William Agnew

Professor

1975 …2007
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Research Output 1975 2007

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Article
2007

Multivariate analysis and visualization of splicing correlations in single-gene transcriptomes

Emerick, M. C., Parmigiani, G. & Agnew, W. S. 2007 In : BMC Bioinformatics. 8, 16

Research output: Contribution to journalArticle

Multivariate Analysis
Transcriptome
Visualization
Genes
Gene
2006

A profile of alternative RNA splicing and transcript variation of CACNA1H, a human T-channel gene candidate for idiopathic generalized epilepsies

Zhong, X., Liu, J. R., Kyle, J. W., Hanck, D. A. & Agnew, W. S. May 2006 In : Human Molecular Genetics. 15, 9, p. 1497-1512 16 p.

Research output: Contribution to journalArticle

Alternative Splicing
Missense Mutation
Mutation
Absence Epilepsy
Genes

Profiling the array of Cav3.1 variants from the human T-type calcium channel gene CACNA1G: Alternative structures, developmental expression, and biophysical variations

Emerick, M. C., Stein, R., Kunze, R., McNulty, M. M., Regan, M. R., Hanck, D. A. & Agnew, W. S. Aug 1 2006 In : Proteins. 64, 2, p. 320-342 23 p.

Research output: Contribution to journalArticle

T-Type Calcium Channels
Alternative Splicing
Genes
Biophysics
Polyadenylation
2005
Inositol 1,4,5-Trisphosphate Receptors
Transcriptome
RNA
Genes
Tissue
2002

Systematic identification of splice variants in human P/Q-type channel α,2.1 subunits: Implications for current density and Ca2+-dependent inactivation

Soong, T. W., DeMaria, C. D., Alvania, R. S., Zweifel, L. S., Cheng Liang, M., Mittman, S., Agnew, W. S. & Yue, D. T. Dec 1 2002 In : Journal of Neuroscience. 22, 23, p. 10142-10152 11 p.

Research output: Contribution to journalArticle

Alternative Splicing
Calcium Channels
Sequence Analysis
Exons
Brain
2000

Full-length single-gene cDNA libraries: Applications in splice variant analysis

Regan, M. R., Emerick, M. C. & Agnew, W. S. Nov 15 2000 In : Analytical Biochemistry. 286, 2, p. 265-276 12 p.

Research output: Contribution to journalArticle

Gene Library
Exons
Genes
Clone Cells
Transcription
1999

Structure and alternative splicing of the gene encoding α(1G), a human brain T calcium channel α1 subunit

Mittman, S., Guo, J. & Agnew, W. S. Oct 29 1999 In : Neuroscience Letters. 274, 3, p. 143-146 4 p.

Research output: Contribution to journalArticle

Alternative Splicing
Calcium Channels
Exons
Brain
Inteins

Structure and alternative splicing of the gene encoding α(1I), a human brain T calcium channel α1 subunit

Mittman, S., Guo, J., Emerick, M. C. & Agnew, W. S. Jul 16 1999 In : Neuroscience Letters. 269, 3, p. 121-124 4 p.

Research output: Contribution to journalArticle

Alternative Splicing
Calcium Channels
Exons
Brain
Genes
1997

Mechanism of modulation of the voltage-gated skeletal and cardiac muscle sodium channels by fatty acids

Bendahhou, S., Cummins, T. R. & Agnew, W. S. Feb 1997 In : American Journal of Physiology - Cell Physiology. 272, 2 41-2

Research output: Contribution to journalArticle

Sodium Channels
Oleic Acids
Arachidonic Acids
Muscle
Myocardium

The type 1 inositol 1,4,5-trisphosphate receptor gene is altered in the opisthotonos mouse

Street, V. A., Bosma, M. M., Demas, V. P., Regan, M. R., Lin, D. D., Robinson, L. C., Agnew, W. S. & Tempel, B. L. 1997 In : Journal of Neuroscience. 17, 2, p. 635-645 11 p.

Research output: Contribution to journalArticle

Inositol 1,4,5-Trisphosphate Receptors
Quisqualic Acid
Calcium
Homozygote
Genes
1996
Lipoxygenase
Metabolic Networks and Pathways
Unsaturated Fatty Acids
Fatty Acids
Chemical activation
1995
Nuclear Fusion
Inositol 1,4,5-Trisphosphate Receptors
Antibodies
Ligand-Gated Ion Channels
Inositol 1,4,5-Trisphosphate

Serine-1321-independent regulation of the μ1 adult skeletal muscle Na+ channel by protein kinase C

Bendahhou, S., Cummins, T. R., Potts, J. F., Tong, J. & Agnew, W. S. Dec 19 1995 In : Proceedings of the National Academy of Sciences of the United States of America. 92, 26, p. 12003-12007 5 p.

