Gyanu Lamichhane

Associate Professor

20032019
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Research Output 2003 2019

2019

Identification of potent L,D-transpeptidase 5 inhibitors for Mycobacterium tuberculosis as potential anti-TB leads: virtual screening and molecular dynamics simulations

Sabe, V. T., Tolufashe, G. F., Ibeji, C. U., Maseko, S. B., Govender, T., Maguire, G. E. M., Lamichhane, G., Honarparvar, B. & Kruger, H. G., Nov 1 2019, In : Journal of Molecular Modeling. 25, 11, 328.

Research output: Contribution to journalArticle

Peptidyl Transferases
tuberculosis
inhibitors
Molecular dynamics
Screening

Inhibition mechanism of L,D-transpeptidase 5 in presence of the β-lactams using ONIOM method

Tolufashe, G. F., Sabe, V. T., Ibeji, C. U., Lawal, M. M., Govender, T., Maguire, G. E. M., Lamichhane, G., Kruger, H. G. & Honarparvar, B., Mar 1 2019, In : Journal of Molecular Graphics and Modelling. 87, p. 204-210 7 p.

Research output: Contribution to journalArticle

Peptidyl Transferases
acylation
Lactams
Acylation
tuberculosis
Lactams
Mycobacterium
meropenem
Cefuroxime
Carbapenems

N-Trifluoromethylthiolated Sulfonimidamides and Sulfoximines: Anti-microbial, Anti-mycobacterial, and Cytotoxic Activity

Thota, N., Makam, P., Rajbongshi, K. K., Nagiah, S., Abdul, N. S., Chuturgoon, A. A., Kaushik, A., Lamichhane, G., Somboro, A. M., Kruger, H. G., Govender, T., Naicker, T. & Arvidsson, P. I., Oct 10 2019, In : ACS Medicinal Chemistry Letters. 10, 10, p. 1457-1461 5 p.

Research output: Contribution to journalArticle

Ethambutol
Bacilli
Escherichia coli
Inhibitory Concentration 50
Toxicity

Select -Lactam Combinations Exhibit Synergy against Mycobacterium abscessus In Vitro

Story-Roller, E., Maggioncalda, E. C. & Lamichhane, G., Apr 1 2019, In : Antimicrobial agents and chemotherapy. e02613-18.

Research output: Contribution to journalArticle

Lactams
Mycobacterium
Anti-Bacterial Agents
Peptidyl Transferases
Nontuberculous Mycobacteria

Synergistic Efficacy of β-Lactam Combinations against Mycobacterium abscessus Pulmonary Infection in Mice

Story-Roller, E., Maggioncalda, E. C. & Lamichhane, G., Aug 1 2019, In : Antimicrobial agents and chemotherapy. 63, 8

Research output: Contribution to journalArticle

Lactams
Mycobacterium
Lung
Lung Diseases
Infection

The Driving Force for the Acylation of β-Lactam Antibiotics by L,D-Transpeptidase 2: Quantum Mechanics/Molecular Mechanics (QM/MM) Study

Ibeji, C. U., Lawal, M. M., Tolufashe, G. F., Govender, T., Naicker, T., Maguire, G. E. M., Lamichhane, G., Kruger, H. G. & Honarparvar, B., Jan 1 2019, In : ChemPhysChem.

Research output: Contribution to journalArticle

Peptidyl Transferases
acylation
Lactams
Acylation
Molecular mechanics
2018

Activities of dual combinations of antibiotics against multidrug-resistant nontuberculous mycobacteria recovered from patients with cystic fibrosis

Schwartz, M., Fisher, S., Story-Roller, E., Lamichhane, G. & Parrish, N. M., Oct 1 2018, In : Microbial Drug Resistance. 24, 8, p. 1191-1197 7 p.

Research output: Contribution to journalArticle

Nontuberculous Mycobacteria
Cystic Fibrosis
Anti-Bacterial Agents
Microbial Sensitivity Tests
Mycobacterium chelonae

Have we realized the full potential of β-lactams for treating drug-resistant TB?

Story-Roller, E. & Lamichhane, G., Sep 1 2018, In : IUBMB Life. 70, 9, p. 881-888 8 p.

