α-Ketoglutarate stimulates pendrin-dependent Cl- absorption in the mouse CCD through protein kinase C

Yoskaly Lazo-Fernandez, Paul A. Welling, Susan M. Wall

Research output: Contribution to journalArticle

Abstract

α-Ketoglutarate (α-KG) is a citric acid cycle intermediate and a glutamine catabolism product. It is also the natural ligand of 2-oxoglutarate receptor 1 (OXGR1), a Gq protein-coupled receptor expressed on the apical membrane of intercalated cells. In the cortical collecting duct (CCD), Cl-/HCO3 - exchange increases upon α-KG binding to the OXGR1. To determine the signaling pathway(s) by which α-KG stimulates Cl- absorption, we examined α-KG-stimulated Cl- absorption in isolated perfused mouse CCDs. α-KG increased electroneutral Cl- absorption in CCDs from wild-type mice but had no effect on Cl- absorption in pendrin knockout mice. Because Gq proteincoupled receptors activate PKC, we hypothesized that α-KG stimulates Cl- absorption through PKC. If so, PKC agonists should mimic, whereas PKC inhibitors should abolish, α-KG-stimulated Cl- absorption. Like α-KG, PKC agonist (phorbol-12,13-dibutyrate, 500 nM) application increased Cl- absorption in wild-type but not in pendrin null CCDs. Moreover, PKC inhibitors (2.5 mM GF109203X and 20 nM calphostin C), Ca2+ chelators (BAPTA, 10-20 µM), or PKC-α or -δ gene ablation eliminated α-KG-stimulated Cl- absorption. We have shown that STE20/SPS-1-related proline-alanine-rich protein kinase (SPAK) gene ablation increases urinary α-KG excretion, renal pendrin abundance, and CCD Cl- absorption. However, in SPAK null CCDs, Cl- absorption was not activated further by luminal α-KG application nor was Cl- absorption reduced with the PKC inhibitor GF109203. Thus SPAK gene ablation likely acts through a PKCindependent pathway to produce a chronic adaptive increase in pendrin function. In conclusion, α-KG stimulates pendrin-dependent Cl-/HCO3 - exchange through a mechanism dependent on PKC and Ca2+ that involves PKC-α and PKC-δ.

Original languageEnglish (US)
Pages (from-to)F7-F15
JournalAmerican Journal of Physiology - Renal Physiology
Volume315
Issue number1
DOIs
StatePublished - Jul 2018
Externally publishedYes

Fingerprint

Protein Kinase C
Proline
Alanine
Protein Kinases
Gq-G11 GTP-Binding Protein alpha Subunits
Genes
Phorbol 12,13-Dibutyrate
Citric Acid Cycle
Chelating Agents
Glutamine
Knockout Mice
Cell Membrane
Ligands

ASJC Scopus subject areas

  • Physiology
  • Urology

Cite this

α-Ketoglutarate stimulates pendrin-dependent Cl- absorption in the mouse CCD through protein kinase C. / Lazo-Fernandez, Yoskaly; Welling, Paul A.; Wall, Susan M.

In: American Journal of Physiology - Renal Physiology, Vol. 315, No. 1, 07.2018, p. F7-F15.

Research output: Contribution to journalArticle

@article{89edd08955ce45f0aeafdc0cbc8fe007,
title = "α-Ketoglutarate stimulates pendrin-dependent Cl- absorption in the mouse CCD through protein kinase C",
abstract = "α-Ketoglutarate (α-KG) is a citric acid cycle intermediate and a glutamine catabolism product. It is also the natural ligand of 2-oxoglutarate receptor 1 (OXGR1), a Gq protein-coupled receptor expressed on the apical membrane of intercalated cells. In the cortical collecting duct (CCD), Cl-/HCO3 - exchange increases upon α-KG binding to the OXGR1. To determine the signaling pathway(s) by which α-KG stimulates Cl- absorption, we examined α-KG-stimulated Cl- absorption in isolated perfused mouse CCDs. α-KG increased electroneutral Cl- absorption in CCDs from wild-type mice but had no effect on Cl- absorption in pendrin knockout mice. Because Gq proteincoupled receptors activate PKC, we hypothesized that α-KG stimulates Cl- absorption through PKC. If so, PKC agonists should mimic, whereas PKC inhibitors should abolish, α-KG-stimulated Cl- absorption. Like α-KG, PKC agonist (phorbol-12,13-dibutyrate, 500 nM) application increased Cl- absorption in wild-type but not in pendrin null CCDs. Moreover, PKC inhibitors (2.5 mM GF109203X and 20 nM calphostin C), Ca2+ chelators (BAPTA, 10-20 µM), or PKC-α or -δ gene ablation eliminated α-KG-stimulated Cl- absorption. We have shown that STE20/SPS-1-related proline-alanine-rich protein kinase (SPAK) gene ablation increases urinary α-KG excretion, renal pendrin abundance, and CCD Cl- absorption. However, in SPAK null CCDs, Cl- absorption was not activated further by luminal α-KG application nor was Cl- absorption reduced with the PKC inhibitor GF109203. Thus SPAK gene ablation likely acts through a PKCindependent pathway to produce a chronic adaptive increase in pendrin function. In conclusion, α-KG stimulates pendrin-dependent Cl-/HCO3 - exchange through a mechanism dependent on PKC and Ca2+ that involves PKC-α and PKC-δ.",
author = "Yoskaly Lazo-Fernandez and Welling, {Paul A.} and Wall, {Susan M.}",
year = "2018",
month = "7",
doi = "10.1152/ajprenal.00576.2017",
language = "English (US)",
volume = "315",
pages = "F7--F15",
journal = "American Journal of Physiology - Renal Physiology",
issn = "0363-6127",
number = "1",

}

TY - JOUR

T1 - α-Ketoglutarate stimulates pendrin-dependent Cl- absorption in the mouse CCD through protein kinase C

AU - Lazo-Fernandez, Yoskaly

AU - Welling, Paul A.