Research output: Contribution to journalArticle

Serine
Protein Kinase C
Skeletal Muscle
Cyclic AMP-Dependent Protein Kinases
Protein Isoforms
1994

A simplified method for single-cell RT-PCR that can detect and distinguish genomic DNA and mRNA transcripts

Tong, J., Bendahhou, S., Chen, H. & Agnew, W. S. Aug 11 1994 In : Nucleic Acids Research. 22, 15, p. 3253-3254 2 p.

Research output: Contribution to journalArticle

Polymerase Chain Reaction
Deoxyribonucleases
Actin
Polymerase chain reaction
RNA-Directed DNA Polymerase

The μI skeletal muscle sodium channel: Mutation E403Q eliminates sensitivity to tetrodotoxin but not to μ-conotoxins GIIIA and GIIIB

Stephan, M. M., Potts, J. F. & Agnew, W. S. Jan 1994 In : Journal of Membrane Biology. 137, 1, p. 1-8 8 p.

Research output: Contribution to journalArticle

Saxitoxin
Sodium Channels
Tetrodotoxin
Skeletal Muscle
Mutation
1993

A Glial-Specific Voltage-Sensitive Na Channel Gene Maps Close to Clustered Genes for Neuronal Isoforms on Mouse Chromosome 2

Potts, J. F., Regan, M. R., Rochelle, J. M., Seldin, M. F. & Agnew, W. S. Nov 30 1993 In : Biochemical and Biophysical Research Communications. 197, 1, p. 100-104 5 p.

Research output: Contribution to journalArticle

Chromosomes, Human, Pair 2
Chromosomes
Neuroglia
Protein Isoforms
Genes

A Single B1 Subunit Mapped to Mouse Chromosome 7 May Be a Common Component of Na Channel Isoforms from Brain, Skeletal Muscle and Heart

Tong, J. F., Potts, J. F., Rochelle, J. M., Seldin, M. F. & Agnew, W. S. Sep 15 1993 In : Biochemical and Biophysical Research Communications. 195, 2, p. 679-685 7 p.

Research output: Contribution to journalArticle

Chromosomes, Human, Pair 7
Chromosomes
Muscle
Brain
Protein Isoforms

Functional consequences of a Na+ channel mutation causing hyperkalemic periodic paralysis

Cummins, T. R., Zhou, J., Sigworth, F. J., Ukomadu, C., Stephan, M., Ptáčk, L. J. & Agnew, W. S. 1993 In : Neuron. 10, 4, p. 667-678 12 p.

Research output: Contribution to journalArticle

Hyperkalemic Periodic Paralysis
Mutation
Muscles
Inborn Genetic Diseases
Pedigree

Regulation of the eel electroplax na channel and phosphorylation of residues on amino- and carboxyl-terminal domains by cAMP-dependent protein kinase

Emerick, M. C., Shenkel, S. & Agnew, W. S. 1993 In : Biochemistry®. 32, 36, p. 9435-9444 10 p.

Research output: Contribution to journalArticle

Eels
Phosphorylation
Cyclic AMP-Dependent Protein Kinases
Serine
Threonine
1992

Assay and purification of neuronal receptors for inositol 1,4,5- trisphosphate

Hingorani, S. R. & Agnew, W. S. 1992 In : Methods in Enzymology. 207, p. 573-591 19 p.

Research output: Contribution to journalArticle

Inositol 1,4,5-Trisphosphate Receptors
Ligands
Intracellular Membranes
Ryanodine Receptor Calcium Release Channel
Inositol 1,4,5-Trisphosphate

μl Na+ channels expressed transiently in human embryonic kidney cells: Biochemical and biophysical properties

Ukomadu, C., Zhou, J., Sigworth, F. J. & Agnew, W. S. 1992 In : Neuron. 8, 4, p. 663-676 14 p.

Research output: Contribution to journalArticle

Hyperkalemic Periodic Paralysis
Kidney
Glycopeptides
HEK293 Cells
Second Messenger Systems
1991

Analysis in a large hyperkalemic periodic paralysis pedigree supports tight linkage to a sodium channel locus

Ptacek, L. J., Tyler, F., Trimmer, J. S., Agnew, W. S. & Leppert, M. Aug 1991 In : American Journal of Human Genetics. 49, 2, p. 378-382 5 p.

