β-adrenergic modulation of Na-K pump activity in young and adult canine cardiac Purkinje fibers

Flavien Charpentier, Marianne J. Legato, Susan F. Steinberg, Ira S. Cohen, Michael R. Rosen

Research output: Contribution to journalArticle

Abstract

We used standard microelectrode techniques to study the developmental changes and β-adrenergic modulation of membrane potential and of Na-K pump activity in adult (>1 yr of age) and neonatal (2-10 days) canine Purkinje fibers. Isoproterenol (10-7 M) increased the rate of development and magnitude of pacing-induced hyperpolarization of adult fibers driven at a 1- s cycle length. This effect of isoproterenol was attenuated by treating dogs with pertussis toxin (PTX), (30 μg/kg). Other adult and neonatal fibers were superfused with a Tyrode solution containing Ba2+ 0.2 mM, Cs+ 2 mM, and 10-6 M verapamil, thus leading to depolarization and cessation of spontaneous activity. The Na-K pump was studied by alternating solutions containing [K] at 0 mM (inhibiting the pump) and 4 mM (reactivating the pump). Although the kinetics of the Na-K pump appeared faster in neonatal fibers than in adult fibers, measurement of cell surface-to-volume ratio compensated for the difference. We therefore conclude that 1) the apparent age-related changes in Na-K pump activity in canine Purkinje fibers in fact reflect cell surface-to-volume ratio and, 2) the β-adrenergic agonist- induced hyperpolarization in adults requires the presence of a PTX-sensitive G protein for its occurrence.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume271
Issue number2 40-2
StatePublished - Aug 1996
Externally publishedYes

Fingerprint

Purkinje Fibers
Pertussis Toxin
Isoproterenol
Adrenergic Agents
Canidae
Young Adult
Adrenergic Agonists
Microelectrodes
Verapamil
GTP-Binding Proteins
Membrane Potentials
Dogs
Tyrode's solution

Keywords

  • autonomic nervous system
  • G protein
  • isoproterenol
  • pertussis toxin

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

β-adrenergic modulation of Na-K pump activity in young and adult canine cardiac Purkinje fibers. / Charpentier, Flavien; Legato, Marianne J.; Steinberg, Susan F.; Cohen, Ira S.; Rosen, Michael R.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 271, No. 2 40-2, 08.1996.

Research output: Contribution to journalArticle

Charpentier, Flavien ; Legato, Marianne J. ; Steinberg, Susan F. ; Cohen, Ira S. ; Rosen, Michael R. / β-adrenergic modulation of Na-K pump activity in young and adult canine cardiac Purkinje fibers. In: American Journal of Physiology - Heart and Circulatory Physiology. 1996 ; Vol. 271, No. 2 40-2.
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