β1 integrins regulate myoblast fusion and sarcomere assembly

Martin Schwander, Marco Leu, Michael Stumm, Olivier M. Dorchies, Urs T. Ruegg, Johannes Schittny, Ulrich Müller

Research output: Contribution to journalArticlepeer-review

Abstract

The mechanisms that regulate the formation of multinucleated muscle fibers from mononucleated myoblasts are not well understood. We show here that extracellular matrix (ECM) receptors of the β1 integrin family regulate myoblast fusion. β1-deficient myoblasts adhere to each other, but plasma membrane breakdown is defective. The integrin-associated tetraspanin CD9 that regulates cell fusion is no longer expressed at the cell surface of β1-deficient myoblasts, suggesting that β1 integrins regulate the formation of a protein complex important for fusion. Subsequent to fusion, β1 integrins are required for the assembly of sarcomeres. Other ECM receptors such as the dystrophin glycoprotein complex are still expressed but cannot compensate for the loss of β1 integrins, providing evidence that different ECM receptors have nonredundant functions in skeletal muscle fibers.

Original languageEnglish (US)
Pages (from-to)673-685
Number of pages13
JournalDevelopmental Cell
Volume4
Issue number5
DOIs
StatePublished - May 1 2003
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Developmental Biology
  • Cell Biology

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