TY - JOUR
T1 - μ2 adaptin facilitates but is not essential for synaptic vesicle recycling in Caenorhabditis elegans
AU - Gu, Mingyu
AU - Schuske, Kim
AU - Watanabe, Shigeki
AU - Liu, Qiang
AU - Baum, Paul
AU - Garriga, Gian
AU - Jorgensen, Erik M.
PY - 2008/12/1
Y1 - 2008/12/1
N2 - Synaptic vesicles must be recycled to sustain neuro-transmission, in large part via clathrin-mediated endocytosis. Clathrin is recruited to endocytic sites on the plasma membrane by the AP2 adaptor complex. The medium subunit (μ2) of AP2 binds to cargo proteins and phosphatidylinositol-4,5-bisphosphate on the cell surface. Here, we characterize the apm-2 gene (also called dpy-23), which encodes the only μ2 subunit in the nematode Caenorhabditis elegans. APM-2 is highly expressed in the nervous system and is localized to synapses; yet specific loss of APM-2 in neurons does not affect locomotion. In apm-2 mutants, clathrin is mislocalized at synapses, and synaptic vesicle numbers and evoked responses are reduced to 60 and 65%, respectively. Collectively, these data suggest AP2 μ2 facilitates but is not essential for synaptic vesicle recycling.
AB - Synaptic vesicles must be recycled to sustain neuro-transmission, in large part via clathrin-mediated endocytosis. Clathrin is recruited to endocytic sites on the plasma membrane by the AP2 adaptor complex. The medium subunit (μ2) of AP2 binds to cargo proteins and phosphatidylinositol-4,5-bisphosphate on the cell surface. Here, we characterize the apm-2 gene (also called dpy-23), which encodes the only μ2 subunit in the nematode Caenorhabditis elegans. APM-2 is highly expressed in the nervous system and is localized to synapses; yet specific loss of APM-2 in neurons does not affect locomotion. In apm-2 mutants, clathrin is mislocalized at synapses, and synaptic vesicle numbers and evoked responses are reduced to 60 and 65%, respectively. Collectively, these data suggest AP2 μ2 facilitates but is not essential for synaptic vesicle recycling.
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U2 - 10.1083/jcb.200806088
DO - 10.1083/jcb.200806088
M3 - Article
C2 - 19047463
AN - SCOPUS:58149279416
VL - 183
SP - 881
EP - 892
JO - Journal of Cell Biology
JF - Journal of Cell Biology
SN - 0021-9525
IS - 5
ER -