Research Output 1989 2018

2018

Conditional gene knockout and reconstitution in human iPSCs with an inducible Cas9 system

Wu, M., Liu, S., Gao, Y., Bai, H., Machairaki, V., Li, G., Chen, T. & Cheng, L. May 1 2018 In : Stem Cell Research. 29, p. 6-14 9 p.

Research output: Contribution to journalArticle

Induced Pluripotent Stem Cells
Gene Knockout Techniques
Guide RNA
Gene Order
Transgenes

Human NOTCH4 is a key target of RUNX1 in megakaryocytic differentiation

Li, Y., Jin, C., Bai, H., Gao, Y., Sun, S., Chen, L., Qin, L., Liu, P. P., Cheng, L. & Wang, Q. F. Jan 11 2018 In : Blood. 131, 2, p. 191-201 11 p.

Research output: Contribution to journalArticle

Induced Pluripotent Stem Cells
Megakaryocytes
Platelets
Stem cells
Blood Platelets
2017

A hypomorphic PIGA gene mutation causes severe defects in neuron development and susceptibility to complement-mediated toxicity in a human iPSC model

Yuan, X., Li, Z., Baines, A. C., Gavriilaki, E., Ye, Z., Wen, Z., Braunstein, E. M., Biesecker, L. G., Cheng, L., Dong, X. & Brodsky, R. A. Apr 1 2017 In : PLoS ONE. 12, 4, e0174074

Research output: Contribution to journalArticle

Glycosylphosphatidylinositols
Neurons
Toxicity
complement
neurodevelopment

A Universal Approach to Correct Various HBB Gene Mutations in Human Stem Cells for Gene Therapy of Beta-Thalassemia and Sickle Cell Disease

Cai, L., Bai, H., Mahairaki, V., Gao, Y., He, C., Wen, Y., Jin, Y. C., Wang, Y., Pan, R. L., Qasba, A., Ye, Z. & Cheng, L. Jan 1 2017 (Accepted/In press) In : Stem Cells Translational Medicine.

Research output: Contribution to journalArticle

beta-Thalassemia
Sickle Cell Anemia
Cell- and Tissue-Based Therapy
Genetic Therapy
Stem Cells

Generation of human iPSCs from an essential thrombocythemia patient carrying a V501L mutation in the MPL gene

Liu, S., Ye, Z., Gao, Y., He, C., Williams, D. W., Moliterno, A., Spivak, J., Huang, H. & Cheng, L. Jan 1 2017 In : Stem Cell Research. 18, p. 57-59 3 p.

Research output: Contribution to journalArticle

Essential Thrombocythemia
Induced Pluripotent Stem Cells
Mutation
Thrombopoietin Receptors
Genes
megakaryocytes
Induced Pluripotent Stem Cells
Megakaryocytes
Stem cells
transcriptomics

Isolation and characterization of extracellular vesicles in stem cell-related studies

Zhao, Z., Muth, D. C., Mahairaki, V., Cheng, L. & Witwer, K. W. 2017 Neuromethods. Humana Press Inc., Vol. 126, p. 205-223 19 p. (Neuromethods; vol. 126)

Research output: Chapter in Book/Report/Conference proceedingChapter

Stem cells
Stem Cells
Centrifugation
Ultracentrifugation
Purification
2016

A germline ERBB3 variant is a candidate for predisposition to erythroid MDS/erythroleukemia

Braunstein, E. M., Li, R., Sobreira, N., Marosy, B., Hetrick, K., Doheny, K., Gocke, C. D., Valle, D., Brodsky, R. A. & Cheng, L. Jul 15 2016 (Accepted/In press) In : Leukemia.

Research output: Contribution to journalArticle

A method for genome editing in human pluripotent stem cells

Smith, C., Ye, Z. & Cheng, L. Apr 1 2016 In : Cold Spring Harbor Protocols. 2016, 4, p. 385-390 6 p.

Research output: Contribution to journalArticle

Pluripotent Stem Cells
Stem cells
Genes
Regenerative Medicine
Research Personnel

Definitive Hematopoietic Multipotent Progenitor Cells Are Transiently Generated From Hemogenic Endothelial Cells in Human Pluripotent Stem Cells

Bai, H., Liu, Y., Xie, Y., Hoyle, D. L., Brodsky, R. A., Cheng, L., Cheng, T. & Wang, Z. Z. May 1 2016 In : Journal of Cellular Physiology. 231, 5, p. 1065-1076 12 p.

