensen

Research output: Contribution to journalArticlepeer-review

Abstract

Synaptic vesicles must be recycled to sustain neuro-transmission, in large part via clathrin-mediated endocytosis. Clathrin is recruited to endocytic sites on the plasma membrane by the AP2 adaptor complex. The medium subunit (μ2) of AP2 binds to cargo proteins and phosphatidylinositol-4,5-bisphosphate on the cell surface. Here, we characterize the apm-2 gene (also called dpy-23), which encodes the only μ2 subunit in the nematode Caenorhabditis elegans. APM-2 is highly expressed in the nervous system and is localized to synapses; yet specific loss of APM-2 in neurons does not affect locomotion. In apm-2 mutants, clathrin is mislocalized at synapses, and synaptic vesicle numbers and evoked responses are reduced to 60 and 65%, respectively. Collectively, these data suggest AP2 μ2 facilitates but is not essential for synaptic vesicle recycling.

Original languageEnglish (US)
Pages (from-to)881-892
Number of pages12
JournalJournal of Cell Biology
Volume183
Issue number5
DOIs
StatePublished - Dec 1 2008
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology

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