β-III spectrin is critical for development of Purkinje cell dendritic tree and spine morphogenesis

Yuanzheng Gao, Emma M. Perkins, Yvonne L. Clarkson, Steven Tobia, Alastair R. Lyndon, Mandy Jackson, Jeffrey D Rothstein

Research output: Contribution to journalArticle

Abstract

Mutations in the gene encoding β-III spectrin give rise to spinocerebellar ataxia type 5, a neurodegenerative disease characterized by progressive thinning of the molecular layer, loss of Purkinje cells and increasing motor deficits.Amouse lacking full-length β-III spectrin (β-III -/-) displays a similar phenotype. In vitro and in vivo analyses of Purkinje cells lacking β-III spectrin, reveal a critical role for β-III spectrin in Purkinje cell morphological development. Disruption of the normally well ordered dendritic arborization occurs in Purkinje cells from β-III -/- mice, specifically showing a loss of monoplanar organization, smaller average dendritic diameter and reduced densities of Purkinje cell spines and synapses. Early morphological defects appear to affect distribution of dendritic, but not axonal, proteins. This study confirms that thinning of the molecular layer associated with disease pathogenesis is a consequence of Purkinje cell dendritic degeneration, as Purkinje cells from 8-month-old β-III -/-mice have drastically reduced dendritic volumes, surface areas and total dendritic lengths compared with 5-to 6-week-old β-III -/-mice. These findings highlight a critical role of β-III spectrin in dendritic biology and are consistent with an early developmental defect in β-III -/- mice, with abnormal Purkinje cell dendritic morphology potentially underlying disease pathogenesis.

Original languageEnglish (US)
Pages (from-to)16581-16590
Number of pages10
JournalJournal of Neuroscience
Volume31
Issue number46
DOIs
StatePublished - Nov 16 2011

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Spectrin
Dendritic Spines
Purkinje Cells
Morphogenesis
Spinocerebellar Ataxias
Neuronal Plasticity
Neurodegenerative Diseases
Synapses
Spine
Phenotype
Mutation

ASJC Scopus subject areas

  • Neuroscience(all)

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β-III spectrin is critical for development of Purkinje cell dendritic tree and spine morphogenesis. / Gao, Yuanzheng; Perkins, Emma M.; Clarkson, Yvonne L.; Tobia, Steven; Lyndon, Alastair R.; Jackson, Mandy; Rothstein, Jeffrey D.

In: Journal of Neuroscience, Vol. 31, No. 46, 16.11.2011, p. 16581-16590.

Research output: Contribution to journalArticle

Gao, Yuanzheng ; Perkins, Emma M. ; Clarkson, Yvonne L. ; Tobia, Steven ; Lyndon, Alastair R. ; Jackson, Mandy ; Rothstein, Jeffrey D. / β-III spectrin is critical for development of Purkinje cell dendritic tree and spine morphogenesis. In: Journal of Neuroscience. 2011 ; Vol. 31, No. 46. pp. 16581-16590.
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