Research output: Contribution to journalArticle

Hemangioblasts
Pluripotent Stem Cells
Endothelial cells
Hematopoietic Stem Cells
Stem cells

Erratum to: Questions about NgAgo

Burgess, S., Cheng, L., Gu, F., Huang, J., Huang, Z., Lin, S., Li, J., Li, W., Qin, W., Sun, Y., Songyang, Z., Wei, W., Wu, Q., Wang, H., Wang, X., Xiong, J. W., Xi, J., Yang, H., Zhou, B. & Zhang, B. Dec 2 2016 (Accepted/In press) In : Protein and Cell. p. 1 1 p.

Research output: Contribution to journalArticle

HEK293 Cells
Transfection
Publications
Proteins

Genome editing in human pluripotent stem cells

Smith, C., Ye, Z. & Cheng, L. Apr 1 2016 In : Cold Spring Harbor Protocols. 2016, 4, p. 316-319 4 p.

Research output: Contribution to journalArticle

Pluripotent Stem Cells
Stem cells
Genes
Clustered Regularly Interspaced Short Palindromic Repeats
Guide RNA

Integration-free erythroblast-derived human induced pluripotent stem cells (iPSCs) from an individual with Ataxia-Telangiectasia (A-T)

Bhatt, N., Ghosh, R., Roy, S., Gao, Y., Armanios, M., Cheng, L. & Franco, S. Sep 1 2016 In : Stem Cell Research. 17, 2, p. 205-207 3 p.

Research output: Contribution to journalArticle

Induced Pluripotent Stem Cells
Ataxia Telangiectasia
Erythroblasts
Erythroid Cells
Mutation

Questions about NgAgo

Burgess, S., Cheng, L., Gu, F., Huang, J., Huang, Z., Lin, S., Li, J., Li, W., Qin, W., Sun, Y., Songyang, Z., Wei, W., Wu, Q., Wang, H., Wang, X., Xiong, J. W., Xi, J., Yang, H., Zhou, B. & Zhang, B. Nov 15 2016 (Accepted/In press) In : Protein and Cell. p. 1-3 3 p.

Research output: Contribution to journalArticle

Robust reprogramming of Ataxia-Telangiectasia patient and carrier erythroid cells to induced pluripotent stem cells

Bhatt, N., Ghosh, R., Roy, S., Gao, Y., Armanios, M., Cheng, L. & Franco, S. Sep 1 2016 In : Stem Cell Research. 17, 2, p. 296-305 10 p.

Research output: Contribution to journalArticle

Induced Pluripotent Stem Cells
Ataxia Telangiectasia
Erythroid Cells
Haploinsufficiency
Teratoma
2015

A facile method to establish human induced pluripotent stem cells from adult blood cells under feeder-free and xeno-free culture conditions: A clinically compliant approach

Chou, B. K. , Chou, B. K. , Gu, H. , Gu, H. , Gu, H. , Gao, Y. , Gao, Y. , Dowey, S. N. , Dowey, S. N. , Wang, Y. , Wang, Y. , Shi, J. , Shi, J. , Li, Y. , Li, Y. , Li, Y. , Ye, Z. , Ye, Z. , Cheng, T. , Cheng, T. & 3 others Cheng, L., Cheng, L. & Cheng, L. 2015 In : Stem cells translational medicine. 4, 4, p. 320-332 13 p.

Research output: Contribution to journalArticle

Induced Pluripotent Stem Cells
Blood Cells
Paroxysmal Hemoglobinuria
Feeder Cells
Vitronectin

Derivation of megakaryocytes and platelets from human pluripotent stem cells

Li, Y., Wang, Y., Cheng, L. & Wang, Z. Z. Aug 25 2015 Hematopoietic Differentiation of Human Pluripotent Stem Cells. Springer Netherlands, p. 41-50 10 p.

