α-Catenin uses a novel mechanism to activate vinculin

Xiao Peng, Jessica L. Maiers, Dilshad Choudhury, Susan W. Craig, Kris A. DeMali

Research output: Contribution to journalArticle

Abstract

Vinculin, an actin-binding protein, is emerging as an important regulator of adherens junctions. In focal-adhesions, vinculin is activated by simultaneous binding of talin to its head domain and actin filaments to its tail domain. Talin is not present in adherens junctions. Consequently, the identity of the ligand that activates vinculin in cell-cell junctions is not known. Here we show that in the presence of F-actin, α-catenin, a cytoplasmic component of the cadherin adhesion complex, activates vinculin. Direct binding of α-catenin to vinculin is critical for this event because a point mutant (α-catenin L344P) lacking high affinity binding does not activate vinculin. Furthermore, unlike all known vinculin activators, α-catenin binds to and activates vinculin independently of an A50I substitution in the vinculin head, a mutation that inhibits vinculin binding to talin and IpaA. Collectively, these data suggest that α-catenin employs a novel mechanism to activate vinculin and may explain how vinculin is differentially recruited and/or activated in cellcell and cell-matrix adhesions.

Original languageEnglish (US)
Pages (from-to)7728-7737
Number of pages10
JournalJournal of Biological Chemistry
Volume287
Issue number10
DOIs
StatePublished - Mar 2 2012

Fingerprint

Vinculin
Catenins
Talin
Adherens Junctions
Adhesion
Actins
Cell-Matrix Junctions
Microfilament Proteins
Focal Adhesions
Intercellular Junctions
Cadherins
Actin Cytoskeleton

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Peng, X., Maiers, J. L., Choudhury, D., Craig, S. W., & DeMali, K. A. (2012). α-Catenin uses a novel mechanism to activate vinculin. Journal of Biological Chemistry, 287(10), 7728-7737. https://doi.org/10.1074/jbc.M111.297481

α-Catenin uses a novel mechanism to activate vinculin. / Peng, Xiao; Maiers, Jessica L.; Choudhury, Dilshad; Craig, Susan W.; DeMali, Kris A.

In: Journal of Biological Chemistry, Vol. 287, No. 10, 02.03.2012, p. 7728-7737.

Research output: Contribution to journalArticle

Peng, X, Maiers, JL, Choudhury, D, Craig, SW & DeMali, KA 2012, 'α-Catenin uses a novel mechanism to activate vinculin', Journal of Biological Chemistry, vol. 287, no. 10, pp. 7728-7737. https://doi.org/10.1074/jbc.M111.297481
Peng X, Maiers JL, Choudhury D, Craig SW, DeMali KA. α-Catenin uses a novel mechanism to activate vinculin. Journal of Biological Chemistry. 2012 Mar 2;287(10):7728-7737. https://doi.org/10.1074/jbc.M111.297481
Peng, Xiao ; Maiers, Jessica L. ; Choudhury, Dilshad ; Craig, Susan W. ; DeMali, Kris A. / α-Catenin uses a novel mechanism to activate vinculin. In: Journal of Biological Chemistry. 2012 ; Vol. 287, No. 10. pp. 7728-7737.
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