Research output: Contribution to journalReview article

Lactams
Pharmaceutical Preparations
Anti-Bacterial Agents
Bacteria

Inhibition of Mycobacterium tuberculosis L,D-Transpeptidase 5 by Carbapenems: MD and QM/MM Mechanistic Studies

Tolufashe, G. F., Halder, A. K., Ibeji, C. U., Lawal, M. M., Ntombela, T., Govender, T., Maguire, G. E. M., Lamichhane, G., Kruger, H. G. & Honarparvar, B., Dec 28 2018, In : ChemistrySelect. 3, 48, p. 13603-13612 10 p.

Research output: Contribution to journalArticle

Peptidyl Transferases
Carbapenems
meropenem
Molecular dynamics
Imipenem

Molecular insight on the non-covalent interactions between carbapenems and l,d-transpeptidase 2 from Mycobacterium tuberculosis: ONIOM study

Ntombela, T., Fakhar, Z., Ibeji, C. U., Govender, T., Maguire, G. E. M., Lamichhane, G., Kruger, H. G. & Honarparvar, B., May 29 2018, (Accepted/In press) In : Journal of Computer-Aided Molecular Design. p. 1-15 15 p.

Research output: Contribution to journalArticle

Peptidyl Transferases
tuberculosis
Carbapenems
Mycobacterium tuberculosis
Hydrogen bonds

Mycobacterium abscessus and β-lactams: Emerging insights and potential opportunities

Story-Roller, E., Maggioncalda, E. C., Cohen, K. & Lamichhane, G., Sep 25 2018, In : Frontiers in Microbiology. 9, SEP, 2273.

Research output: Contribution to journalReview article

Lactams
Mycobacterium
Anti-Bacterial Agents
Nontuberculous Mycobacteria
Mycobacterium Infections

The catalytic role of water in the binding site of L,D-transpeptidase 2 within acylation mechanism: A QM/MM (ONIOM) modelling

Ibeji, C. U., Tolufashe, G. F., Ntombela, T., Govender, T., Maguire, G. E. M., Lamichhane, G., Kruger, H. G. & Honarparvar, B., Dec 1 2018, In : Tuberculosis. 113, p. 222-230 9 p.

Research output: Contribution to journalArticle

Peptidyl Transferases
Acylation
Lactams
Peptidoglycan
Binding Sites
2017

Combinations of avibactam and carbapenems exhibit enhanced potencies against drug-resistant Mycobacterium abscessus

Kaushik, A., Gupta, C., Fisher, S., Story-Roller, E., Galanis, C., Parrish, N. M. & Lamichhane, G., May 1 2017, In : Future Microbiology. 12, 6, p. 473-480 8 p.

Research output: Contribution to journalArticle

Carbapenems
Mycobacterium
Pharmaceutical Preparations
Lactams
Infection

Computational model for the acylation step of the β-lactam ring: Potential application for L,D-transpeptidase 2 in mycobacterium tuberculosis

Fakhar, Z., Govender, T., Lamichhane, G., Maguire, G. E. M., Kruger, H. G. & Honarparvar, B., Jan 15 2017, In : Journal of Molecular Structure. 1128, p. 94-102 9 p.

Research output: Contribution to journalArticle

Peptidyl Transferases
Lactams
Acylation
Carbapenems
Molecular orbitals

Differential flap dynamics in l,d-transpeptidase2 from mycobacterium tuberculosis revealed by molecular dynamics

Fakhar, Z., Govender, T., Maguire, G. E. M., Lamichhane, G., Walker, R. C., Kruger, H. G. & Honarparvar, B., 2017, In : Molecular BioSystems. 13, 6, p. 1223-1234 12 p.

Research output: Contribution to journalArticle

Carbapenems
meropenem
Molecular Dynamics Simulation
Mycobacterium tuberculosis
Ligands

Drug resistance mechanisms and novel drug targets for tuberculosis therapy

Islam, M. M., Hameed, H. M. A., Mugweru, J., Chhotaray, C., Wang, C., Tan, Y., Liu, J., Li, X., Tan, S., Ojima, I., Yew, W. W., Nuermberger, E., Lamichhane, G. & Zhang, T., Jan 20 2017, In : Journal of Genetics and Genomics. 44, 1, p. 21-37 17 p.