Research output: Contribution to journalArticle

Hyperkalemic Periodic Paralysis
Sodium Channels
Pedigree
Genes
Voltage-Gated Sodium Channels

A rapid ion-exchange assay for detergent-solubilized inositol 1,4,5-trisphosphate receptors

Hingorani, S. R. & Agnew, W. S. 1991 In : Analytical Biochemistry. 194, 1, p. 204-213 10 p.

Research output: Contribution to journalArticle

Inositol 1,4,5-Trisphosphate Receptors
Ion Exchange
Detergents
Assays
Ion exchange

Multiple gating modes and the effect of modulating factors on the μI sodium channel

Zhou, J., Potts, J. F., Trimmer, J. S., Agnew, W. S. & Sigworth, F. J. 1991 In : Neuron. 7, 5, p. 775-785 11 p.

Research output: Contribution to journalArticle

Sodium Channels
Skeletal Muscle
Hyperkalemic Periodic Paralysis
Complementary RNA
Messenger RNA

Paramyotonia congenita and hyperkalemic periodic paralysis map to the same sodium-channel gene locus

Ptacek, L. J., Trimmer, J. S., Agnew, W. S., Roberts, J. W., Petajan, J. H. & Leppert, M. Oct 1991 In : American Journal of Human Genetics. 49, 4, p. 851-854 4 p.

Research output: Contribution to journalArticle

Hyperkalemic Periodic Paralysis
Myotonic Disorders
Sodium Channels
Genes
Penetrance

Voltage-sensitive Na+ channels: motifs, modes and modulation

Stephan, M. & Agnew, W. S. 1991 In : Current Opinion in Cell Biology. 3, 4, p. 676-684 9 p.

Research output: Contribution to journalArticle

Ions

Voltage-sensitive sodium channels: Agents that perturb inactivation gating

Agnew, W. S., Cooper, E. C., Shenkel, S., Correa, A. M., James, W. M., Ukomadu, C. & Tomiko, S. A. 1991 In : Annals of the New York Academy of Sciences. 625, p. 200-223 24 p.

Research output: Contribution to journalArticle

Sodium Channels
Chemical activation
Eels
Electric potential
Lidocaine
1990

Regulation of muscle sodium channel transcripts during development and in response to denervation

Trimmer, J. S., Cooperman, S. S., Agnew, W. S. & Mandel, G. 1990 In : Developmental Biology. 142, 2, p. 360-367 8 p.

Research output: Contribution to journalArticle

Sodium Channels
Denervation
Tetrodotoxin
Muscles
Skeletal Muscle

Voltage activation of purified eel sodium channels reconstituted into artificial liposomes

Correa, A. M., Bezanilla, F. & Agnew, W. S. 1990 In : Biochemistry®. 29, 26, p. 6230-6240 11 p.

Research output: Contribution to journalArticle

Electrophorus
Eels
Sodium Channels
Depolarization
Liposomes
1989

Affinity purification of the voltage-sensitive sodium channel from electroplax with resins selective for sialic acid

James, W. M., Emerick, M. C. & Agnew, W. S. 1989 In : Biochemistry®. 28, 14, p. 6001-6009 9 p.

Research output: Contribution to journalArticle

Sodium Channels
N-Acetylneuraminic Acid
Purification
Immunoglobulin M
Resins
Electric Organ
Eels
sodium channels
Sodium Channels
eel

Cloning of the SR foot

Agnew, W. S. 1989 In : Nature. 339, 6224, p. 422-423 2 p.

Research output: Contribution to journalArticle

Calcium Channel Blockers
Nicotinic Receptors
Sarcoplasmic Reticulum
Molecular Cloning
Calcium Channels
Electrophorus
Phosphorylation
Sodium Channels
Adenosine
Protein Kinases

Molecular diversity of voltage-sensitive Na channels

Trimmer, J. S. & Agnew, W. S. 1989 In : Annual Review of Physiology. 51, p. 401-418 18 p.

Research output: Contribution to journalArticle

Saxitoxin
Sodium Channels
Chromosome Mapping
Electrophysiology
Schwann Cells

Primary structure and functional expression of a mammalian skeletal muscle sodium channel

Trimmer, J. S., Cooperman, S. S., Tomiko, S. A., Zhou, J., Crean, S. M., Boyle, M. B., Kalen, R. G., Sheng, Z., Barchi, R. L., Sigworth, F. J., Goodman, R. H., Agnew, W. S. & Mandel, G. 1989 In : Neuron. 3, 1, p. 33-49 17 p.