Research output: Chapter in Book/Report/Conference proceedingChapter

Pluripotent Stem Cells
Megakaryocytes
Platelets
Stem cells
Blood Platelets

Early intervention for spinal cord injury with human induced pluripotent stem cells oligodendrocyte progenitors

All, A. H., Gharibani, P., Gupta, S., Bazley, F. A., Pashai, N., Chou, B. K., Shah, S., Resar, L. M., Cheng, L., Gearhart, J. D. & Kerr, C. L. Jan 30 2015 In : PLoS One. 10, 1, e0116933

Research output: Contribution to journalArticle

Induced Pluripotent Stem Cells
Oligodendroglia
Stem cells
Spinal Cord Injuries
spinal cord

Efficient and allele-specific genome editing of disease loci in human iPSCs

Smith, C., Abalde-Atristain, L., He, C., Brodsky, B. R., Braunstein, E. M., Chaudhari, P., Jang, Y. Y., Cheng, L. & Ye, Z. Mar 5 2015 In : Molecular Therapy. 23, 3, p. 570-577 8 p.

Research output: Contribution to journalArticle

Induced Pluripotent Stem Cells
Clustered Regularly Interspaced Short Palindromic Repeats
Alleles
Point Mutation
Guide RNA

Efficient generation of megakaryocytes from human induced pluripotent stem cells using food and drug administration-approved pharmacological reagents

Liu, Y., Wang, Y., Wang, Y., Wang, Y., Gao, Y., Gao, Y., Forbes, J. A., Qayyum, R., Becker, L., Cheng, L., Cheng, L., Wang, Z. Z. & Wang, Z. Z. 2015 In : Stem cells translational medicine. 4, 4, p. 309-319 11 p.

Research output: Contribution to journalArticle

Induced Pluripotent Stem Cells
Megakaryocytes
United States Food and Drug Administration
Pharmacology
oprelvekin

Production of gene-corrected adult beta globin protein in human erythrocytes differentiated from patient ipscs after genome editing of the sickle point mutation

Huang, X., Wang, Y., Yan, W., Smith, C., Ye, Z., Wang, J., Gao, Y., Mendelsohn, L. & Cheng, L. May 1 2015 In : Stem Cells. 33, 5, p. 1470-1479 10 p.

Research output: Contribution to journalArticle

Induced Pluripotent Stem Cells
beta-Globins
Point Mutation
Erythrocytes
Sickle Cell Anemia
2014

A panel of CpG methylation sites distinguishes human embryonic stem cells and induced pluripotent stem cells

Huang, K., Shen, Y., Xue, Z., Bibikova, M., April, C., Liu, Z., Cheng, L., Nagy, A., Pellegrini, M., Fan, J. B. & Fan, G. Jan 14 2014 In : Stem Cell Reports. 2, 1, p. 36-43 8 p.

Research output: Contribution to journalArticle

Induced Pluripotent Stem Cells
Methylation
Stem cells
Gene Silencing
DNA Methylation

Concise review: Stem cell-based approaches to red blood cell production for transfusion

Shah, S., Huang, X. & Cheng, L. 2014 In : Stem cells translational medicine. 3, 3, p. 346-355 10 p.

Research output: Contribution to journalArticle

Erythrocyte Transfusion
Stem Cells
Erythrocytes
Blood Platelets
Blood Component Transfusion

Differential sensitivity to jak inhibitory drugs by isogenic human erythroblasts and hematopoietic progenitors generated from patient-specific induced pluripotent stem cells

Ye, Z., Liu, C. F., Lanikova, L., Dowey, S. N., He, C., Huang, X., Brodsky, R. A., Spivak, J. L., Prchal, J. T. & Cheng, L. Jan 2014 In : Stem Cells. 32, 1, p. 269-278 10 p.

Research output: Contribution to journalArticle

Induced Pluripotent Stem Cells
Erythroblasts
Pharmaceutical Preparations
Polycythemia Vera
Erythropoiesis

Early frameshift mutation in PIGA identified in a large XLID family without neonatal lethality

Belet, S., Fieremans, N., Yuan, X., Van Esch, H., Verbeeck, J., Ye, Z., Cheng, L., Brodsky, B. R., Hu, H., Kalscheuer, V. M., Brodsky, R. A. & Froyen, G. 2014 In : Human Mutation. 35, 3, p. 350-355 6 p.

