Abstract
Vascular remodeling is a complex process critical to development of the mature vascular system. Astrocytes are known to be indispensable for initial formation of the retinal vasculature; our studies with the Nuc1 rat provide novel evidence that these cells are also essential in the retinal vascular remodeling process. Nuc1 is a spontaneous mutation in the Sprague-Dawley rat originally characterized by nuclear cataracts in the heterozygote and microphthalmia in the homozygote. We report here that the Nuc1 allele results from mutation of the βA3/A1-crystallin gene, which in the neural retina is expressed only in astrocytes. We demonstrate striking structural abnormalities in Nuc1 astrocytes with profound effects on the organization of intermediate filaments. While vessels form in the Nuc1 retina, the subsequent remodeling process required to provide a mature vascular network is deficient. Our data implicate βA3/A1-crystallin as an important regulatory factor mediating vascular patterning and remodeling in the retina.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 85-95 |
| Number of pages | 11 |
| Journal | Molecular and Cellular Neuroscience |
| Volume | 37 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 2008 |
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Keywords
- βA3/A1-crystallin
- Astrocytes
- Intermediate filaments
- Rat spontaneous mutation
- Remodeling
- Retinal vasculature
ASJC Scopus subject areas
- Molecular Biology
- Cellular and Molecular Neuroscience
- Developmental Neuroscience
Cite this
βA3/A1-crystallin in astroglial cells regulates retinal vascular remodeling during development. / Sinha, Debasish; Klise, Andrew; Sergeev, Yuri; Hose, Stacey; Bhutto, Imran A.; Hackler, Laszlo; Malpic-llanos, Tanya; Samtani, Sonia; Grebe, Rhonda; Goldberg, Morton F; Hejtmancik, J. Fielding; Nath, Avindra; Zack, Donald J; Fariss, Robert N.; McLeod, D. Scott; Sundin, Olof; Broman, Karl W.; Lutty, Gerard Anthony; Zigler, J. Samuel.
In: Molecular and Cellular Neuroscience, Vol. 37, No. 1, 01.2008, p. 85-95.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - βA3/A1-crystallin in astroglial cells regulates retinal vascular remodeling during development
AU - Sinha, Debasish
AU - Klise, Andrew
AU - Sergeev, Yuri
AU - Hose, Stacey
AU - Bhutto, Imran A.
AU - Hackler, Laszlo
AU - Malpic-llanos, Tanya
AU - Samtani, Sonia
AU - Grebe, Rhonda
AU - Goldberg, Morton F
AU - Hejtmancik, J. Fielding
AU - Nath, Avindra
AU - Zack, Donald J
AU - Fariss, Robert N.
AU - McLeod, D. Scott
AU - Sundin, Olof
AU - Broman, Karl W.
AU - Lutty, Gerard Anthony
AU - Zigler, J. Samuel
PY - 2008/1
Y1 - 2008/1
N2 - Vascular remodeling is a complex process critical to development of the mature vascular system. Astrocytes are known to be indispensable for initial formation of the retinal vasculature; our studies with the Nuc1 rat provide novel evidence that these cells are also essential in the retinal vascular remodeling process. Nuc1 is a spontaneous mutation in the Sprague-Dawley rat originally characterized by nuclear cataracts in the heterozygote and microphthalmia in the homozygote. We report here that the Nuc1 allele results from mutation of the βA3/A1-crystallin gene, which in the neural retina is expressed only in astrocytes. We demonstrate striking structural abnormalities in Nuc1 astrocytes with profound effects on the organization of intermediate filaments. While vessels form in the Nuc1 retina, the subsequent remodeling process required to provide a mature vascular network is deficient. Our data implicate βA3/A1-crystallin as an important regulatory factor mediating vascular patterning and remodeling in the retina.
AB - Vascular remodeling is a complex process critical to development of the mature vascular system. Astrocytes are known to be indispensable for initial formation of the retinal vasculature; our studies with the Nuc1 rat provide novel evidence that these cells are also essential in the retinal vascular remodeling process. Nuc1 is a spontaneous mutation in the Sprague-Dawley rat originally characterized by nuclear cataracts in the heterozygote and microphthalmia in the homozygote. We report here that the Nuc1 allele results from mutation of the βA3/A1-crystallin gene, which in the neural retina is expressed only in astrocytes. We demonstrate striking structural abnormalities in Nuc1 astrocytes with profound effects on the organization of intermediate filaments. While vessels form in the Nuc1 retina, the subsequent remodeling process required to provide a mature vascular network is deficient. Our data implicate βA3/A1-crystallin as an important regulatory factor mediating vascular patterning and remodeling in the retina.
KW - βA3/A1-crystallin
KW - Astrocytes
KW - Intermediate filaments
KW - Rat spontaneous mutation
KW - Remodeling
KW - Retinal vasculature
UR - http://www.scopus.com/inward/record.url?scp=37449027288&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=37449027288&partnerID=8YFLogxK
U2 - 10.1016/j.mcn.2007.08.016
DO - 10.1016/j.mcn.2007.08.016
M3 - Article
C2 - 17931883
AN - SCOPUS:37449027288
VL - 37
SP - 85
EP - 95
JO - Molecular and Cellular Neurosciences
JF - Molecular and Cellular Neurosciences
SN - 1044-7431
IS - 1
ER -