β-Adrenergic modulation of Na-K pump activity in young and adult canine cardiac Purkinje fibers

Flavien Charpentier, Marianne J. Legato, Susan F. Steinberg, Ira S. Cohen, Michael R. Rosen

Research output: Contribution to journalArticlepeer-review

Abstract

We used standard microelectrode techniques to study the developmental changes and β-adrenergic modulation of membrane potential and of Na-K pump activity in. adult (> 1 yr of age) and neonatal (2-10 days) canine Purkinje fibers. Isoproterenol (10-7 M) increased the rate of development and magnitude of pacing-induced hyperpolarization of adult fibers driven at a 1-s cycle length. This effect of isoproterenol was attenuated by treating dogs with pertussis toxin (PTX), (30 μg/kg). Other adult and neonatal fibers were superfused with a Tyrode solution containing Ba2+ 0.2 mM, Cs+ 2 mM, and 10-6 M verapamil, thus leading to depolarization and cessation of spontaneous activity. The Na-K pump was studied by alternating solutions containing [K] at 0 mM (inhibiting the pump) and 4 mM (reactivating the pump). Although the kinetics of the Na-K pump appeared faster in neonatal fibers than in adult fibers, measurement of cell surface-to-volume ratio compensated for the difference. We therefore conclude that 1) the apparent age-related changes in Na-K pump activity in canine Purkinje fibers in fact reflect cell surface-to-volume ratio and, 2) the β-adrenergic agonist-induced hyperpolarization in adults requires the presence of a PTX-sensitive G protein for its occurrence.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume40
Issue number2
StatePublished - Aug 1996
Externally publishedYes

Keywords

  • Autonomic nervous system
  • G protein
  • Isoproterenol
  • Pertussis toxin

ASJC Scopus subject areas

  • Physiology

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