β-Adrenergic receptors in human trabecular meshwork. Identification and autoradiographic localization

Henry D Jampel, M. G. Lynch, R. H. Brown, M. J. Kuhar, E. B. De Souza

Research output: Contribution to journalArticle

Abstract

β-Adrenergic receptors were identified in slide-mounted sections of human trabecular meshwork by in vitro labeling, light microscopic autoradiography. Autoradiograms were generated after incubation of slide-mounted tissue sections with 125I-cyanopindolol, a selective high-affinity probe for β-adrenergic receptors. Experiments in which an excess of either unlabeled Practolol (β1 selective ligand) or Zinterol (β2 selective ligand) were included suggested that most of the receptors were of the β2 subtype. Data from displacement studies using increasing concentrations of the highly specific β1- and β2-adrenergic receptors antagonists, ICI-89,406 and ICI-118,551, respectively, confirmed the predominance of the β2-adrenergic receptors. Similar results obtained with cultured trabecular endothelial cells suggest that the β-adrenergic receptors may be associated with the endothelial cells of the trabecular meshwork in vivo. These results provide anatomic evidence for the hypothesis that β-adrenergic agents improve outflow by direct action on the trabecular meshwork and provide a rationale for the development of more selective β2-adrenergic agents to increase outflow facility.

Original languageEnglish (US)
Pages (from-to)772-779
Number of pages8
JournalInvestigative Ophthalmology and Visual Science
Volume28
Issue number5
StatePublished - 1987

Fingerprint

Trabecular Meshwork
Adrenergic Receptors
Adrenergic Agents
Endothelial Cells
Practolol
Ligands
Adrenergic Antagonists
Autoradiography
Light

ASJC Scopus subject areas

  • Ophthalmology

Cite this

β-Adrenergic receptors in human trabecular meshwork. Identification and autoradiographic localization. / Jampel, Henry D; Lynch, M. G.; Brown, R. H.; Kuhar, M. J.; De Souza, E. B.

In: Investigative Ophthalmology and Visual Science, Vol. 28, No. 5, 1987, p. 772-779.

Research output: Contribution to journalArticle

Jampel, Henry D ; Lynch, M. G. ; Brown, R. H. ; Kuhar, M. J. ; De Souza, E. B. / β-Adrenergic receptors in human trabecular meshwork. Identification and autoradiographic localization. In: Investigative Ophthalmology and Visual Science. 1987 ; Vol. 28, No. 5. pp. 772-779.
@article{edbe57e1b47c418ebb258d7328723326,
title = "β-Adrenergic receptors in human trabecular meshwork. Identification and autoradiographic localization",
abstract = "β-Adrenergic receptors were identified in slide-mounted sections of human trabecular meshwork by in vitro labeling, light microscopic autoradiography. Autoradiograms were generated after incubation of slide-mounted tissue sections with 125I-cyanopindolol, a selective high-affinity probe for β-adrenergic receptors. Experiments in which an excess of either unlabeled Practolol (β1 selective ligand) or Zinterol (β2 selective ligand) were included suggested that most of the receptors were of the β2 subtype. Data from displacement studies using increasing concentrations of the highly specific β1- and β2-adrenergic receptors antagonists, ICI-89,406 and ICI-118,551, respectively, confirmed the predominance of the β2-adrenergic receptors. Similar results obtained with cultured trabecular endothelial cells suggest that the β-adrenergic receptors may be associated with the endothelial cells of the trabecular meshwork in vivo. These results provide anatomic evidence for the hypothesis that β-adrenergic agents improve outflow by direct action on the trabecular meshwork and provide a rationale for the development of more selective β2-adrenergic agents to increase outflow facility.",
author = "Jampel, {Henry D} and Lynch, {M. G.} and Brown, {R. H.} and Kuhar, {M. J.} and {De Souza}, {E. B.}",
year = "1987",
language = "English (US)",
volume = "28",
pages = "772--779",
journal = "Investigative Ophthalmology and Visual Science",
issn = "0146-0404",
publisher = "Association for Research in Vision and Ophthalmology Inc.",
number = "5",

}

TY - JOUR

T1 - β-Adrenergic receptors in human trabecular meshwork. Identification and autoradiographic localization

AU - Jampel, Henry D

AU - Lynch, M. G.

AU - Brown, R. H.

AU - Kuhar, M. J.

AU - De Souza, E. B.

PY - 1987

Y1 - 1987

N2 - β-Adrenergic receptors were identified in slide-mounted sections of human trabecular meshwork by in vitro labeling, light microscopic autoradiography. Autoradiograms were generated after incubation of slide-mounted tissue sections with 125I-cyanopindolol, a selective high-affinity probe for β-adrenergic receptors. Experiments in which an excess of either unlabeled Practolol (β1 selective ligand) or Zinterol (β2 selective ligand) were included suggested that most of the receptors were of the β2 subtype. Data from displacement studies using increasing concentrations of the highly specific β1- and β2-adrenergic receptors antagonists, ICI-89,406 and ICI-118,551, respectively, confirmed the predominance of the β2-adrenergic receptors. Similar results obtained with cultured trabecular endothelial cells suggest that the β-adrenergic receptors may be associated with the endothelial cells of the trabecular meshwork in vivo. These results provide anatomic evidence for the hypothesis that β-adrenergic agents improve outflow by direct action on the trabecular meshwork and provide a rationale for the development of more selective β2-adrenergic agents to increase outflow facility.

AB - β-Adrenergic receptors were identified in slide-mounted sections of human trabecular meshwork by in vitro labeling, light microscopic autoradiography. Autoradiograms were generated after incubation of slide-mounted tissue sections with 125I-cyanopindolol, a selective high-affinity probe for β-adrenergic receptors. Experiments in which an excess of either unlabeled Practolol (β1 selective ligand) or Zinterol (β2 selective ligand) were included suggested that most of the receptors were of the β2 subtype. Data from displacement studies using increasing concentrations of the highly specific β1- and β2-adrenergic receptors antagonists, ICI-89,406 and ICI-118,551, respectively, confirmed the predominance of the β2-adrenergic receptors. Similar results obtained with cultured trabecular endothelial cells suggest that the β-adrenergic receptors may be associated with the endothelial cells of the trabecular meshwork in vivo. These results provide anatomic evidence for the hypothesis that β-adrenergic agents improve outflow by direct action on the trabecular meshwork and provide a rationale for the development of more selective β2-adrenergic agents to increase outflow facility.

UR - http://www.scopus.com/inward/record.url?scp=0023264316&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023264316&partnerID=8YFLogxK

M3 - Article

C2 - 3032837

AN - SCOPUS:0023264316

VL - 28

SP - 772

EP - 779

JO - Investigative Ophthalmology and Visual Science

JF - Investigative Ophthalmology and Visual Science

SN - 0146-0404

IS - 5

ER -