Research output: Contribution to journalArticle

Sodium Channels
Skeletal Muscle
Conotoxins
Amino Acids
Eels

Purified, modified eel sodium channels are active in planar bilayers in the absence of activating neurotoxins

Shenkel, S., Cooper, E. C., James, W., Agnew, W. S. & Sigworth, F. J. 1989 In : Proceedings of the National Academy of Sciences of the United States of America. 86, 23, p. 9592-9596 5 p.

Research output: Contribution to journalArticle

Eels
Sodium Channels
Neurotoxins
Batrachotoxins
Sodium
Bromosuccinimide
Eels
Sodium Channels
Lidocaine
Permeability
1988

A Rosetta stone for K channels

Agnew, W. S. 1988 In : Nature. 331, 6152, p. 114-115 2 p.

Research output: Contribution to journalArticle

RNA Splicing
Chromosome Mapping
Ion Channels
Drosophila
Potassium

Chapter 17 Voltage-Sensitive Sodium Channels: Molecular Structure and Function

Agnew, W. S., Cooper, E. C., James, W. M., Tomiko, S. A., Rosenberg, R. L., Emerick, M. C., Correa, A. M. & Zhou, J. Y. 1988 In : Current Topics in Membranes and Transport. 33, C, p. 329-365 37 p.

Research output: Contribution to journalArticle

Sodium Channels
Molecular Structure
Neurotoxins
Site-Directed Mutagenesis
Carrier Proteins

Dual roles for DHP receptors in excitation-contraction coupling?

Agnew, W. S. 1988 In : Nature. 328, 6128, p. 297 1 p.

Research output: Contribution to journalArticle

Excitation Contraction Coupling
Calcium Channel Blockers
Nicotinic Receptors
Calcium Channels
Muscle Contraction

Fusion of native or reconstituted membranes to liposomes, optimized for single channel recording.

Correa, A. M. & Agnew, W. S. Sep 1988 In : Biophysical Journal. 54, 3, p. 569-575 7 p.

Research output: Contribution to journalArticle

Liposomes
Eels
Membranes
Batrachotoxins
Saxitoxin

Preface

Agnew, W. S., Claudio, T. & Sigworth, F. J. 1988 In : Current Topics in Membranes and Transport. 33, C

Research output: Contribution to journalArticle

Proteins that bridge the gap

Agnew, W. S. 1988 In : Nature. 334, 6180, p. 299-300 2 p.

Research output: Contribution to journalArticle

Ryanodine Receptor Calcium Release Channel
Nicotinic Receptors
Cholinergic Receptors
Calcium Channels
Muscle Contraction
1987

Hydrodynamic Properties of the Chromaffin Granule Hydrogen Ion Pumping Adenosinetriphosphatase

Dean, G. E., Rudnic, G., Agnew, W. & Nelson, P. J. 1987 In : Biochemistry. 26, 8, p. 2301-2305 5 p.

Research output: Contribution to journalArticle

Chromaffin Granules
Hydrodynamics
Detergents
Adenosine Triphosphatases
Protons

Molecular Weight and Hydrodynamic Properties of the Chromaffin Granule ATPase

DEAN, GARY. E., NELSON, PAMLEA. J., AGNEW, WILLIAM. S. & RUDNICK, GARY. 1987 In : Annals of the New York Academy of Sciences. 493, 1, p. 268-269 2 p.

Research output: Contribution to journalArticle

Electrophorus
Oligosaccharides
Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase
Neuraminidase
Electric potential
Electrophorus
Sodium Channels
Liposomes
Permeability
Neurotoxins
1986

Fluorescence Assay for Neurotoxin-Modulated Ion Transport by the Reconstituted Voltage-Activated Sodium Channel Isolated from Eel Electric Organ

Tomiko, S. A., Rosenberg, R. L., Emerick, M. C. & Agnew, W. S. 1986 In : Biochemistry. 25, 8, p. 2162-2174 13 p.

Research output: Contribution to journalArticle

Batrachotoxins
Electric Organ
Veratridine
Eels
Sodium Channels

Neurochemistry: Lessons from large molecules

Agnew, W. S. 1986 In : Nature. 322, 6082, p. 770-771 2 p.

Research output: Contribution to journalArticle

Neurochemistry
Protein Conformation
Ion Channels
Amino Acid Sequence
Glycoproteins

The structure and function of the voltage-sensitive Na channel

Agnew, W. S., Tomiko, S. A. & Rosenberg, R. L. 1986 In : Annals of the New York Academy of Sciences. Vol. 479, p. 238-256 19 p.

Research output: Contribution to journalArticle

Electric Organ
Ion Channels
Membrane Proteins
Molecular Weight
Sodium