Research output: Contribution to journalArticle

Glycosylphosphatidylinositols
Frameshift Mutation
Muscle Hypotonia
Seizures
Complementary DNA

Efficient and scalable expansion of human pluripotent stem cells under clinically compliant settings: A view in 2013

Wang, Y., Cheng, L. & Gerecht, S. 2014 In : Annals of Biomedical Engineering. 42, 7, p. 1357-1372 16 p.

Research output: Contribution to journalArticle

Pluripotent Stem Cells
Stem cells
Cell culture
Suspensions
Regenerative Medicine

Extensive ex vivo expansion of functional human erythroid precursors established from umbilical cord blood cells by defined factors

Huang, X., Shah, S., Wang, J., Ye, Z., Dowey, S. N., Tsang, K. M., Mendelsohn, L. G., Kato, G. J., Kickler, T. S. & Cheng, L. Feb 2014 In : Molecular Therapy. 22, 2, p. 451-463 13 p.

Research output: Contribution to journalArticle

Erythroblasts
Fetal Blood
Blood Cells
Hemoglobins
Erythrocytes

Human induced pluripotent stem cell-derived mesenchymal stem cell seeding on calcium phosphate scaffold for bone regeneration

Tang, M., Chen, W., Liu, J., Weir, M. D., Cheng, L. & Xu, H. H. K. Apr 1 2014 In : Tissue Engineering - Part A. 20, 7-8, p. 1295-1305 11 p.

Research output: Contribution to journalArticle

Induced Pluripotent Stem Cells
Bone Regeneration
Calcium phosphate
Scaffolds (biology)
Stem cells

Stimulation of somatic cell reprogramming by ERas-Akt-FoxO1 signaling axis

Yu, Y., Liang, D., Tian, Q., Chen, X., Jiang, B., Chou, B. K., Hu, P., Cheng, L., Gao, P., Li, J. & Wang, G. Feb 2014 In : Stem Cells. 32, 2, p. 349-363 15 p.

Research output: Contribution to journalArticle

Induced Pluripotent Stem Cells
Forkhead Transcription Factors
1-Phosphatidylinositol 4-Kinase
ras Genes
Neoplastic Stem Cells

Synaptic dysregulation in a human iPS cell model of mental disorders

Wen, Z. , Nguyen, H. N. , Guo, Z. , Lalli, M. A. , Wang, X. , Su, Y. , Kim, N. S. , Yoon, K. J. , Shin, J. , Zhang, C. , Makri, G. , Nauen, D. , Yu, H. , Guzman, E. , Chiang, C. H. , Yoritomo, N. , Kaibuchi, K. , Zou, J. , Christian, K. M. , Cheng, L. & 6 others Ross, C. A., Margolis, R. L., Chen, G., Kosik, K. S., Song, H. & Ming, G. L. Nov 20 2014 In : Nature. 515, 7527, p. 414-418 5 p.

Research output: Contribution to journalArticle

Induced Pluripotent Stem Cells
Mental Disorders
Psychiatry
Schizophrenia
Synapses

Targeted correction of RUNX1 mutation in FPD patient-specific induced pluripotent stem cells rescues megakaryopoietic defects

Connelly, J. P., Kwon, E. M., Gao, Y., Trivedi, N. S., Elkahloun, A. G., Horwitz, M. S., Cheng, L. & Liu, P. P. Sep 18 2014 In : Blood. 124, 12, p. 1926-1930 5 p.

Research output: Contribution to journalArticle

Induced Pluripotent Stem Cells
Platelets
Stem cells
Acute Myeloid Leukemia
Blood Platelets

Whole-genome sequencing analysis reveals high specificity of CRISPR/Cas9 and TALEN-based genome editing in human iPSCs

Smith, C., Gore, A., Yan, W., Abalde-Atristain, L., Li, Z., He, C., Wang, Y., Brodsky, R. A., Zhang, K., Cheng, L. & Ye, Z. Jul 3 2014 In : Cell Stem Cell. 15, 1, p. 12-13 2 p.

