β2-adrenergic receptor supports prolonged theta tetanus-induced LTP

Hai Qian, Lucas Matt, Mingxu Zhang, Minh Nguyen, Tommaso Patriarchi, Olha M. Koval, Mark Anderson, Kaiwen He, Hey-Kyoung Lee, Johannes W. Hell

Research output: Contribution to journalArticle

Abstract

The Widespread noradrenergic innervations in the brain promotes arousal and learning by molecular mechanisms that remain largely undefined. Recent work shows that the β2-adrenergic receptor (β2AR) is linked to the AMPA-type glutamate receptor subunit GluA1 via stargazin and PSD-95 (Joiner ML, Lise MF, Yuen EY, Kam AY, Zhang M, Hall DD, Malik ZA, Qian H, Chen Y, Ulrich JD, Burette AC, Weinberg RJ, Law PY, El-Husseini A, Yan Z, Hell JW. EMBO J 29: 482-495, 2010). We now demonstrate that the β2AR plays a prominent role in long-term potentiation (LTP) induced by a train of 900 stimuli at 5 Hz (prolonged theta-tetanus-LTP, or PTT-LTP) in the hippocampal CA1 region in mice, which requires simultaneous β -adrenergic stimulation. Although PTT-LTP was impaired in hippocampal slices from β1AR and β 2AR knockout (KO) mice, only β 2AR-selective stimulation with salbutamol supported this PTT-LTP in wild-type (WT) slices, whereas β1AR-selective stimulation with dobutamine (+ prazosin) did not. Furthermore, only the β 2AR-selective antagonist ICI-118551 and not the β1AR-selective antagonist CGP-20712 inhibited PTT-LTP and phosphorylation of GluA1 on its PKA site S845 in WT slices. Our analysis of S845A knockin (KI) mice indicates that this phosphorylation is relevant for PTT-LTP. These results identify the β 2AR-S845 signaling pathway as a prominent regulator of synaptic plasticity.

Original languageEnglish (US)
Pages (from-to)2703-2712
Number of pages10
JournalJournal of Neurophysiology
Volume107
Issue number10
DOIs
StatePublished - May 15 2012
Externally publishedYes

Fingerprint

Long-Term Potentiation
Tetanus
Adrenergic Receptors
Phosphorylation
Hippocampal CA1 Region
Neuronal Plasticity
Adrenergic Antagonists
Dobutamine
AMPA Receptors
Albuterol
Prazosin
Glutamate Receptors
Arousal
Knockout Mice
Adrenergic Agents
Learning
Brain

Keywords

  • α3-hy-droxy-5-methylisoxazole propionic acid receptors
  • Beta-adrenergic signaling
  • Postsynaptic signaling
  • Protein kinase A

ASJC Scopus subject areas

  • Physiology
  • Neuroscience(all)

Cite this

Qian, H., Matt, L., Zhang, M., Nguyen, M., Patriarchi, T., Koval, O. M., ... Hell, J. W. (2012). β2-adrenergic receptor supports prolonged theta tetanus-induced LTP. Journal of Neurophysiology, 107(10), 2703-2712. https://doi.org/10.1152/jn.00374.2011

β2-adrenergic receptor supports prolonged theta tetanus-induced LTP. / Qian, Hai; Matt, Lucas; Zhang, Mingxu; Nguyen, Minh; Patriarchi, Tommaso; Koval, Olha M.; Anderson, Mark; He, Kaiwen; Lee, Hey-Kyoung; Hell, Johannes W.

In: Journal of Neurophysiology, Vol. 107, No. 10, 15.05.2012, p. 2703-2712.

Research output: Contribution to journalArticle

Qian, H, Matt, L, Zhang, M, Nguyen, M, Patriarchi, T, Koval, OM, Anderson, M, He, K, Lee, H-K & Hell, JW 2012, 'β2-adrenergic receptor supports prolonged theta tetanus-induced LTP', Journal of Neurophysiology, vol. 107, no. 10, pp. 2703-2712. https://doi.org/10.1152/jn.00374.2011
Qian H, Matt L, Zhang M, Nguyen M, Patriarchi T, Koval OM et al. β2-adrenergic receptor supports prolonged theta tetanus-induced LTP. Journal of Neurophysiology. 2012 May 15;107(10):2703-2712. https://doi.org/10.1152/jn.00374.2011
Qian, Hai ; Matt, Lucas ; Zhang, Mingxu ; Nguyen, Minh ; Patriarchi, Tommaso ; Koval, Olha M. ; Anderson, Mark ; He, Kaiwen ; Lee, Hey-Kyoung ; Hell, Johannes W. / β2-adrenergic receptor supports prolonged theta tetanus-induced LTP. In: Journal of Neurophysiology. 2012 ; Vol. 107, No. 10. pp. 2703-2712.
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