TY - JOUR
T1 - α-Endosulfine, a potential regulator of insulin secretion, is required for adult tissue growth control in Drosophila
AU - Drummond-Barbosa, Daniela
AU - Spradling, Allan C.
N1 - Funding Information:
We thank Laura Buttitta for plasmid rescuing the fs(3)00003 P-element insertion. We are grateful to Mark Brown for anti-DILP antibodies. A.C.S. is an Investigator of the Howard Hughes Medical Institute. This work was partially supported by the VUMC Discovery Grant Program.
PY - 2004/2/15
Y1 - 2004/2/15
N2 - α-Endosulfine is a small protein that has been proposed to regulate ion channel activity and insulin secretion, but in vivo studies have been lacking. We have previously established the Drosophila ovary as a model system in which to study adult tissue growth regulation, and demonstrated a role of the insulin pathway in the proliferative response of ovarian cells to nutritional changes. Here, we find that the Drosophila α-endosulfine (dendos) gene, whose protein is expressed in germline and somatic cells of the ovary, as well as in the brain and certain regions of the intestine, is also required for this response. This requirement is non-cell autonomous, which is consistent with a role of dendos in secretion of Drosophila insulin-like peptides (DILPs), required for the proliferative response to nutritional changes. Our results show that dendos is also required for a distinct process in oogenesis, namely, the osmotic regulation of stage 14 oocytes, and that this requirement is cell autonomous, consistent with the role in ion channel regulation suggested by studies of the mammalian homologues.
AB - α-Endosulfine is a small protein that has been proposed to regulate ion channel activity and insulin secretion, but in vivo studies have been lacking. We have previously established the Drosophila ovary as a model system in which to study adult tissue growth regulation, and demonstrated a role of the insulin pathway in the proliferative response of ovarian cells to nutritional changes. Here, we find that the Drosophila α-endosulfine (dendos) gene, whose protein is expressed in germline and somatic cells of the ovary, as well as in the brain and certain regions of the intestine, is also required for this response. This requirement is non-cell autonomous, which is consistent with a role of dendos in secretion of Drosophila insulin-like peptides (DILPs), required for the proliferative response to nutritional changes. Our results show that dendos is also required for a distinct process in oogenesis, namely, the osmotic regulation of stage 14 oocytes, and that this requirement is cell autonomous, consistent with the role in ion channel regulation suggested by studies of the mammalian homologues.
KW - Insulin
KW - Oogenesis
KW - Tissue growth
KW - dendos
KW - α-Endosulfine
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U2 - 10.1016/j.ydbio.2003.10.028
DO - 10.1016/j.ydbio.2003.10.028
M3 - Article
C2 - 14738879
AN - SCOPUS:0347131105
SN - 0012-1606
VL - 266
SP - 310
EP - 321
JO - Developmental biology
JF - Developmental biology
IS - 2
ER -