α-melanocyte-stimulating hormone protects against mesenteric ischemia-reperfusion injury

Heitham T. Hassoun, Lei Zou, Frederick A. Moore, Rosemary A. Kozar, Norman W. Weisbrodt, Bruce C. Kone

Research output: Contribution to journalArticlepeer-review

Abstract

Mesenteric ischemia-reperfusion (I/R) injury to the intestine is a common and often devastating clinical occurrence for which there are few therapeutic options. α-Melanocyte-stimulating hormone (α-MSH) is a tridecapeptide released by the pituitary gland and immunocompetent cells that exerts antiinflammatory actions and abrogates postischemic injury to the kidneys and brainstem of rodents. To test the hypothesis that α-MSH would afford similar protection in the postischemic small intestine, we analyzed the effects of this peptide on intestinal transit, histology, myeloperoxidase activity, and nuclear factor-κB (NF-κB) activation after 45 min of superior mesenteric artery occlusion and ≤6 h of reperfusion. Rats subjected to I/R exhibited markedly depressed intestinal transit, histological evidence of severe injury to the ileum, increased myeloperoxidase activity in ileal cytoplasmic extracts, and biphasic activation of NF-κB in ileal nuclear extracts. In contrast, rats treated with α-MSH before I/R exhibited intestinal transit and histological injury scores comparable to those of sham-operated controls. In addition, the α-MSH-treated rats demonstrated less I/R-induced activation of intestinal NF-κB and myeloperoxidase activity after prolonged (6 h) reperfusion. We conclude that α-MSH significantly limits postischemic injury to the rat small intestine.

Original languageEnglish (US)
Pages (from-to)G1059-G1068
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume282
Issue number6 45-6
DOIs
StatePublished - 2002

Keywords

  • Ileus
  • Nuclear factor-κB
  • Small intestine
  • Transcription factor

ASJC Scopus subject areas

  • Physiology
  • Hepatology
  • Gastroenterology
  • Physiology (medical)

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