Research output: Contribution to journalArticle

CRISPR-Cas Systems
Clustered Regularly Interspaced Short Palindromic Repeats
Induced Pluripotent Stem Cells
Genetic Engineering
Endonucleases

Whole-genome sequencing identifies genetic variances in culture-expanded human mesenchymal stem cells

Cai, J., Miao, X., Li, Y., Smith, C., Tsang, K., Cheng, L. & Wang, Q. F. Aug 12 2014 In : Stem Cell Reports. 3, 2, p. 227-233 7 p.

Research output: Contribution to journalArticle

Stem cells
Mesenchymal Stromal Cells
Cell culture
Genes
Genome
2013

And then there were none: No need for pluripotency factors to induce reprogramming

Chou, B. K. & Cheng, L. Sep 5 2013 In : Cell Stem Cell. 13, 3, p. 261-262 2 p.

Research output: Contribution to journalArticle

GATA3 Transcription Factor
Octamer Transcription Factor-3
Cell Dedifferentiation
Small Molecule Libraries
Cell Engineering

Expanded activity of dimer nucleases by combining ZFN and TALEN for genome editing

Yan, W., Smith, C. & Cheng, L. 2013 In : Scientific Reports. 3, 2376

Research output: Contribution to journalArticle

Zinc Fingers
Gene Targeting
Plant Genome
Induced Pluripotent Stem Cells
Double-Stranded DNA Breaks

Generation and homing of iPSC-derived hematopoietic cells in vivo

Chou, B. K., Ye, Z. & Cheng, L. Jul 2013 In : Molecular Therapy. 21, 7, p. 1292-1293 2 p.

Research output: Contribution to journalArticle

Induced Pluripotent Stem Cells
Teratoma
Hematopoietic Stem Cells
Induced Pluripotent Stem Cells
Glycosylphosphatidylinositols
Blood Cells
Proteins
Bone Morphogenetic Protein 4

Response: the role of RUNX1 isoforms in hematopoietic commitment of human pluripotent stem cells.

Ran, D., Lam, K., Shia, W. J., Lo, M. C., Fan, J. B., Knorr, D. A., Ferrell, P. I., Ye, Z., Yan, M., Cheng, L., Kaufman, D. S. & Zhang, D. E. Jun 27 2013 In : Blood. 121, 26, p. 5252-5253 2 p.

Research output: Contribution to journalArticle

RUNX1a enhances hematopoietic lineage commitment from human embryonic stem cells and inducible pluripotent stem cells

Ran, D., Shia, W. J., Lo, M. C., Fan, J. B., Knorr, D. A., Ferrell, P. I., Ye, Z., Yan, M., Cheng, L., Kaufman, D. S. & Zhang, D. E. Apr 11 2013 In : Blood. 121, 15, p. 2882-2890 9 p.

Research output: Contribution to journalArticle

Pluripotent Stem Cells
Hematopoietic Stem Cells
Stem cells
Embryoid Bodies
Hematopoiesis

Scalable expansion of human induced pluripotent stem cells in the defined xeno-free E8 medium under adherent and suspension culture conditions

Wang, Y., Chou, B. K., Dowey, S., He, C., Gerecht, S. & Cheng, L. Nov 2013 In : Stem Cell Research. 11, 3, p. 1103-1116 14 p.

Research output: Contribution to journalArticle

Induced Pluripotent Stem Cells
Suspensions
Cryopreservation
Karyotype
Economics

Self-organized vascular networks from human pluripotent stem cells in a synthetic matrix

Kusuma, S., Shen, Y. I., Hanjaya-Putra, D., Mali, P., Cheng, L. & Gerecht, S. Jul 30 2013 In : Proceedings of the National Academy of Sciences of the United States of America. 110, 31, p. 12601-12606 6 p.

Research output: Contribution to journalArticle

Pluripotent Stem Cells
Blood Vessels
Pericytes
Microvessels
Endothelial Cells
2012

Concise review: Human cell engineering: Cellular reprogramming and genome editing

Mali, P. & Cheng, L. Jan 2012 In : Stem Cells. 30, 1, p. 75-81 7 p.