Research output: Contribution to journalReview article

Drug Resistance
Tuberculosis
Pharmaceutical Preparations
Multidrug-Resistant Tuberculosis
Therapeutics

Emerging therapies

Lamichhane, G. & Grosset, J. H., Mar 30 2017, Handbook of Tuberculosis. Springer International Publishing, p. 191-218 28 p.

Research output: Chapter in Book/Report/Conference proceedingChapter

Therapy

Inhibition of innate immune cytosolic surveillance by an M.Tuberculosis phosphodiesterase

Dey, R. J., Dey, B., Zheng, Y., Cheung, L. S., Zhou, J., Sayre, D., Kumar, P., Guo, H., Lamichhane, G., Sintim, H. O. & Bishai, W. R., Feb 1 2017, In : Nature Chemical Biology. 13, 2, p. 210-217 8 p.

Research output: Contribution to journalArticle

Phosphoric Diester Hydrolases
Interferon Type I
Tuberculosis
Mycobacterium Infections
Phosphodiesterase Inhibitors

In vitro and in vivo activity of biapenem against drug-susceptible and rifampicin-resistant Mycobacterium tuberculosis

Kaushik, A., Ammerman, N., Tasneen, R., Story-Roller, E., Dooley, K. E., Dorman, S. E., Nuermberger, E. & Lamichhane, G., Aug 1 2017, In : Journal of Antimicrobial Chemotherapy. 72, 8, p. 2320-2325 6 p., dkx152.

Research output: Contribution to journalArticle

biapenem
Rifampin
Mycobacterium tuberculosis
Pharmaceutical Preparations
In Vitro Techniques

LdtMav2, a nonclassical transpeptidase and susceptibility of Mycobacterium avium to carbapenems

Mattoo, R., Lloyd, E. P., Kaushik, A., Kumar, P., Brunelle, J. L., Townsend, C. A. & Lamichhane, G., Jun 1 2017, In : Future Microbiology. 12, 7, p. 595-607 13 p.

Research output: Contribution to journalArticle

Peptidyl Transferases
Mycobacterium avium
Carbapenems
Lactams
Mycobacterium Infections

Mutation in an unannotated protein confers carbapenem resistance in Mycobacterium tuberculosis

Kumar, P., Kaushik, A., Bell, D. T., Chauhan, V., Xia, F., Stevens, R. L. & Lamichhane, G., Mar 1 2017, In : Antimicrobial Agents and Chemotherapy. 61, 3, e02234.

Research output: Contribution to journalArticle

Lactams
Carbapenems
Mycobacterium tuberculosis
Mutation
meropenem

Mycobacterium abscessus L,D-transpeptidases are susceptible to inactivation by carbapenems and cephalosporins but not penicillins

Kumar, P., Chauhan, V., Silva, J. R. A., Lameira, J., D’Andrea, F. B., Li, S. G., Ginell, S. L., Freundlich, J. S., Alves, C. N., Bailey, S., Cohen, K. & Lamichhane, G., Oct 1 2017, In : Antimicrobial Agents and Chemotherapy. 61, 10, e00866.

Research output: Contribution to journalArticle

Peptidyl Transferases
Lactams
Carbapenems
Cephalosporins
Mycobacterium

Pyrazinoic acid inhibits a bifunctional enzyme in Mycobacterium tuberculosis

Njire, M., Wang, N., Wang, B., Tan, Y., Cai, X., Liu, Y., Mugweru, J., Guo, J., Hameed, H. M. A., Tan, S., Liu, J., Yew, W. W., Nuermberger, E., Lamichhane, G., Liu, J. & Zhang, T., Jul 1 2017, In : Antimicrobial Agents and Chemotherapy. 61, 7, e00070-17.

Research output: Contribution to journalArticle

Pyrazinamide
Mycobacterium tuberculosis
Enzymes
Alleles
Pharmaceutical Preparations

Structural insight into the inactivation of Mycobacterium tuberculosis non-classical transpeptidase LdtMt2 by biapenem and tebipenem

Bianchet, M. A., Pan, Y. H., Basta, L. A. B., Saavedra, H., Lloyd, E. P., Kumar, P., Mattoo, R., Townsend, C. A. & Lamichhane, G., May 25 2017, In : BMC Biochemistry. 18, 1, 8.