AU - Wall, Susan M.

PY - 2018/7

Y1 - 2018/7

N2 - α-Ketoglutarate (α-KG) is a citric acid cycle intermediate and a glutamine catabolism product. It is also the natural ligand of 2-oxoglutarate receptor 1 (OXGR1), a Gq protein-coupled receptor expressed on the apical membrane of intercalated cells. In the cortical collecting duct (CCD), Cl-/HCO3 - exchange increases upon α-KG binding to the OXGR1. To determine the signaling pathway(s) by which α-KG stimulates Cl- absorption, we examined α-KG-stimulated Cl- absorption in isolated perfused mouse CCDs. α-KG increased electroneutral Cl- absorption in CCDs from wild-type mice but had no effect on Cl- absorption in pendrin knockout mice. Because Gq proteincoupled receptors activate PKC, we hypothesized that α-KG stimulates Cl- absorption through PKC. If so, PKC agonists should mimic, whereas PKC inhibitors should abolish, α-KG-stimulated Cl- absorption. Like α-KG, PKC agonist (phorbol-12,13-dibutyrate, 500 nM) application increased Cl- absorption in wild-type but not in pendrin null CCDs. Moreover, PKC inhibitors (2.5 mM GF109203X and 20 nM calphostin C), Ca2+ chelators (BAPTA, 10-20 µM), or PKC-α or -δ gene ablation eliminated α-KG-stimulated Cl- absorption. We have shown that STE20/SPS-1-related proline-alanine-rich protein kinase (SPAK) gene ablation increases urinary α-KG excretion, renal pendrin abundance, and CCD Cl- absorption. However, in SPAK null CCDs, Cl- absorption was not activated further by luminal α-KG application nor was Cl- absorption reduced with the PKC inhibitor GF109203. Thus SPAK gene ablation likely acts through a PKCindependent pathway to produce a chronic adaptive increase in pendrin function. In conclusion, α-KG stimulates pendrin-dependent Cl-/HCO3 - exchange through a mechanism dependent on PKC and Ca2+ that involves PKC-α and PKC-δ.

AB - α-Ketoglutarate (α-KG) is a citric acid cycle intermediate and a glutamine catabolism product. It is also the natural ligand of 2-oxoglutarate receptor 1 (OXGR1), a Gq protein-coupled receptor expressed on the apical membrane of intercalated cells. In the cortical collecting duct (CCD), Cl-/HCO3 - exchange increases upon α-KG binding to the OXGR1. To determine the signaling pathway(s) by which α-KG stimulates Cl- absorption, we examined α-KG-stimulated Cl- absorption in isolated perfused mouse CCDs. α-KG increased electroneutral Cl- absorption in CCDs from wild-type mice but had no effect on Cl- absorption in pendrin knockout mice. Because Gq proteincoupled receptors activate PKC, we hypothesized that α-KG stimulates Cl- absorption through PKC. If so, PKC agonists should mimic, whereas PKC inhibitors should abolish, α-KG-stimulated Cl- absorption. Like α-KG, PKC agonist (phorbol-12,13-dibutyrate, 500 nM) application increased Cl- absorption in wild-type but not in pendrin null CCDs. Moreover, PKC inhibitors (2.5 mM GF109203X and 20 nM calphostin C), Ca2+ chelators (BAPTA, 10-20 µM), or PKC-α or -δ gene ablation eliminated α-KG-stimulated Cl- absorption. We have shown that STE20/SPS-1-related proline-alanine-rich protein kinase (SPAK) gene ablation increases urinary α-KG excretion, renal pendrin abundance, and CCD Cl- absorption. However, in SPAK null CCDs, Cl- absorption was not activated further by luminal α-KG application nor was Cl- absorption reduced with the PKC inhibitor GF109203. Thus SPAK gene ablation likely acts through a PKCindependent pathway to produce a chronic adaptive increase in pendrin function. In conclusion, α-KG stimulates pendrin-dependent Cl-/HCO3 - exchange through a mechanism dependent on PKC and Ca2+ that involves PKC-α and PKC-δ.

UR - http://www.scopus.com/inward/record.url?scp=85051112874&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85051112874&partnerID=8YFLogxK

U2 - 10.1152/ajprenal.00576.2017

DO - 10.1152/ajprenal.00576.2017

M3 - Article

C2 - 29412702

AN - SCOPUS:85051112874

VL - 315

SP - F7-F15

JO - American Journal of Physiology - Renal Physiology

JF - American Journal of Physiology - Renal Physiology

SN - 0363-6127

IS - 1

ER -