Research output: Contribution to journalArticle

Cell Engineering
Aptitude
Human Engineering
Induced Pluripotent Stem Cells
Regenerative Medicine

Efficient derivation and genetic modifications of human pluripotent stem cells on engineered human feeder cell lines

Zou, C., Chou, B. K., Dowey, S. N., Tsang, K., Huang, X., Liu, C. F., Smith, C., Yen, J., Mali, P., Zhang, Y. A., Cheng, L. & Ye, Z. Aug 10 2012 In : Stem Cells and Development. 21, 12, p. 2298-2311 14 p.

Research output: Contribution to journalArticle

Feeder Cells
Pluripotent Stem Cells
Medical Genetics
Cell Line
Fibroblasts

Generation of integration-free human induced pluripotent stem cells from postnatal blood mononuclear cells by plasmid vector expression

Dowey, S. N., Huang, X., Chou, B. K., Ye, Z. & Cheng, L. Nov 2012 In : Nature Protocols. 7, 11, p. 2013-2021 9 p.

Research output: Contribution to journalArticle

Induced Pluripotent Stem Cells
Stem cells
Blood Cells
Plasmids
Blood

Low incidence of DNA sequence variation in human induced pluripotent stem cells generated by nonintegrating plasmid expression

Cheng, L., Hansen, N. F., Zhao, L., Du, Y., Zou, C., Donovan, F. X., Chou, B. K., Zhou, G., Li, S., Dowey, S. N., Ye, Z., Chandrasekharappa, S. C., Yang, H., Mullikin, J. C. & Liu, P. P. Mar 2 2012 In : Cell Stem Cell. 10, 3, p. 337-344 8 p.

Research output: Contribution to journalArticle

Induced Pluripotent Stem Cells
Plasmids
Incidence
Cell Line
Genome

Promise and challenges of human iPSC-based hematologic disease modeling and treatment

Ye, Z., Chou, B. K. & Cheng, L. Jun 2012 In : International Journal of Hematology. 95, 6, p. 601-609 9 p.

Research output: Contribution to journalArticle

Hematologic Diseases
Hematopoietic Stem Cells
Bone Marrow
Pluripotent Stem Cells
Hematopoietic Stem Cell Transplantation

The differential formation of the LINC-mediated perinuclear actin cap in pluripotent and somatic cells

Khatau, S. B., Kusuma, S., Hanjaya-Putra, D., Mali, P., Cheng, L., Lee, J. S. H., Gerecht, S. & Wirtz, D. May 4 2012 In : PLoS One. 7, 5, e36689

Research output: Contribution to journalArticle

cytoskeleton
Cytoskeleton
somatic cells
actin
Actins

The phenotype of a germline mutation in PIGA: The gene somatically mutated in paroxysmal nocturnal hemoglobinuria

Johnston, J. J., Gropman, A. L., Sapp, J. C., Teer, J. K., Martin, J. M., Liu, C. F., Yuan, X., Ye, Z., Cheng, L., Brodsky, R. A. & Biesecker, L. G. Feb 10 2012 In : American Journal of Human Genetics. 90, 2, p. 295-300 6 p.

Research output: Contribution to journalArticle

Paroxysmal Hemoglobinuria
Glycosylphosphatidylinositols
Germ-Line Mutation
Phenotype
Genes
2011

Efficient human iPS cell derivation by a non-integrating plasmid from blood cells with unique epigenetic and gene expression signatures

Chou, B. K., Mali, P., Huang, X., Ye, Z., Dowey, S. N., Resar, L. M. S., Zou, C., Zhang, Y. A., Tong, J. & Cheng, L. Mar 2011 In : Cell Research. 21, 3, p. 518-529 12 p.

Research output: Contribution to journalArticle

Induced Pluripotent Stem Cells
Transcriptome
Epigenomics
Blood Cells
Plasmids

Hematopoietic stem/progenitor cells, generation of induced pluripotent stem cells, and isolation of endothelial progenitors from 21- to 23.5-year cryopreserved cord blood

Broxmeyer, H. E., Lee, M. R., Hangoc, G., Cooper, S., Prasain, N., Kim, Y. J., Mallett, C., Ye, Z., Witting, S., Cornetta, K., Cheng, L. & Yoder, M. C. May 5 2011 In : Blood. 117, 18, p. 4773-4777 5 p.

Research output: Contribution to journalArticle

Induced Pluripotent Stem Cells
Cell Separation
Hematopoietic Stem Cells
Stem cells
Fetal Blood