The details of a colorimetric method for the measurement of both β-d-galactosidase and N-acetyl-β-d-glucosaminidase activities in human urine are described. A study of a large number of both "normal and mentally retarded" individuals indicates that these 2 enzymes are present in high, but variable, concentrations in fresh urine samples from both groups. In contrast, the urine of a patient with generalized gangliosidosis was found repeatedly to have very low levels of the β-d-galactosidase while having very high levels of the N-acetyl-β-d-glucosaminidase. By relating the 2 enzyme levels as a ratio of N-acetyl-β-d-glucosaminidase to β-d-galactosidase, it was possible to distinguish clearly the patient with generalized gangliosidosis from the control group.
|Original language||English (US)|
|Number of pages||7|
|Journal||The Journal of laboratory and clinical medicine|
|State||Published - Nov 1 1969|
ASJC Scopus subject areas
- Pathology and Forensic Medicine