TY - JOUR
T1 - γδ T cells contribute to immunity against the liver stages of malaria in αβ T-cell-deficient mice
AU - Tsuji, Moriya
AU - Mombaerts, Peter
AU - Lefrancois, Leo
AU - Nussenzweig, Ruth S.
AU - Zavala, Fidel
AU - Tonegawa, Susumu
PY - 1994/1/4
Y1 - 1994/1/4
N2 - The functional role of γδ T cells (expressing the γδ heterodimeric T- cell receptor for antigen) in infectious diseases remains largely unknown. We have therefore attempted to define the possible role of these T cells in the immune response against the various developmental stages of malaria parasites. For this purpose, we monitored the immune response and the development of liver and blood stages of Plasmodium yoelii, a rodent malaria parasite, in immunized and nonimmunized αβ T-cell-deficient and γδ T- cell-deficient mice. Immunization of αβ T-cell-deficient mice with irradiated sporozoites induced an immune response that significantly inhibited the development of the parasite's liver stages. This inhibitory immune response was abolished by an antibody-mediated transient in vivo depletion of γδ T cells. Two γδ T-cell clones were derived from malaria- immunized αβ T-cell-deficient mice. The adoptive transfer of one of these γδ T-cell clones to normal mice inhibited the development of liver stages, following sporozoite inoculation. These results provide evidence for γδ T- cell-mediated protective immunity against parasites, in the absence of αβ T cells. As for the blood phase of the infection, both normal mice and γδ T- cell-deficient mice cleared the blood stages of the nonlethal strain of P. yoelii, while αβ T-cell-deficient mice failed to control the parasitemia.
AB - The functional role of γδ T cells (expressing the γδ heterodimeric T- cell receptor for antigen) in infectious diseases remains largely unknown. We have therefore attempted to define the possible role of these T cells in the immune response against the various developmental stages of malaria parasites. For this purpose, we monitored the immune response and the development of liver and blood stages of Plasmodium yoelii, a rodent malaria parasite, in immunized and nonimmunized αβ T-cell-deficient and γδ T- cell-deficient mice. Immunization of αβ T-cell-deficient mice with irradiated sporozoites induced an immune response that significantly inhibited the development of the parasite's liver stages. This inhibitory immune response was abolished by an antibody-mediated transient in vivo depletion of γδ T cells. Two γδ T-cell clones were derived from malaria- immunized αβ T-cell-deficient mice. The adoptive transfer of one of these γδ T-cell clones to normal mice inhibited the development of liver stages, following sporozoite inoculation. These results provide evidence for γδ T- cell-mediated protective immunity against parasites, in the absence of αβ T cells. As for the blood phase of the infection, both normal mice and γδ T- cell-deficient mice cleared the blood stages of the nonlethal strain of P. yoelii, while αβ T-cell-deficient mice failed to control the parasitemia.
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U2 - 10.1073/pnas.91.1.345
DO - 10.1073/pnas.91.1.345
M3 - Article
C2 - 8278391
AN - SCOPUS:0028174363
SN - 0027-8424
VL - 91
SP - 345
EP - 349
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 1
ER -