Research output: Contribution to journalArticle

biapenem
Peptidyl Transferases
Carbapenems
Mycobacterium tuberculosis
Lactams

Substituted N-Phenyl-5-(2-(phenylamino)thiazol-4-yl)isoxazole-3-carboxamides Are Valuable Antitubercular Candidates that Evade Innate Efflux Machinery

Azzali, E., Machado, D., Kaushik, A., Vacondio, F., Flisi, S., Cabassi, C. S., Lamichhane, G., Viveiros, M., Costantino, G. & Pieroni, M., Aug 24 2017, In : Journal of Medicinal Chemistry. 60, 16, p. 7108-7122 15 p.

Research output: Contribution to journalArticle

Isoxazoles
Mycobacterium tuberculosis
Antitubercular Agents
Eukaryotic Cells
Structure-Activity Relationship
2016

A comparative modeling and molecular docking study on Mycobacterium tuberculosis targets involved in peptidoglycan biosynthesis

Fakhar, Z., Naiker, S., Alves, C. N., Govender, T., Maguire, G. E. M., Lameira, J., Lamichhane, G., Kruger, H. G. & Honarparvar, B., Apr 3 2016, (Accepted/In press) In : Journal of Biomolecular Structure and Dynamics. p. 1-19 19 p.

Research output: Contribution to journalArticle

Peptidoglycan
Mycobacterium tuberculosis
Multidrug-Resistant Tuberculosis
Protein Stability
Enzymes

An evolved oxazolidinone with selective potency against Mycobacterium tuberculosis and gram positive bacteria

Kaushik, A., Heuer, A. M., Bell, D. T., Culhane, J. C., Ebner, D. C., Parrish, N. M., Ippoliti, J. T. & Lamichhane, G., Apr 19 2016, (Accepted/In press) In : Bioorganic and Medicinal Chemistry Letters.

Research output: Contribution to journalArticle

Oxazolidinones
Gram-Positive Bacteria
Mycobacterium tuberculosis
Bacteria
Enterococcus faecalis

Carbapenems against Mycobacterium tuberculosis: A review of the evidence

Jaganath, D., Lamichhane, G. & Shah, M., Nov 1 2016, In : International Journal of Tuberculosis and Lung Disease. 20, 11, p. 1436-1447 12 p.

Research output: Contribution to journalReview article

Carbapenems
Mycobacterium tuberculosis
Tuberculosis
Extensively Drug-Resistant Tuberculosis
Lactams

Non-classical transpeptidases yield insight into new antibacterials

Kumar, P., Kaushik, A., Lloyd, E. P., Li, S. G., Mattoo, R., Ammerman, N., Bell, D. T., Perryman, A. L., Zandi, T. A., Ekins, S., Ginell, S. L., Townsend, C. A., Freundlich, J. S. & Lamichhane, G., Nov 7 2016, (Accepted/In press) In : Nature Chemical Biology.

Research output: Contribution to journalArticle

Peptidyl Transferases
Peptidoglycan
Lactams
Carbapenems
4 alpha-glucanotransferase

REMap: Operon map of M. tuberculosis based on RNA sequence data

Pelly, S., Winglee, K., Xia, F., Stevens, R. L., Bishai, W. R. & Lamichhane, G., Jul 1 2016, In : Tuberculosis. 99, p. 70-80 11 p.

Research output: Contribution to journalArticle

Operon
Tuberculosis
RNA
RNA Sequence Analysis
Intergenic DNA

Role of the Cys154Arg substitution in ribosomal protein L3 in oxazolidinone resistance in Mycobacterium tuberculosis

Makafe, G. G., Cao, Y., Tan, Y., Julius, M., Liu, Z., Wang, C., Njire, M. M., Cai, X., Liu, T., Wang, B., Pang, W., Tan, S., Zhang, B., Yew, W. W., Lamichhane, G., Guo, J. & Zhang, T., May 1 2016, In : Antimicrobial Agents and Chemotherapy. 60, 5, p. 3202-3206 5 p.

Research output: Contribution to journalArticle

Linezolid
Oxazolidinones
Mycobacterium tuberculosis
Mycobacterium
Genes

Targeting the cell wall of Mycobacterium tuberculosis: A molecular modeling investigation of the interaction of imipenem and meropenem with L,D-transpeptidase 2

Silva, J. R. A., Bishai, W. R., Govender, T., Lamichhane, G., Maguire, G. E. M., Kruger, H. G., Lameira, J. & Alves, C. N., 2016, In : Journal of Biomolecular Structure and Dynamics. 34, 2, p. 304-317 14 p.

Research output: Contribution to journalArticle

meropenem
Peptidyl Transferases
Imipenem
Mycobacterium tuberculosis
Cell Wall
2015

Carbapenems and Rifampin exhibit synergy against Mycobacterium tuberculosis and Mycobacterium abscessus

Kaushik, A., Makkar, N., Pandey, P., Parrish, N. M., Singh, U. & Lamichhane, G., Oct 1 2015, In : Antimicrobial Agents and Chemotherapy. 59, 10, p. 6561-6567 7 p.

Research output: Contribution to journalArticle

Carbapenems
Rifampin
Mycobacterium
Mycobacterium tuberculosis
meropenem

Immunogenicity without efficacy of an adenoviral tuberculosis vaccine in a stringent mouse model for immunotherapy during treatment

Alyahya, S. A., Nolan, S. T., Smith, C. M. R., Bishai, W. R., Sadoff, J. & Lamichhane, G., May 21 2015, In : PLoS One. 10, 5, e0127907.

Research output: Contribution to journalArticle

Tuberculosis Vaccines
immunotherapy
tuberculosis
Immunotherapy
Immunity

Loss of a functionally and structurally distinct LD-transpeptidase, LdtMt5, compromises cell wall integrity in mycobacterium tuberculosis

Basta, L. A. B., Ghosh, A., Pan, Y., Jakoncic, J., Lloyd, E. P., Townsend, C. A., Lamichhane, G. & Bianchet, M. A., Oct 16 2015, In : Journal of Biological Chemistry. 290, 42, p. 25670-25685 16 p.

Research output: Contribution to journalArticle

Peptidyl Transferases
Peptidoglycan
Mycobacterium tuberculosis
Cell Wall
meropenem

Structural and functional features of enzymes of Mycobacterium tuberculosis peptidoglycan biosynthesis as targets for drug development

Moraes, G. L., Gomes, G. C., Monteiro De Sousa, P. R., Alves, C. N., Govender, T., Kruger, H. G., Maguire, G. E. M., Lamichhane, G. & Lameira, J., Mar 1 2015, In : Tuberculosis. 95, 2, p. 95-111 17 p.

Research output: Contribution to journalArticle

Peptidoglycan
Mycobacterium tuberculosis
Tuberculosis
Enzymes
Pharmaceutical Preparations
2014

Systems biology-based identification of Mycobacterium tuberculosis persistence genes in mouse lungs

Dutta, N., Bandyopadhyay, N., Veeramani, B., Lamichhane, G., Karakousis, P. & Bader, J. S., Feb 18 2014, In : mBio. 5, 1, e01066-13.

Research output: Contribution to journalArticle

Systems Biology
Mycobacterium tuberculosis
Lung
Genes
Tuberculosis
2013

Definition and annotation of (myco)bacterial non-coding RNA

Lamichhane, G., Arnvig, K. B. & McDonough, K. A., Jan 2013, In : Tuberculosis. 93, 1, p. 26-29 4 p.

Research output: Contribution to journalArticle

Bacterial RNA
Untranslated RNA
RNA
Bacteria
Mycobacterium
2012

A screen for non-coding RNA in Mycobacterium tuberculosis reveals a cAMP-responsive RNA that is expressed during infection

Pelly, S., Bishai, W. R. & Lamichhane, G., May 25 2012, In : Gene. 500, 1, p. 85-92 8 p.

Research output: Contribution to journalArticle

Small Untranslated RNA
Untranslated RNA
Mycobacterium tuberculosis
RNA
Infection

Combining cheminformatics methods and pathway analysis to identify molecules with whole-cell activity against mycobacterium tuberculosis

Sarker, M., Talcott, C., Madrid, P., Chopra, S., Bunin, B. A., Lamichhane, G., Freundlich, J. S. & Ekins, S., Aug 2012, In : Pharmaceutical Research. 29, 8, p. 2115-2127 13 p.

Research output: Contribution to journalArticle

Mycobacterium tuberculosis
Tuberculosis
Thiourea
Molecules
Pharmaceutical Databases

Targeting the cell wall of mycobacterium tuberculosis: Structure and mechanism of L,D-transpeptidase 2

Erdemli, S. B., Gupta, R., Bishai, W. R., Lamichhane, G., Amzel, M. L. & Bianchet, M. A., Dec 5 2012, In : Structure. 20, 12, p. 2103-2115 13 p.

Research output: Contribution to journalArticle

Peptidyl Transferases
Mycobacterium tuberculosis
Cell Wall
Peptidoglycan
Mycobacterium
2011

Copper resistance is essential for virulence of Mycobacterium tuberculosis

Wolschendorf, F., Ackart, D., Shrestha, T. B., Hascall-Dove, L., Nolan, S., Lamichhane, G., Wang, Y., Bossmann, S. H., Basaraba, R. J. & Niederweis, M., Jan 25 2011, In : Proceedings of the National Academy of Sciences of the United States of America. 108, 4, p. 1621-1626 6 p.

Research output: Contribution to journalArticle

Mycobacterium tuberculosis
Virulence
Copper
Carrier Proteins
Guinea Pigs

Essential metabolites of mycobacterium tuberculosis and their mimics

Lamichhane, G., Freundlich, J. S., Ekins, S., Wickramaratne, N., Nolan, S. T. & Bishai, W. R., Jan 2011, In : mBio. 2, 1

Research output: Contribution to journalArticle

Mycobacterium tuberculosis
Enzymes
Methionine Sulfoximine
Essential Genes
Growth
Reactive Nitrogen Species
Mycobacterium tuberculosis
Reactive Oxygen Species

Novel targets in M. tuberculosis: Search for new drugs

Lamichhane, G., Jan 2011, In : Trends in Molecular Medicine. 17, 1, p. 25-33 9 p.

Research output: Contribution to journalArticle

Mycobacterium tuberculosis
Tuberculosis
Pharmaceutical Preparations
Molecular Biology
Genome
2010

Genetic requirements for the survival of tubercle bacilli in primates

Dutta, N., Mehra, S., Didier, P. J., Roy, C. J., Doyle, L. A., Alvarez, X., Ratterree, M., Be, N. A., Lamichhane, G., Jain, S., Lacey, M. R., Lackner, A. A. & Kaushal, D., Jun 1 2010, In : Journal of Infectious Diseases. 201, 11, p. 1743-1752 10 p.

Research output: Contribution to journalArticle

Mycobacterium tuberculosis
Bacillus
Primates
Survival
Tuberculosis

Methionine Aminopeptidases from Mycobacterium tuberculosis as Novel Antimycobacterial Targets

Olaleye, O., Raghunand, T. R., Bhat, S., He, J., Tyagi, S., Lamichhane, G., Gu, P., Zhou, J., Zhang, Y., Grosset, J., Bishai, W. R. & Liu, J., Jan 29 2010, In : Chemistry and Biology. 17, 1, p. 86-97 12 p.

Research output: Contribution to journalArticle

Aminopeptidases
Mycobacterium tuberculosis
Methionine
Metalloproteases
Enzymes

Protective efficacy of BCG overexpressing an L,D-transpeptidase against M. tuberculosisinfection

Nolan, S. T. & Lamichhane, G., 2010, In : PLoS One. 5, 10, e13773.

Research output: Contribution to journalArticle

Peptidyl Transferases
tuberculosis
Tuberculosis Vaccines
Nutrients
Tuberculosis

The Impact of mouse passaging of Mycobacterium tuberculosis strains prior to virulence testing in the mouse and guinea pig aerosol models

Converse, P. J., Eisenach, K. D., Theus, S. A., Nuermberger, E., Tyagi, S., Ly, L. H., Geiman, D. E., Guo, H., Nolan, S. T., Akar, N. C., Klinkenberg, L. G., Gupta, R., Lun, S., Karakousis, P., Lamichhane, G., McMurray, D. N., Grosset, J. H. & Bishai, W. R., 2010, In : PLoS One. 5, 4, e10292.

Research output: Contribution to journalArticle

Mycobacterium tuberculosis
aerosols
Aerosols
guinea pigs
Virulence